Developing tools to study relationship between oxidative stress in T cell dysfunction

摘要 在慢性病毒感染或肿瘤微环境中，抗原（Ag）特异性CD8 T细胞 经历交替的激活过程，称为耗尽。耗尽的CD8 T细胞失去其增殖， 效应基因表达，从而允许病毒感染或肿瘤生长持续，尽管最初的扩张， 抗原特异性克隆。虽然驱动T细胞耗竭的确切机制是未知的，但文献 表明T细胞暴露于过度的氧化应激有助于疲劳的建立。 在这一小笔赠款中，我们建议生产转基因小鼠，以促进细胞的可视化， 暴露于氧化应激，并使用工具进行T细胞耗竭的未来研究。

Abstract In response to chronic viral infection or in tumor microenvironment, antigen(Ag)-specific CD8 T cells undergo alternative activation processes, called exhaustion. Exhausted CD8 T cells lose their proliferation and effector gene expression, thus allowing viral infection or tumor growth to persist despite the initial expansion of antigen-specific clones. Although the precise mechanism driving T cell exhaustion is unknown, literature suggests that exposure of T cells to excessive oxidative stress contribution to the establishment of exhaustion. In this small grant, we propose to generate genetically modified mice that facilitate visualization of cells that are exposed to oxidative stress and use the tools for future studies for T cell exhaustion.