Modernization/operational improvements to basic centrifugation infrastructure of the Shared Instruments Pool in the Department of Biochemistry University of Colorado Boulder

项目概要/摘要 CU-Boulder生物化学系的共享仪器池（SIP）核心设施提供了一个 尖端仪器和维护良好的基础研究仪器的组合（例如，表示“高”之义 高速离心机），后者对于校园内的生物医学研究项目至关重要。SIP广泛 并积极影响许多生物医学研究计划在CU博尔德，但这需要收购， 保持现代化的最先进的仪器这样做可以简化工作流程，提高用户体验 支持，更高的通量和生产率，并提高样品的一致性，再现性和数据的严谨性。 SIP目前拥有5台高速、大容量的落地离心机和多种不同的转子， 分布在多个部门的12个主要和许多次要用户实验室。许多研究项目， 利用高速离心的赠款都是由NIH资助的，几乎所有的研究都是生物医学性质的。的 目前的高速离心机都能正常工作，但都已经使用了25年以上，这使得它们很难维护 他们缺乏现代离心机的最新数据能力。这开始削弱高效，安全， 易于支持SIP中的高吞吐量工作流。因此，要求提供资金，使我们的 三台Avanti JXN 26高速大容量离心机取代三台 我们过时的型号，和三个转子（两个J-LITE® JLA-12.500固定角度转子和一个JA30.50Ti固定角度转子） 角转子）。该提案有一个单一的具体目标，“简化和改善高速离心， 共享仪器池”，以及三个子目标。1.1提高用户支持、再现性和 离心安全目前的离心机缺乏机载计算机，远程连接，保存能力， 用户配置文件和自定义方法、实时运行图表以及设定和实际运行的同步显示 所有这些都将由新的高速离心机提供。这些功能将最小化 错误、运行中断、样品损失、浪费时间以及离心机损坏和停机的可能性。 此外，新的离心机将联网进行方法转移，MobileFuge App将允许用户 和SIP工作人员从任何地方以真实的时间检查运行并接收关于运行的消息。最后， 新的离心机和新的转子将检测转子和所选程序之间的任何不匹配， 停止运行以避免损坏。1.2提高吞吐量。新的离心机将最大限度地减少凝固所需的时间， 运行，并通过访问更高的重力和更好的k值来缩短运行时间，这将 减少实现样品造粒所需的时间并允许更高的通量。1.3最小化 与离心分离有关的行政工作。目前，离心机使用情况通过纸质日志进行手动跟踪。 新的离心机将自动存储详细的使用数据，这将使使用跟踪毫不费力， 基于使用的计费更准确。这将使工作人员有更多的时间来为更重要的用户和仪器提供支持。

Project Summary/Abstract The Shared Instrument Pool (SIP) core facility in the Department of Biochemistry at CU-Boulder provides a combination of cutting-edge instrumentation and well-maintained basic research instrumentation (e.g., high- speed centrifuges), the latter of which are essential for biomedical research programs on campus. SIP widely and positively impacts many biomedical research programs at CU-Boulder, but this requires acquiring and maintaining modern, state-of-the-art instrumentation. Doing so enables streamlined workflows, better user support, higher throughput and productivity, and improves sample consistency, reproducibility, and data rigor. SIP currently maintains 5 high-speed, high-volume floor centrifuges and multiple different rotors, which are used by 12 major and many minor user labs spread over multiple departments. Many of the research projects that utilize the high-speed centrifuged are funded by grants from NIH, and nearly all are biomedical in nature. The current high-speed centrifuges are functional, but are all over 25 years old, which makes them difficult to maintain and they lack the up-to-data capabilities found on modern centrifuges. This is beginning to cripple efficient, safe, easy-to-support high throughput workflows in SIP. Therefore, funds are requested to modernize our centrifugation infrastructure with three Avanti JXN26 high-speed, large-volume centrifuges to replace three of our outdated models, and three rotors (two J-LITE® JLA-12.500 fixed-angle rotors and one JA30.50Ti fixed angle rotor). The proposal has a single Specific Aim, “Streamline and improve high-speed centrifugation in the Shared Instruments Pool”, and three Sub-Aims. 1.1 Improve user support, reproducibility, and centrifugation safety. The current centrifuges lack onboard computers, remote connectivity, the ability to save user profiles and customized methods, real-time run graphing, and simultaneous display of set and actual run conditions, all of which would be provided by the new high-speed centrifuges. These functionalities will minimize the potential for mistakes, run interruptions, sample loss, wasted time, and centrifuge damage and downtime. Moreover, the new centrifuges will be networked for method transfer and the MobileFuge App will allow users and SIP staff to check on runs in real time from anywhere and to receive messages concerning the runs. Finally, the new centrifuges and new rotors will detect any mismatch between the rotor and the chosen program and can stop a run to avoid damage. 1.2 Improve throughput. The new centrifuges will minimize the time needed to set up runs, and enable shorter runtimes by accessing higher g-forces and better k values, which together will decrease the time needed to achieve sample pelleting and allow higher throughput. 1.3 Minimize the administrative effort connected to centrifugation. Currently centrifuge usage is tracked manually via paper logs. The new centrifuges will store detailed usage data automatically, which will make usage tracking effortless and usage-based charging more accurate. This will free staff time for more important user and instrument support.