Modernization/operational improvements to basic centrifugation infrastructure of the Shared Instruments Pool in the Department of Biochemistry University of Colorado Boulder

科罗拉多大学博尔德分校生物化学系共享仪器池基本离心基础设施的现代化/运营改进

• 批准号: 10533249
• 负责人: Annette H Erbse
• 金额: $ 14.25万
• 依托单位: UNIVERSITY OF COLORADO
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-07-15 至 2023-07-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10533249
• 关键词: Acceleration; Accountability; Age; Basic Science; Biochemistry; Biomedical Engineering; Biomedical Research; Biotechnology; Cells; Centrifugation; Charge; Chemical Engineering; Colorado; Computers; Core Facility; Custom; DNA; Data; Diagnosis; Equipment; Equus caballus; Faculty; Floor; Free Will; Funding; Future; G Forces; Grant; Graph; Harvest; Individual; Infrastructure; Institutes; Interruption; Location; Manuals; Methods; Minor; Modeling; Modernization; Monitor; Nature; Organelles; Paper; Preparation; Procedures; Productivity; Proteins; RNA; Reaction Time; Reproducibility; Research; Research Infrastructure; Research Personnel; Research Project Grants; Resources; Role; Running; Safety; Sampling; Speed; Students; System; Time; Torque; Touch sensation; United States National Institutes of Health; Universities; Work; aged; base; cost; empowered; flexibility; handheld mobile device; improved; instrument; instrumentation; negative affect; programs; repaired; risk minimization; success

Project Summary/Abstract The Shared Instrument Pool (SIP) core facility in the Department of Biochemistry at CU-Boulder provides a combination of cutting-edge instrumentation and well-maintained basic research instrumentation (e.g., high- speed centrifuges), the latter of which are essential for biomedical research programs on campus. SIP widely and positively impacts many biomedical research programs at CU-Boulder, but this requires acquiring and maintaining modern, state-of-the-art instrumentation. Doing so enables streamlined workflows, better user support, higher throughput and productivity, and improves sample consistency, reproducibility, and data rigor. SIP currently maintains 5 high-speed, high-volume floor centrifuges and multiple different rotors, which are used by 12 major and many minor user labs spread over multiple departments. Many of the research projects that utilize the high-speed centrifuged are funded by grants from NIH, and nearly all are biomedical in nature. The current high-speed centrifuges are functional, but are all over 25 years old, which makes them difficult to maintain and they lack the up-to-data capabilities found on modern centrifuges. This is beginning to cripple efficient, safe, easy-to-support high throughput workflows in SIP. Therefore, funds are requested to modernize our centrifugation infrastructure with three Avanti JXN26 high-speed, large-volume centrifuges to replace three of our outdated models, and three rotors (two J-LITE® JLA-12.500 fixed-angle rotors and one JA30.50Ti fixed angle rotor). The proposal has a single Specific Aim, “Streamline and improve high-speed centrifugation in the Shared Instruments Pool”, and three Sub-Aims. 1.1 Improve user support, reproducibility, and centrifugation safety. The current centrifuges lack onboard computers, remote connectivity, the ability to save user profiles and customized methods, real-time run graphing, and simultaneous display of set and actual run conditions, all of which would be provided by the new high-speed centrifuges. These functionalities will minimize the potential for mistakes, run interruptions, sample loss, wasted time, and centrifuge damage and downtime. Moreover, the new centrifuges will be networked for method transfer and the MobileFuge App will allow users and SIP staff to check on runs in real time from anywhere and to receive messages concerning the runs. Finally, the new centrifuges and new rotors will detect any mismatch between the rotor and the chosen program and can stop a run to avoid damage. 1.2 Improve throughput. The new centrifuges will minimize the time needed to set up runs, and enable shorter runtimes by accessing higher g-forces and better k values, which together will decrease the time needed to achieve sample pelleting and allow higher throughput. 1.3 Minimize the administrative effort connected to centrifugation. Currently centrifuge usage is tracked manually via paper logs. The new centrifuges will store detailed usage data automatically, which will make usage tracking effortless and usage-based charging more accurate. This will free staff time for more important user and instrument support.

项目摘要/摘要 科罗拉多大学博尔德分校生物化学系的共享仪器库(SIP)核心设施提供了 尖端仪器和维护良好的基础研究仪器的结合(例如， 高速离心机)，后者对于校园生物医学研究项目是必不可少的。广泛的SIP 并对CU-Boulder的许多生物医学研究项目产生了积极影响，但这需要获得和 维护现代化、最先进的仪器设备。这样做可以简化工作流程、提高用户体验 支持、更高的吞吐量和生产率，并提高了样品的一致性、再现性和数据的严密性。 Sip目前维护着5台高速、大容量的地板离心机和多个不同的转子，它们被使用 由12个主要用户和许多次要用户组成的实验室分布在多个部门。许多研究项目 使用高速离心机是由美国国立卫生研究院拨款资助的，几乎所有的都是生物医学性质的。这个 目前的高速离心机是可以工作的，但都是25年以上的，这使得它们很难维护 而且它们缺乏现代离心机上的最新数据能力。这开始削弱高效、安全、 在SIP中易于支持的高吞吐量工作流。因此，我们需要资金来实现我们的现代化 离心机基础设施用三台Avanti JXN26高速大容量离心机取代三台 我们过时的型号，和三个转子(两个J-Lite®JLA-12.500固定角度转子和一个JA30.50Ti固定转子 角度转子)。该提案只有一个明确的目标，即“简化和改进高速离心机 共享工具库“和三个分目标。1.1提高用户支持、再现性和 离心机的安全性。目前的离心机缺乏机载计算机、远程连接、节省成本的能力 用户配置文件和定制方法、实时运行图以及设置和实际运行的同时显示 所有这些条件都将由新的高速离心机提供。这些功能将最小化 可能出现错误、运行中断、样品丢失、浪费时间以及离心机损坏和停机。 此外，新的离心机将联网进行方法转移，MobileFuge应用程序将允许用户 和SIP工作人员从任何地方实时检查运行并接收有关运行的消息。最后， 新的离心机和新的转子将检测到转子和所选程序之间的任何不匹配，并可以 停止奔跑以避免损坏。1.2提高吞吐量。新的离心机将最大限度地缩短设置 向上运行，并通过访问更高的重力和更好的k值来实现更短的运行时间，这两个值加在一起将 减少实现样品制粒所需的时间，并允许更高的吞吐量。1.3最小化 与离心法有关的行政工作。目前，离心机的使用情况是通过纸质日志手动跟踪的。 新的离心机将自动存储详细的使用数据，这将使使用跟踪毫不费力和 基于使用情况的收费更加准确。这将使员工腾出时间用于更重要的用户和仪器支持。