Developing tools to study relationship between oxidative stress in T cell dysfunction

摘要 在慢性病毒感染或肿瘤微环境中，抗原(Ag)特异性CD8 T细胞 经历另一种激活过程，称为耗竭。疲惫的CD8 T细胞失去增殖能力， 效应器基因表达，从而允许病毒感染或肿瘤生长持续存在，尽管最初 抗原特异性克隆。尽管驱动T细胞耗尽的确切机制尚不清楚，但文献 提示T细胞暴露在过度的氧化应激下有助于建立疲惫。 在这一小笔赠款中，我们建议培育出转基因小鼠，以便于将 暴露在氧化应激下，并将这些工具用于未来T细胞耗竭的研究。

Abstract In response to chronic viral infection or in tumor microenvironment, antigen(Ag)-specific CD8 T cells undergo alternative activation processes, called exhaustion. Exhausted CD8 T cells lose their proliferation and effector gene expression, thus allowing viral infection or tumor growth to persist despite the initial expansion of antigen-specific clones. Although the precise mechanism driving T cell exhaustion is unknown, literature suggests that exposure of T cells to excessive oxidative stress contribution to the establishment of exhaustion. In this small grant, we propose to generate genetically modified mice that facilitate visualization of cells that are exposed to oxidative stress and use the tools for future studies for T cell exhaustion.