Regulation of Skeletal progenitor cells in Osteogenesis Imperfecta

成骨不全中骨骼祖细胞的调节

• 批准号: 10528208
• 负责人: Brendan Lee
• 金额: $ 66.24万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-07-13 至 2027-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10528208
• 关键词: Affect; Animal Model; Biological; Bone Diseases; Bone Injury; Bone Marrow; Bone Regeneration; Breathing; CCR5 gene; Cell Count; Cell Proliferation; Cell model; Cell physiology; Cells; Characteristics; Clinical; Clinical Trials; Collagen; Craniofacial Abnormalities; Defect; Deformity; Disease; Environment; Extracellular Matrix; Face; Fracture; Gene Expression Profile; Genetic Diseases; Goals; Heterogeneity; Human; Impaired healing; Injury; KDR gene; Label; Ligands; Location; Maintenance; Mastication; Methods; Modeling; Modification; Molecular; Mus; Mutation; Natural regeneration; Oral; Osteoblasts; Osteogenesis Imperfecta; Patients; Periosteal Cell; Periosteum; Population; Procollagen; RANTES; Regulation; Reporter; Series; Severities; Signal Transduction; Site; Source; Surface; Tail; Technology; Testing; Transforming Growth Factor beta; Transplantation; Vascular Endothelial Growth Factors; Wild Type Mouse; Work; base; bone; bone healing; bone repair; bone strength; cell motility; clinically relevant; combinatorial; cortical bone; craniofacial; craniofacial bone; craniofacial disorder; fracture risk; improved; in vivo; insight; intravital imaging; long bone; migration; mouse model; neutralizing antibody; novel; osteoprogenitor cell; postnatal; progenitor; receptor; restoration; selective expression; single cell analysis; single-cell RNA sequencing; skeletal; skeletal disorder; skeletal stem cell; stem; stem cell function; stem cells; success

PROJECT SUMMARY Osteogenesis Imperfecta (OI) is a congenital bone and oral-facial disorder that mainly affects bone with less well characterized alterations in bone healing and repair. Like other skeletal diseases, at least some of the progressive bone and craniofacial defects in OI patients have been attributed to changes in the populations and functions of their stem/progenitor cells. In general, stem cells require a specialized environment for their maintenance and function. Nevertheless, how normal or defective structural components of collagen regulate skeletal stem/progenitor cells (SSPCs) is essentially unknown. Therefore, the goal of this proposal is to define the in vivo characteristics and function of skeletal stem/progenitor cells in OI and to understand how an abnormal OI extracellular matrix alters these stem/progenitor cells in the context of bone regeneration and repair. We previously showed that the Mx1Cre and αSMAGFP combination can selectively label skeletal stem cells in the periosteum and that these Mx1ÈαSMAGFPÈ periosteal cells are long-term repopulating stem cell subsets responsible for lifelong regeneration of periosteal osteoblasts and bone repair. Moreover, our preliminary study revealed that these periosteal SSPCs are Prx1GFP positive and selectively express KDR (VEGFR2). Human primary periosteal cells also express KDR with multi-lineage differentiation potentials. Notably, we found that these KDRÈ periosteal progenitor cells are significantly decreased in OI bones. Hence, we hypothesize that the abnormal OI matrix deregulates the number and function of periosteal and bone marrow SSPCs and the OI- associated molecular changes in stem/progenitor cells are critical for the progressive deformity and delayed or defective healing of bones. By using a series of OI animal models and SSPC reporter mice, in which we can differentially label periosteal and bone marrow SSPC subsets, in combination with intravital imaging and the latest single-cell RNA-sequencing technology, we plan to pursue the following specific aims. In aim 1, we will define the in vivo characteristics and function of periosteal and bone marrow SSPCs in clinically relevant OI mouse models. In aim 2, we will define key OI matrix factors that regulate SSPC function and improve both craniofacial and long bone healing. Upon completion of this work, we will achieve new biological insights into a better understanding of the molecular and cellular mechanisms that differentially regulate SSPCs under OI pathophysiological conditions.

项目总结 成骨不全(OI)是一种先天性骨骼和口腔-面部疾病，主要影响骨骼，但功能不佳 以骨骼愈合和修复中的变化为特征。像其他骨骼疾病一样，至少有一些 OI患者的进行性骨和颅面缺陷被归因于人口和 干细胞/祖细胞的功能。一般说来，干细胞需要一个专门的环境来实现它们的 维护和使用。然而，胶原的正常或缺陷的结构成分是如何调节的 骨骼干细胞/祖细胞(SSPC)基本上是未知的。因此，这项提案的目标是明确 目的：了解OI中骨骼干/祖细胞的体内特性和功能。 在骨再生的背景下，异常的OI细胞外基质改变这些干/祖细胞 并进行修复。我们之前已经证明，Mx1Cre和αSMAGFP组合可以选择性地标记骨骼干细胞 这些Mx1？α？SMAGFP？骨膜细胞是长期再生干细胞。 负责骨膜成骨细胞的终生再生和骨修复的亚群。而且，我们初步的 研究表明，这些骨膜SSPC呈Prx1GFP阳性，选择性表达KDR(VEGFR2)。人类 原代骨膜细胞也表达KDR，具有多向分化潜能。值得注意的是，我们发现 这些KDRé骨膜祖细胞在OI骨中显著减少。因此，我们假设 异常的OI基质使骨膜和骨髓SSPC的数量和功能异常，并使OI- 干细胞/祖细胞的相关分子变化是进行性畸形和延迟或 骨头愈合有缺陷。通过使用一系列OI动物模型和SSPC报告小鼠，我们可以 差异标记骨膜和骨髓SSPC亚群，结合活体成像和 最新的单细胞RNA测序技术，我们计划追求以下具体目标。在目标1中，我们将 明确临床相关OI中骨膜和骨髓SSPC的体内特征和功能 老鼠模型。在目标2中，我们将定义调节SSPC功能并改善两者的关键OI矩阵因素 颅面和长骨愈合。在这项工作完成后，我们将对一种 更好地理解OI下SSPC差异调控的分子和细胞机制 病理生理条件。