CORE 1 - Animal Phenotyping Core

项目摘要/摘要-核心1--动物表型/代谢核心 动物表型/新陈代谢核心提供的服务利用了许多人的专业知识 UTSW的调查人员提供全面的分析能力，与 整个世界。我们在12年前建立了我们的动物表型/代谢核心，我们已经 继续扩大我们的检测能力，以支持具有深入临床前能力的研究人员。 标准化分析的可获得性加快了我们的研究步伐，促进了实验室间数据的比较， 并为NORC调查人员之间必要的密切互动提供了一个共同平台。 动物模型，允许仔细描述肥胖的代谢影响，并改变了 关键组织之间的通信轴，对了解疾病的发病机制至关重要。给定 表型测量的数量，目标代谢物的范围，以及不同动物模型的数量 在NORC调查人员中进行调查时，有必要提供统一的测量并具有 制定了质量控制指南。为了方便NORC调查人员的研究，核心一号将提供 全面、最先进的多种代谢参数测量，包括测量 涉及生理读数，包括脂肪、葡萄糖和代谢物分析。 动物表型/代谢核心提供的技术专长将为NORC带来多重好处 调查人员，包括但不限于以下人员： 集中化核心服务，更具成本效益，并防止试剂在 北大西洋公约组织调查人员。 在不同实验室的各种检测中提供了一致性。 提供始终如一的质量控制措施。 提供了具有技术挑战性的分析方法，如高胰岛素-正常血糖钳夹， 不能在个别实验室建立。 提供营养和肥胖研究必不可少的设备，但价格太高 供个人研究人员购买(例如，海马、核磁共振、代谢笼、动物CT)。 将调查人员从例行公事中解放出来，专注于这些项目的智力挑战。

PROJECT SUMMARY/ABSTRACT – Core 1 – Animal Phenotyping/Metabolism Core The services provided in the Animal Phenotyping/Metabolism Core draw upon the expertise of many investigators at UTSW to provide comprehensive analytical capabilities that are on par with the best facilities in the world. We established our Animal Phenotyping/Metabolism Core more than 12 years ago and we have continued to expand our assay capabilities to support investigators with in-depth preclinical capabilities. The availability of standardized assays speeds the pace of our research, facilitates inter-lab comparisons of data, and provides a common platform for the necessary close interactions between investigators of the NORC. Animal models that allow careful characterization of the metabolic effects of obesity and that have an altered communication axis between critical tissues and are vital to understand the pathogenesis of disease. Given the number of phenotypic measurements, the range of target metabolites, and the number of different animal models investigated among the NORC investigators, it is necessary to provide uniform measurements and to have quality control guidelines in place. To facilitate the research of NORC investigators, Core 1 will provide comprehensive, state-of-the-art measurements of multiple metabolic parameters, with measurements involving physiological read outs that include lipid, glucose, and metabokine assays. The technical expertise offered by the Animal Phenotyping/Metabolism Core will bring multiple benefits to NORC investigators, including but not limited to the following:  Centralized Core services that are more cost effective and prevent the duplication of reagents across NORC investigators.  Provides consistency in various assays across laboratories.  Provides consistent quality control measures.  Makes available technically challenging assays, such as hyperinsulinemic-euglycemic clamps, that cannot be established in individual laboratories.  Makes available equipment that is essential for nutrition and obesity research but that is too expensive for individual investigators to purchase (e.g. Seahorse, MRI, metabolic cages, animal CT).  Frees investigators from routine work to focus on the intellectual challenges of the projects.