CORE 1 - Animal Phenotyping Core

项目摘要/摘要 – 核心 1 – 动物表型/代谢核心 动物表型/代谢核心提供的服务借鉴了许多人的专业知识 UTSW 的研究人员提供与世界上最好的设施相媲美的全面分析能力 世界。我们在 12 年前建立了动物表型/代谢核心，并且 继续扩大我们的检测能力，以支持具有深入临床前能力的研究人员。 标准化测定的可用性加快了我们的研究步伐，促进了实验室间的数据比较， 并为 NORC 调查人员之间必要的密切互动提供一个共同平台。 动物模型可以仔细表征肥胖的代谢影响，并且改变了 关键组织之间的通讯轴，对于了解疾病的发病机制至关重要。鉴于 表型测量的数量、目标代谢物的范围以及不同动物模型的数量 在 NORC 调查员之间进行调查时，有必要提供统一的测量结果并 质量控制指南到位。为了促进 NORC 研究人员的研究，核心 1 将提供 对多种代谢参数进行全面、最先进的测量 涉及生理读数，包括脂质、葡萄糖和代谢因子测定。 动物表型/代谢核心提供的技术专长将为 NORC 带来多重好处 调查人员，包括但不限于以下人员：  更具成本效益的集中式核心服务，可防止试剂重复 NORC 调查员。  提供跨实验室各种测定的一致性。  提供一致的质量控制措施。  提供技术上具有挑战性的检测，例如高胰岛素-正常血糖钳夹， 不能在个别实验室建立。  提供营养和肥胖研究必需的设备，但价格太昂贵 供个人研究人员购买（例如 Seahorse、MRI、代谢笼、动物 CT）。  将调查人员从日常工作中解放出来，专注于项目的智力挑战。

PROJECT SUMMARY/ABSTRACT – Core 1 – Animal Phenotyping/Metabolism Core The services provided in the Animal Phenotyping/Metabolism Core draw upon the expertise of many investigators at UTSW to provide comprehensive analytical capabilities that are on par with the best facilities in the world. We established our Animal Phenotyping/Metabolism Core more than 12 years ago and we have continued to expand our assay capabilities to support investigators with in-depth preclinical capabilities. The availability of standardized assays speeds the pace of our research, facilitates inter-lab comparisons of data, and provides a common platform for the necessary close interactions between investigators of the NORC. Animal models that allow careful characterization of the metabolic effects of obesity and that have an altered communication axis between critical tissues and are vital to understand the pathogenesis of disease. Given the number of phenotypic measurements, the range of target metabolites, and the number of different animal models investigated among the NORC investigators, it is necessary to provide uniform measurements and to have quality control guidelines in place. To facilitate the research of NORC investigators, Core 1 will provide comprehensive, state-of-the-art measurements of multiple metabolic parameters, with measurements involving physiological read outs that include lipid, glucose, and metabokine assays. The technical expertise offered by the Animal Phenotyping/Metabolism Core will bring multiple benefits to NORC investigators, including but not limited to the following:  Centralized Core services that are more cost effective and prevent the duplication of reagents across NORC investigators.  Provides consistency in various assays across laboratories.  Provides consistent quality control measures.  Makes available technically challenging assays, such as hyperinsulinemic-euglycemic clamps, that cannot be established in individual laboratories.  Makes available equipment that is essential for nutrition and obesity research but that is too expensive for individual investigators to purchase (e.g. Seahorse, MRI, metabolic cages, animal CT).  Frees investigators from routine work to focus on the intellectual challenges of the projects.