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AAV capsids and their cellular interactions

AAV 衣壳及其细胞相互作用

基本信息

批准号：
10538552
负责人：
ROBERT MCKENNA
金额：
$ 41.08万
依托单位：
UNIVERSITY OF FLORIDA
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-09-17 至 2024-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10538552
关键词：
Animal Model Antibodies Antibody Binding Sites Binding Binding Sites Biological Assay Biological Products Birds Brain Capsid Cattle Cell Surface Receptors Cells Chiroptera Clinical Complex Cryoelectron Microscopy Data Defect Dependovirus Disease Dot Immunoblotting Eligibility Determination Engineering Epitope Mapping Epitopes Evaluation Exposure to Eye Funding Gene Delivery Gene Expression General Population Genetic Diseases Glycogen storage disease type II Gorilla gorilla Human Immune Immune response Immunity Immunoglobulin G In Vitro Infection Intravenous Immunoglobulins Liver Lung Mammalian Cell Maps Mediating Modeling Modification Molecular Monoclonal Antibodies Mus Organ Outcome Parvoviridae Parvovirus Patients Pattern Phase Polysaccharides Population Primates Property Proteins Resolution Seroprevalences Serotyping Serum Serum Proteins Single Stranded DNA Virus Sinus Site Skeletal Muscle Snakes Structure Surface System Testing Therapeutic Tissues Treatment Efficacy Viral Viral Packaging Viral Vector Virus adeno-associated viral vector antibody engineering clinical efficacy clinically relevant cohort comparative delivery vehicle gene delivery system gene therapy glycosylation human tissue image reconstruction improved in vivo member murine monoclonal antibody neutralizing antibody nonhuman primate novel preclinical trial protein expression receptor response success therapeutic gene tissue tropism transduction efficiency transgene expression vector viral gene delivery

项目摘要

The Adeno-associated viruses (AAVs) are ssDNA packaging viruses belonging to the Dependoparvovirus genus of the Parvoviridae. Gene delivery systems based on the AAVs recently entered an exciting phase with the FDA approval of Luxturna, an AAV serotype 2 (AAV2)-based gene therapy for treating a monogenetic defect in the eye. However, the success of Luxturna was ushered by the fact that the eye is an immune privileged organ and direct administration avoids pre-existing host immunity. This remains a significant challenge to the therapeutic efficacy of the AAV gene delivery system. More recently, members of the Bocaparvovirus genus of the Parvoviridae have also been developed as viral gene delivery vectors, also for treating monogenetic diseases. However, high level of seroprevalence of host antibodies against AAVs and bocaviruses (BoVs), at ≥70%, represents a major challenge to full therapeutic realization of both systems. The primary focus of this project has been to characterize the antigenic structures of primate AAVs, using mouse monoclonal antibodies (Mabs), as they relate to capsid determinants of receptor attachment, tissue tropism, and transduction efficiency (gene expression). We pioneered the use of this information for molecular engineering of AAV vectors able to escape antibody recognition and currently under evaluation as potential clinical vectors. However, there is need to confirm that the “polyclonal” information obtained by mapping several mouse Mabs for each AAV serotype studied recapitulates the polyclonal human response. In this renewal application, we will characterize the ability of human and non-human primate (NHP) sera to neutralize or bind and not neutralize vector transduction. This will guide the engineering of antibody escape and/or transduction efficacy and thus therapeutic utility. We expand our viral models to include non-primate AAVs and BoV vectors in an effort to expand the pool of parvoviral vectors available for use. Our three specific aims will ask four new questions: (1) “Do primate antibodies share epitopes with the previously described murine Mabs?” (2) “Do the binding sites of neutralizing and non- neutralizing binding antibodies overlap”? (3) “Do non-primate AAVs naturally escape pre-existing neutralizing primate antibodies and capable of transducing human cells?” And (4) “Can we engineer the antigenic sites on BoV vectors to evade neutralization by antibodies while retaining or improving the parental transduction efficiency?”. We will use cryo-electron microscopy and image reconstruction to determine high-resolution structures of non-primate AAVs and BoV capsids, alone and in complex with glycan receptors, to ≤3 Å resolution and the structures of AAV/BoV capsid – human/NHP antibodies to between 3 to 4 Å resolution. This is routine in our group. We will use the information obtained to engineer vectors that retain their cell binding properties but evade recognition by human/NHP. We will evaluate these vectors in vitro and in vivo in the presence of IgG and IVIG, respectively. We will create new clinical biologics, as was done in the past funding round, to expand the parvovirus viral vector repertoire, thus the number of treatable diseases targets, and cohort of treatable patients.

腺相关病毒（aav）是依赖病毒属的ssDNA包装病毒

项目成果

期刊论文数量（101）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（12）

Capsid Structure of Aleutian Mink Disease Virus and Human Parvovirus 4: New Faces in the Parvovirus Family Portrait.

DOI：
10.3390/v14102219
发表时间：
2022-10-09
期刊：
Viruses
影响因子：
0
作者：
Lakshmanan R;Mietzsch M;Jimenez Ybargollin A;Chipman P;Fu X;Qiu J;Söderlund-Venermo M;McKenna R
通讯作者：
McKenna R

Completion of the AAV Structural Atlas: Serotype Capsid Structures Reveals Clade-Specific Features.

DOI：
10.3390/v13010101
发表时间：
2021-01-13
期刊：
Viruses
影响因子：
0
作者：
Mietzsch M;Jose A;Chipman P;Bhattacharya N;Daneshparvar N;McKenna R;Agbandje-McKenna M
通讯作者：
Agbandje-McKenna M

Optimizing the Targeting of Mouse Parvovirus 1 to Murine Melanoma Selects for Recombinant Genomes and Novel Mutations in the Viral Capsid Gene.

DOI：
10.3390/v10020054
发表时间：
2018-01-30
期刊：
Viruses
影响因子：
0
作者：
Marr M;D'Abramo A;Pittman N;Agbandje-McKenna M;Cotmore SF;Tattersall P
通讯作者：
Tattersall P

Development of new adeno-associated virus capsid variants for targeted gene delivery to human cardiomyocytes.

DOI：
10.1016/j.omtm.2023.08.010
发表时间：
2023-09-14
期刊：
MOLECULAR THERAPY METHODS & CLINICAL DEVELOPMENT
影响因子：
0
作者：
Kok, Cindy Y.;Tsurusaki, Shinya;Cabanes-Creus, Marti;Igoor, Sindhu;Rao, Renuka;Skelton, Rhys;Liao, Sophia H. Y.;Ginn, Samantha L.;Knight, Maddison;Scott, Suzanne;Mietzsch, Mario;Fitzsimmons, Rebecca;Miller, Jessica;Mohamed, Tamer M. A.;McKenna, Robert;Chong, James J. H.;Hill, Adam P.;Hudson, James E.;Alexander, Ian E.;Lisowski, Leszek;Kizana, Eddy
通讯作者：
Kizana, Eddy

BAAV transcytosis requires an interaction with beta-1-4 linked- glucosamine and gp96.