Developing efficient and non-transgenic transformation methods for sterile and/or recalcitrant crops

开发针对不育和/或顽固作物的高效非转基因转化方法

• 批准号: 10107465
• 金额: $ 12.69万
• 依托单位: TROPIC BIOSCIENCES UK LIMITED
• 依托单位国家: 英国
• 项目类别: Launchpad
• 财政年份: 2024
• 资助国家: 英国
• 起止时间: 2024 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=10107465
• 关键词: Developing; efficient; non; transgenic; transformation

Facing the triple challenge of feeding a growing population, dealing with the impacts of climate change, and preventing major losses to emerging pathogens, gene-editing technologies are increasingly seen as playing a key role in achieving future global food security. Unlike genetically modified organisms (GMO), which contain genetic changes that could not have occurred through traditional breeding or arisen naturally, regional regulations are increasingly permissive for gene editing, which focuses on genetic changes that could have arisen through traditional breeding methods or occurred naturally.The UK is a global leader in genetics and genomics research and this position has recently been underpinned by the entry into force of world-leading gene-editing regulations through the Genetic Technology (Precision Breeding) Act 2023, which permits the development and marketing of gene-edited crops and livestock in England.Founded in 2016, Tropic is a pioneering agricultural-biotechnology company developing advanced plant breeding and gene editing technologies, employing over 140 industry-leading professionals at our headquarters in the Norwich Research Park Innovation Centre. We are developing high-performing commercial varieties of tropical crops in-house (focusing initially on banana, rice, and coffee) and, outside tropical crops, making our proprietary and patented gene-editing technology platform available to select partners including leading global agribusinesses BASF, Corteva Agriscience, and Genus.Banana is the world's fourth largest crop by production volume, playing a vital role in global food security and ensuring the livelihoods of millions of small-scale farmers. We are developing gene-edited bananas with valuable traits including extended shelf-life and non-browning, as well as resistance to tropical race 4 (TR4) and black-sigatoka disease (BSD). Banana browning is responsible for the loss of over 60% of exported bananas in the supply chain (i.e. before reaching the consumer). TR4 and BSD are rapidly spreading fungal pathogens that threaten global banana production, with BSD already causing global annual banana production losses of £1.1 billion and BSD management accounting for 27% of banana production costs.Since cultivated bananas are triploid and sterile, new varieties cannot be produced through conventional breeding. While gene editing offers significant promise for valuable trait development, gene editing of banana faces significant implementation challenges including low transformation efficiencies and the requirement to develop non-transgenic transformation methods (since transgenes cannot be bred out).With Innovate UK funding, we will develop efficient and non-transgenic transformation methods for sterile and/or recalcitrant crops, focusing initially on banana as a model crop system.

面对养活人口增长，应对气候变化的影响的三重挑战，并防止对新兴病原体的重大损失，基因编辑技术越来越被视为在实现未来的全球粮食安全方面发挥了关键作用。与遗传变化不同的遗传变化与传统育种或自然产生的遗传变化不同，区域法规对基因编辑的越来越允许，该法规越来越允许，这些遗传变化的重点是通过传统繁殖方法或这种遗传学的遗传变化。通过《遗传技术（Precision Breeping）法》 2023年的基因编辑法规，该法可以开发和营销基因编辑的农作物和牲畜的开发和营销。2016年，热带地区是一家开创性的农业生物技术公司，开发了高级工厂育种技术和基因育种技术，在140个行业中雇用了140个行业的工具，并在我们的140个行业中，我们在我们的活动中均在我们的活动中进行了专业的专业。 We are developing high-performing commercial variations of tropical crops in-house (focusing initially on banana, rice, and coffee) and, outside tropical crops, making our proprietary and patented gene-editing technology platform available to select partners including leading global agribusinesses BASF, Corteva Agriscience, and Genus.Banana is the world's fourth largest crop by production volume, playing a vital role in global food security and ensuring数百万小型农民的生计。我们正在开发具有有价值的特征的基因编辑的香蕉，包括延长的保质期和非褐变，以及对热带种族4（TR4）和黑色sigatoka病（BSD）的抵抗。香蕉勃朗宁（Banana Browning）负责供应链中60％以上的出口香蕉（即在到达消费者之前）。 TR4和BSD正在迅速传播威胁全球香蕉生产的真菌病原体，BSD已经导致全球每年的香蕉生产损失为11亿英镑，而BSD管理占香蕉生产成本的27％。培养的香蕉是三倍体和无菌，新品种无法通过传统繁殖产生。虽然基因编辑为有价值的性状发展提供了重要的希望，但香蕉的基因编辑面临着重大的实施挑战，包括低转换效率和开发非转基因转化方法的要求（由于不可筹集翻译）。我们将开发有效的和非转移的系统型模型，以便于ban bunana inst insteralcitrant inst inst in and bunant inst in in pocore inst inst inst in and bunant inst in and bunant inst in and bunant inst in in pocor bunant inst in nation in not in notal inst in nation in not in pocor bunant int in not nations int in not in posit。