Data Analysis, Integration, and Sharing Core
Data Analysis, Integration, and Sharing Core
批准号:
10531006
负责人:
Robert michael Angelo
金额:
$46.44万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2022
资助国家:
美国
项目状态:
未结题
起止时间:
2022-08-01 至 2026-07-31
关键词:
AgeAlgorithmsAnatomyAtlasesBiological AssayBlood VesselsBone MarrowCell CommunicationCell Differentiation processCellsCensusesClassificationCollaborationsComplexDataData AnalysesErythroidFAIR principlesGenderGene set enrichment analysisGenesGenetic TranscriptionHematopoiesisHematopoieticHistologicHumanHuman BioMolecular Atlas ProgramImageLengthLinkLocationLymphaticLymphoidManualsMetadataMitoticModalityMorphologyMyelogenousNeighborhoodsOrganPatientsPeer ReviewPhagocytesPhenotypePolysaccharidesPopulationPrincipal Component AnalysisQuality ControlRaceResolutionSamplingShapesSpatial DistributionSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationStructureTissue atlasTissue imagingTissuesWorkbasebonecell typecohortcomputerized data processingcomputerized toolsdata pipelinedata qualitydata sharingdeep learning algorithmdemographicsdifferential expressionfeature extractionhuman tissueinteroperabilitylarge datasetsmultimodalitynano-stringprogenitorprogramsprotein expressionstemtissue mappingtooltranscriptometranscriptomics
中文摘要
项目总结-数据分析核心
英文摘要
Project Summary - Data Analysis Core
In close collaboration with the bone marrow Tissue Mapping Core Organ Specific Project (OSP), the data
analysis core (DAC) will oversee sample metadata curation, low level data processing and quality control, spatial
feature extraction, cross-assay integration, tissue atlas construction, and HIVE data delivery. We will leverage
vetted, peer-reviewed data pipelines for scalable processing and analysis of multiplexed tissue imaging data
previously that adhere to FAIR data sharing standards and have been constructed to prioritize interoperability
and transparency. The DAC will use these tools to construct a multiscale, multimodal atlas of human bone
marrow that is contextualized by age, gender, race, and anatomic site. Highly multiplexed subcellular imaging
using MIBI-TOF will provide a comprehensive census of bone marrow structure, cell types, and functional states
that will be coregistered with de novo spatial glycan imaging and tissue transcriptomics performed on adjacent
serial sections using MALDI and Nanostring DSP, respectively. Whole cell segmentation together with single
channel protein expression data will be leveraged for cell clustering and annotation in high resolution MIBI-TOF
images. Meanwhile, pixel-based approaches will be employed to identify complex phenotypic features in a cell-
agnostic manner that can be readily deployed on both MIBI-TOF and MALDI data. These data will be
interrogated with respect to cell or pixel-based features to identify composite tissue neighborhoods consisting of
recurring combinations of either feature type. Lastly, image co-registration across each modality will permit the
spatial distributions of cell subsets characterized by MIBI to be correlated with local glycan composition and
transcriptional programs.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Multimodal histologic atlas of human bone marrow
-
批准号:10531005
-
项目类别:
-
资助金额:$150.0万
-
财政年份:2022
-
负责人:Robert michael Angelo
-
依托单位:
Multimodal histologic atlas of human bone marrow
-
批准号:10924351
-
项目类别:
-
资助金额:$5.0万
-
财政年份:2022
-
负责人:Robert michael Angelo
-
依托单位:
Multimodal histologic atlas of human bone marrow
-
批准号:10673893
-
项目类别:
-
资助金额:$174.52万
-
财政年份:2022
-
负责人:Robert michael Angelo
-
依托单位:
Project 2: Cellular topography and function of the breast cancer tissue microenvironment
-
批准号:10704687
-
项目类别:
-
资助金额:$34.15万
-
财政年份:2021
-
负责人:Robert michael Angelo
-
依托单位:
Project 2: Cellular topography and function of the breast cancer tissue microenvironment
-
批准号:10272390
-
项目类别:
-
资助金额:$26.33万
-
财政年份:2021
-
负责人:Robert michael Angelo
-
依托单位:
A robust platform for multiplexed, subcellular proteomic imaging in human tissue
-
批准号:9894465
-
项目类别:
-
资助金额:$59.0万
-
财政年份:2019
-
负责人:Robert michael Angelo
-
依托单位:
A robust platform for multiplexed, subcellular proteomic imaging in human tissue
-
批准号:10247827
-
项目类别:
-
资助金额:$54.7万
-
财政年份:2019
-
负责人:Robert michael Angelo
-
依托单位:
MIRIAD - Multiplexed Imaging of Resilience In Alzheimers Disease
-
批准号:9974453
-
项目类别:
-
资助金额:$87.92万
-
财政年份:2017
-
负责人:Robert michael Angelo
-
依托单位:
The Phenotypic Landscape of Cognitive Decline as Revealed by Next-Generation Multiplexed Ion Beam Imaging
-
批准号:9910356
-
项目类别:
-
资助金额:$51.42万
-
财政年份:2017
-
负责人:Robert michael Angelo
-
依托单位:
MIRIAD - Multiplexed Imaging of Resilience In Alzheimers Disease
-
批准号:9439172
-
项目类别:
-
资助金额:$93.48万
-
财政年份:2017
-
负责人:Robert michael Angelo
-
依托单位:
MIRIAD - Multiplexed Imaging of Resilience In Alzheimers Disease
-
批准号:10212937
-
项目类别:
-
资助金额:$86.5万
-
财政年份:2017
-
负责人:Robert michael Angelo
-
依托单位:
MIRIAD - Multiplexed Imaging of Resilience In Alzheimers Disease
-
批准号:9756278
-
项目类别:
-
资助金额:$89.33万
-
财政年份:2017
-
负责人:Robert michael Angelo
-
依托单位:
Predictive signatures in breast cancer using multiplexed ion beam imaging
-
批准号:9341982
-
项目类别:
-
资助金额:$40.13万
-
财政年份:2014
-
负责人:Robert michael Angelo
-
依托单位:
Predictive signatures in breast cancer using multiplexed ion beam imaging
-
批准号:8795909
-
项目类别:
-
资助金额:$40.13万
-
财政年份:2014
-
负责人:Robert michael Angelo
-
依托单位:
海外基金