Domain-specific inhibition of angiotensin-converting enzyme as a therapeutic strategy for opioid use disorders

血管紧张素转换酶的域特异性抑制作为阿片类药物使用障碍的治疗策略

• 批准号: 10512191
• 负责人: Swati S More
• 金额: $ 177.18万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-07-01 至 2025-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10512191
• 关键词: Address; Affinity; Angiotensin I; Angiotensin II; Angiotensin-Converting Enzyme Inhibitors; Angiotensins; Antihypertensive Agents; Behavioral; Biological Assay; Blood - brain barrier anatomy; Brain; C-terminal; Captopril; Cardiovascular system; Catalytic Domain; Cells; Chemicals; Clinical; Collaborations; DNA Sequence Alteration; Data; Dependence; Dissociation; Dopamine Receptor; Drug Delivery Systems; Drug Exposure; Drug Kinetics; Enkephalins; Enzyme Inhibition; Fentanyl; Genetic; Glutamates; Goals; Hypertension; In Vitro; Infusion procedures; Intravenous; Investigation; Lead; Liquid Chromatography; Measures; Mediating; Metabolic; Methionine Enkephalin; Modification; Motor; Mus; Mutation; N Domain; N-terminal; National Institute of Drug Abuse; Neurons; Nucleus Accumbens; Opioid; Pathway interactions; Peptidyl-Dipeptidase A; Peripheral; Permeability; Pharmaceutical Chemistry; Pharmaceutical Preparations; Pharmacology; Presynaptic Terminals; Principal Investigator; Probability; Prodrugs; Property; Publishing; Recombinant Proteins; Regulation; Research; Rewards; Risk; Role; Schedule; Self Administration; Signal Transduction; Substance Use Disorder; Substrate Specificity; Synaptic Transmission; System; Testing; Therapeutic; Tissues; Transgenic Mice; Wild Type Mouse; addiction; brain tissue; conditioned place preference; drug development; endogenous opioids; experimental study; extracellular; improved; inhibitor; mu opioid receptors; novel; novel therapeutic intervention; novel therapeutics; opioid use disorder; patch clamp; phenylalanylarginine; presynaptic; prevent; side effect; substance use treatment; tandem mass spectrometry; tool; translational potential

ABSTRACT This project combines the mutual expertise of Drs. Patrick Rothwell and Swati More (Principal Investigators) in nucleus accumbens opioid signaling and medicinal chemistry. As part of an ongoing collaboration supported by NIDA (R21 DA050120), we have found that angiotensin-converting enzyme (ACE) has a non-canonical function in the nucleus accumbens: it degrades Met-enkephalin-Arg-Phe (MERF) and thereby regulates endogenous opioid signaling. Conventional ACE inhibitors block the degradation of MERF, leading to an enhancement of endogenous opioid signaling in the nucleus accumbens. This causes a selective reduction of glutamate release onto medium spiny projection neurons that express the Drd1 dopamine receptor (D1-MSNs), which express ACE at a higher level than other neurons. This mechanism of action has great therapeutic potential, as our preliminary data indicate the decrease in excitatory drive to D1-MSNs can diminish the rewarding effects of fentanyl. Previously published enzymatic assays using recombinant protein suggest that MERF is efficiently degraded by the catalytic N-domain of ACE, though this has not been examined in brain tissue. This raises the exciting possibility of a double-dissociation between catalytic domains of ACE that degrade angiotensin (C-domain) and MERF (N-domain). The goal of this project is to independently evaluate the contribution of each ACE catalytic domain to MERF degradation and endogenous opioid signaling in the nucleus accumbens, in order to generate new domain-specific ACE inhibitors with optimized properties for treatment of opioid use disorders. We will use mice as an experimental system to separately manipulate each catalytic domain of ACE, through a combination of complementary genetic and pharmacological manipulations. AIM 1 is to determine which catalytic domain of ACE degrades MERF in nucleus accumbens tissue. We will directly quantify extracellular levels of MERF using liquid chromatography-tandem mass spectrometry, and measure excitatory synaptic transmission using whole-cell patch-clamp recordings from nucleus accumbens neurons. AIM 2 is to determine the behavioral impact of domain-specific ACE inhibition on fentanyl CPP and self-administration. This will build on our preliminary experiments using non-contingent fentanyl exposure (CPP), by incorporating parallel analysis of intravenous fentanyl self-administration on an intermittent access schedule. AIM 3 is to optimize the central activity and drug-like properties of domain-specific ACE inhibitors. We will perform systematic chemical iterations involving (but not limited to) prodrug and drug delivery systems, with the goal of improving permeability across the blood-brain barrier. These experiments should result in the identification and early optimization of compounds that inhibit degradation of MERF by ACE in the brain. This novel mechanism could form the basis of a viable new therapeutic strategy for treating opioid use disorders.

摘要 该项目结合了帕特里克罗斯韦尔博士和Swati More（主要研究者）的共同专业知识， 阿片样物质信号传导和药物化学。作为持续合作的一部分， NIDA（R21 DA 050120）的研究中，我们发现血管紧张素转换酶（ACE）具有非典型的功能 在延髓核中：它降解Met-脑啡肽-Arg-Phe（MERF），从而调节内源性 阿片样物质信号传统的ACE抑制剂阻断MERF的降解，导致MERF的增强。 内源性阿片类物质的信号传导。这导致谷氨酸释放的选择性减少 表达Drd 1多巴胺受体（D1-MSNs）的中型多刺投射神经元， 比其他神经元更高的水平。这种作用机制具有很大的治疗潜力，作为我们的初步研究， 数据表明，对D1-MSN的兴奋性驱动的减少可以减弱芬太尼的奖励作用。 先前发表的使用重组蛋白的酶促测定表明，MERF被以下物质有效降解： ACE的催化N-结构域，尽管这尚未在脑组织中进行研究。这引发了令人兴奋的 降解血管紧张素的ACE催化结构域（C结构域）和 MERF（N-结构域）。该项目的目标是独立评估每种ACE催化剂的贡献， 结构域的MERF降解和内源性阿片样物质的信号传导，以产生 用于治疗阿片类药物使用障碍的具有优化性质的新的结构域特异性ACE抑制剂。我们将使用 小鼠作为实验系统，通过组合 互补的基因和药理学操作。目的1是确定催化结构域 ACE降解髓核组织中的MERF。我们将直接定量细胞外MERF水平 使用液相色谱-串联质谱法，并使用 全细胞膜片钳记录从核神经元。目的2是确定行为 区域特异性ACE抑制对芬太尼CPP和自身给药影响。这将建立在我们的 使用非偶然芬太尼暴露（CPP）的初步实验， 静脉芬太尼自我管理间歇访问时间表。目标3是优化中央 活性和药物样特性的结构域特异性ACE抑制剂。我们将进行系统的化学 涉及（但不限于）前药和药物递送系统的迭代，目的是改善渗透性 穿过血脑屏障这些实验应导致识别和早期优化 抑制脑中ACE降解MERF的化合物。这种新的机制可以形成基础， 一个可行的治疗阿片类药物使用障碍的新治疗策略。

项目成果

Modulation of endogenous opioid signaling by inhibitors of puromycin sensitive aminopeptidase.

嘌呤霉素敏感氨肽酶抑制剂调节内源性阿片类信号传导。

• DOI: 10.1101/2024.04.02.587756
• 发表时间: 2024
• 期刊: bioRxiv : the preprint server for biology
• 作者: Singh,Rohit;Jiang,Rongrong;Williams,Jessica;Dobariya,Prakashkumar;Hanak,Filip;Xie,Jiashu;Rothwell,PatrickE;Vince,Robert;More,SwatiS
• 通讯作者: More,SwatiS