CORE 1 - Animal Phenotyping Core

核心 1 - 动物表型核心

• 批准号: 10512733
• 负责人: PHILIPP E SCHERER
• 金额: $ 16.4万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-07-01 至 2027-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10512733
• 关键词: Animal Model; Animals; Biological Assay; Blood; Blood Chemical Analysis; Body Composition; Calorimetry; Cells; Cholesterol; Collection; Communication; Data; Data Analytics; Disease; Dual-Energy X-Ray Absorptiometry; Enzyme-Linked Immunosorbent Assay; Equipment; Food; Genus Hippocampus; Glucose; Glucose Clamp; Glycolysis; Goals; Guidelines; Hepatocyte; Hormones; Individual; Laboratories; Lipids; Magnetic Resonance Imaging; Measurement; Measures; Metabolic; Metabolism; Mitochondria; Mus; Obesity; Pathogenesis; Phenotype; Physiological; Quality Control; Rattus; Reagent; Research; Research Personnel; Respiration; Services; Speed; Standardization; Technical Expertise; Technology; Testing; Tissues; Triglycerides; Urine; Water consumption; Work; cost effective; cytokine; extracellular; glucose and insulin clamps; glucose tolerance; instrumentation; insulin tolerance; magnetic beads; microCT; nutrition; pre-clinical; prevent; sound; temporal measurement; treadmill

PROJECT SUMMARY/ABSTRACT – Core 1 – Animal Phenotyping/Metabolism Core The services provided in the Animal Phenotyping/Metabolism Core draw upon the expertise of many investigators at UTSW to provide comprehensive analytical capabilities that are on par with the best facilities in the world. We established our Animal Phenotyping/Metabolism Core more than 12 years ago and we have continued to expand our assay capabilities to support investigators with in-depth preclinical capabilities. The availability of standardized assays speeds the pace of our research, facilitates inter-lab comparisons of data, and provides a common platform for the necessary close interactions between investigators of the NORC. Animal models that allow careful characterization of the metabolic effects of obesity and that have an altered communication axis between critical tissues and are vital to understand the pathogenesis of disease. Given the number of phenotypic measurements, the range of target metabolites, and the number of different animal models investigated among the NORC investigators, it is necessary to provide uniform measurements and to have quality control guidelines in place. To facilitate the research of NORC investigators, Core 1 will provide comprehensive, state-of-the-art measurements of multiple metabolic parameters, with measurements involving physiological read outs that include lipid, glucose, and metabokine assays. The technical expertise offered by the Animal Phenotyping/Metabolism Core will bring multiple benefits to NORC investigators, including but not limited to the following:  Centralized Core services that are more cost effective and prevent the duplication of reagents across NORC investigators.  Provides consistency in various assays across laboratories.  Provides consistent quality control measures.  Makes available technically challenging assays, such as hyperinsulinemic-euglycemic clamps, that cannot be established in individual laboratories.  Makes available equipment that is essential for nutrition and obesity research but that is too expensive for individual investigators to purchase (e.g. Seahorse, MRI, metabolic cages, animal CT).  Frees investigators from routine work to focus on the intellectual challenges of the projects.

项目总结/摘要-核心1 -动物表型/代谢核心 动物表型分析/代谢核心提供的服务借鉴了许多 研究人员在UTSW提供全面的分析能力，与最好的设施， 世界我们在12年前建立了动物表型/代谢核心， 继续扩大我们的分析能力，以支持研究人员深入的临床前能力。 标准化检测的可用性加快了我们研究的步伐，促进了实验室间数据的比较， 并为NORC调查人员之间必要的密切互动提供了一个共同平台。 动物模型，允许仔细表征肥胖的代谢影响，并具有改变的 关键组织之间的通讯轴，并了解疾病的发病机制至关重要。鉴于 表型测量的数量、目标代谢物的范围和不同动物模型的数量 在NORC调查人员中进行调查时，有必要提供统一的测量结果， 制定质量控制准则。为了方便NORC研究人员的研究，核心1将提供 多个代谢参数的全面、最先进的测量， 涉及生理学读出，包括脂质、葡萄糖和代谢因子测定。 动物表型分析/代谢中心提供的技术专长将为NORC带来多重好处 研究者，包括但不限于： ·集中的核心服务，更具成本效益，并防止试剂重复使用。 NORC调查员。 在不同实验室的各种检测中提供一致性。 提供一致的质量控制措施。 提供技术上具有挑战性的分析，如高胰岛素-正常血糖钳夹， 不能在个别实验室建立。 * 提供营养和肥胖研究所需的设备，但这些设备太贵 供个体研究者购买（例如Seahorse、MRI、代谢笼、动物CT）。 将调查人员从日常工作中解放出来，专注于项目的智力挑战。