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Modernization of a Shared-use Gnotobiotic Animal Core

共享使用知生动物核心的现代化

基本信息

批准号：
10532456
负责人：
JISUN PAIK
金额：
$ 7.19万
依托单位：
UNIVERSITY OF WASHINGTON
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-08-01 至 2023-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10532456
关键词：
Address Air Air Pressure Animal Behavior Animal Experimentation Animal Husbandry Animal Welfare Animals Area Avidity Breeding Budgets Canis familiaris Caring Communities Complex Core Facility Data Disease Disease Progression Electronic Mail Environment Environmental Monitoring Environmental Risk Factor Equipment Faculty Film Floods Food Foundations Funding Funding Agency Funding Opportunities Genetic Germ-Free Gnotobiotic Goals Grant Health Health Sciences Health care facility House mice Housing Human Human Resources Humidity In Vitro Individual Injections Institution Light Maintenance Medicine Methods Mission Modernization Monitor Mus Neoplasm Transplantation Noise Pilot Projects Privatization Process Public Health Publications Reproducibility Research Research Personnel Research Support Resources Role Schedule Side Specific qualifier value Sterility System Techniques Technology Telephone Temperature Time Time Management Training Translations Treatment Efficacy Universities Visual Washington Water air monitoring animal care animal facility comparative digital environmental change experimental study flexibility germ free condition high efficiency particulate air filter improved in vivo member microbiome microbiome research mouse model risk minimization user-friendly vibration welfare

项目摘要

PROJECT SUMMARY The Gnotobiotic Animal Core (GNAC) is a specialized mouse care facility that is shared by investigators within and outside of the University of Washington (UW). It is located in the specific pathogen free (SPF) vivarium in the Health Sciences Buildings at UW and occupies ~1750 sq ft consisting of four separate rooms. It is managed by two faculty members and highly trained GNAC Staff in the Department of Comparative Medicine. The GNAC is a complete research support facility: We create and care for gnotobiotic and germ-free mice housed in the core and provide technical support for diverse gnotobiotic research needs. A particular strength of the core is that new investigators can conduct small pilot projects by using the ready-to-go expertise of GNAC staff even if they do not have the extensive training needed for such studies. The GNAC has been operational for over seven years, and during this period, more than forty investigators from fifteen different research groups have used the GNAC. Over twenty publications have been generated with support from twenty-three federal grants and twelve private foundation/institutional grants. GNAC utilizes two housing systems: Flexible-film isolators are used for breeding and maintenance of various strains of GF mice, and isopositive cages are used for mice during experiments. The isopositive cages are highly specialized, air-tight, HEPA filtered, bioexclusion cages (ISOcage P caging manufactured by Tecniplast). When paired with using a biosafety cabinet and sterile technique, the isopositive cages offer a more user-friendly alternative to isolators for investigators to maintain and manipulate gnotobiotic mice. The goal of this application is to retrofit existing ISOcage P cages with a Digital Ventilated Cage Rack Environment Monitoring (DVC REM) system. The DVC REM system will monitor cage- and rack- level environments as well as global animal activity within each cage continuously. This modernization will greatly enhance several experimental and husbandry-related aspects of gnotobiotic research using mice: 1) improved experimental rigor, reproducibility and translation of experiments, resulting in reduced number of research animals needed to address complex research questions; 2) improved animal welfare by providing 24/7 monitoring that will alert investigators to problems with animal health or the caging environment; and 3) improved time management for GNAC staff during animal husbandry practices by streamlining scheduling of animal care- related tasks and preparing and rotating supplies. Successful completion of our proposed plan will broadly benefit all investigators using the facility by helping to maximize the use of limited resources and to improve reproducibility in animal research by providing unprecedented continuous monitoring of environment and animal activities.

项目概要 Gnotobiotic Animal Core (GNAC) 是一个专门的小鼠护理设施，由内部研究人员共享以及华盛顿大学 (UW) 之外。它位于无特定病原体 (SPF) 动物园中华盛顿大学健康科学大楼，占地约 1750 平方英尺，由四个独立的房间组成。是被管理的由比较医学系的两名教职人员和训练有素的 GNAC 工作人员负责。 GNAC 是一个完整的研究支持设施：我们创造并照顾饲养在环境中的无菌和无菌小鼠核心并为多样化的无菌研究需求提供技术支持。核心的特殊强度是新的调查人员可以利用 GNAC 工作人员随时可用的专业知识来开展小型试点项目，即使他们没有接受此类研究所需的广泛培训。 GNAC 已运行七年多多年来，在此期间，来自十五个不同研究小组的四十多名研究人员使用了 GNAC。在 23 项联邦拨款和 12 项联邦拨款的支持下，已出版了 20 多种出版物。私人基金会/机构赠款。 GNAC 使用两种外壳系统：柔性薄膜隔离器用于各种品系GF小鼠的饲养和饲养，期间使用同阳性笼养小鼠实验。等阳性笼是高度专业化、气密、HEPA 过滤、生物排斥笼 (ISOcage P 笼由 Tecniplast 制造）。当与使用生物安全柜和无菌技术配合使用时，等阳性笼提供了一种更用户友好的隔离器替代方案，供研究人员维护和操作限生小鼠。此应用的目标是使用数字通风改造现有的 ISOcage P 保持架笼架环境监测（DVC REM）系统。 DVC REM 系统将监控笼式和机架式水平环境以及每个笼子内的全球动物活动不断。这种现代化将大大加强使用小鼠进行的限生素研究的几个实验和饲养相关方面：1）改进实验的严谨性、可重复性和实验转化，导致研究数量减少需要解决复杂研究问题的动物； 2) 通过提供 24/7 改善动物福利进行监测，提醒调查人员注意动物健康或笼养环境的问题； 3）改进通过简化动物护理的日程安排，对 GNAC 工作人员在畜牧业实践中的时间进行管理相关任务以及准备和轮换物资。成功完成我们提出的计划将广泛通过帮助最大限度地利用有限资源并改进使用该设施的所有调查人员受益通过提供前所未有的环境和动物连续监测，提高动物研究的可重复性活动。