Modernization of a Shared-use Gnotobiotic Animal Core
Modernization of a Shared-use Gnotobiotic Animal Core
批准号:
10532456
负责人:
JISUN PAIK
金额:
$7.19万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2022
资助国家:
美国
项目状态:
已结题
起止时间:
2022-08-01 至 2023-07-31
关键词:
AddressAirAir PressureAnimal BehaviorAnimal ExperimentationAnimal HusbandryAnimal WelfareAnimalsAreaAvidityBreedingBudgetsCanis familiarisCaringCommunitiesComplexCore FacilityDataDiseaseDisease ProgressionElectronic MailEnvironmentEnvironmental MonitoringEnvironmental Risk FactorEquipmentFacultyFilmFloodsFoodFoundationsFundingFunding AgencyFunding OpportunitiesGeneticGerm-FreeGnotobioticGoalsGrantHealthHealth SciencesHealth care facilityHouse miceHousingHumanHuman ResourcesHumidityIn VitroIndividualInjectionsInstitutionLightMaintenanceMedicineMethodsMissionModernizationMonitorMusNeoplasm TransplantationNoisePilot ProjectsPrivatizationProcessPublic HealthPublicationsReproducibilityResearchResearch PersonnelResearch SupportResourcesRoleScheduleSideSpecific qualifier valueSterilitySystemTechniquesTechnologyTelephoneTemperatureTimeTime ManagementTrainingTranslationsTreatment EfficacyUniversitiesVisualWashingtonWaterair monitoringanimal careanimal facilitycomparativedigitalenvironmental changeexperimental studyflexibilitygerm free conditionhigh efficiency particulate air filterimprovedin vivomembermicrobiomemicrobiome researchmouse modelrisk minimizationuser-friendlyvibrationwelfare
中文摘要
项目总结
英文摘要
PROJECT SUMMARY
The Gnotobiotic Animal Core (GNAC) is a specialized mouse care facility that is shared by investigators within
and outside of the University of Washington (UW). It is located in the specific pathogen free (SPF) vivarium in
the Health Sciences Buildings at UW and occupies ~1750 sq ft consisting of four separate rooms. It is managed
by two faculty members and highly trained GNAC Staff in the Department of Comparative Medicine. The GNAC
is a complete research support facility: We create and care for gnotobiotic and germ-free mice housed in the
core and provide technical support for diverse gnotobiotic research needs. A particular strength of the core is
that new investigators can conduct small pilot projects by using the ready-to-go expertise of GNAC staff even if
they do not have the extensive training needed for such studies. The GNAC has been operational for over seven
years, and during this period, more than forty investigators from fifteen different research groups have used the
GNAC. Over twenty publications have been generated with support from twenty-three federal grants and twelve
private foundation/institutional grants. GNAC utilizes two housing systems: Flexible-film isolators are used for
breeding and maintenance of various strains of GF mice, and isopositive cages are used for mice during
experiments. The isopositive cages are highly specialized, air-tight, HEPA filtered, bioexclusion cages (ISOcage
P caging manufactured by Tecniplast). When paired with using a biosafety cabinet and sterile technique, the
isopositive cages offer a more user-friendly alternative to isolators for investigators to maintain and manipulate
gnotobiotic mice. The goal of this application is to retrofit existing ISOcage P cages with a Digital Ventilated
Cage Rack Environment Monitoring (DVC REM) system. The DVC REM system will monitor cage- and rack-
level environments as well as global animal activity within each cage continuously. This modernization will greatly
enhance several experimental and husbandry-related aspects of gnotobiotic research using mice: 1) improved
experimental rigor, reproducibility and translation of experiments, resulting in reduced number of research
animals needed to address complex research questions; 2) improved animal welfare by providing 24/7
monitoring that will alert investigators to problems with animal health or the caging environment; and 3) improved
time management for GNAC staff during animal husbandry practices by streamlining scheduling of animal care-
related tasks and preparing and rotating supplies. Successful completion of our proposed plan will broadly
benefit all investigators using the facility by helping to maximize the use of limited resources and to improve
reproducibility in animal research by providing unprecedented continuous monitoring of environment and animal
activities.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Validation of ALDH1A1 as a target for the treatment of obesity: effects of ALDH1A1 inhibition on energy metabolism
-
批准号:10681349
-
项目类别:
-
资助金额:$39.41万
-
财政年份:2022
-
负责人:JISUN PAIK
-
依托单位:
Validation of ALDH1A1 as a target for the treatment of obesity: effects of ALDH1A1 inhibition on energy metabolism
-
批准号:10443471
-
项目类别:
-
资助金额:$40.0万
-
财政年份:2022
-
负责人:JISUN PAIK
-
依托单位:
Inhibition of ALDH1A1 for the treatment of obesity
-
批准号:9517371
-
项目类别:
-
资助金额:$31.1万
-
财政年份:2017
-
负责人:JISUN PAIK
-
依托单位:
Regulation of vitamin A synthesis from carotenoids
-
批准号:6885742
-
项目类别:
-
资助金额:$20.92万
-
财政年份:2004
-
负责人:JISUN PAIK
-
依托单位:
Regulation of vitamin A synthesis from carotenoids
-
批准号:7025054
-
项目类别:
-
资助金额:$20.43万
-
财政年份:2004
-
负责人:JISUN PAIK
-
依托单位:
Regulation of vitamin A synthesis from carotenoids
-
批准号:6704470
-
项目类别:
-
资助金额:$26.61万
-
财政年份:2004
-
负责人:JISUN PAIK
-
依托单位:
Regulation of vitamin A synthesis from carotenoids
-
批准号:7191753
-
项目类别:
-
资助金额:$19.84万
-
财政年份:2004
-
负责人:JISUN PAIK
-
依托单位:
国内基金
海外基金
湍流和化学交互作用对H2-Air-H2O微混燃烧中NO生成的影响研究
-
批准号:51976048
-
项目类别:面上项目
-
资助金额:61.0万元
-
批准年份:2019
-
负责人:邱朋华
-
依托单位: