A novel rat sperm cryopreservation and artificial insemination system

一种新型大鼠精子冷冻保存及人工授精系统

• 批准号: 10544845
• 负责人: Barbara Jean Stone
• 金额: $ 25.89万
• 依托单位: PARATECHS CORP.
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-15 至 2024-09-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10544845
• 关键词: Absence of pain sensation; Anesthesia procedures; Animal Experimentation; Animal Model; Animal Welfare; Animals; Archives; Artificial Insemination; Assisted Reproductive Technology; Behavior; Biomedical Research; Biotechnology; Breeding; Contracts; Core Facility; Cost Savings; Cryopreservation; Device or Instrument Development; Devices; Disease; Embryo Transfer; Female; Fertilization in Vitro; Freezing; Funding Opportunities; Generations; Genetic Drift; Goals; Health; Horns; Human; Insemination; Laboratory Animal Models; Laboratory Animal Production and Facilities; Laboratory Animals; Laboratory Procedures; Maintenance; Methods; Modeling; Mouse Strains; Mus; Natural Disasters; Operative Surgical Procedures; Physiology; Procedures; Process; Rat Strains; Rattus; Recovery; Research Personnel; Rodent; Sampling; Services; Sperm Motility; System; Techniques; Technology; Testing; Toxicology; United States National Institutes of Health; Uterus; animal care; assisted reproduction; assistive reproductive technique; cell motility; cohesion; cost; design; drug development; embryo culture; experience; experimental study; improved; innovation; meetings; novel; preservation; protocol development; response; sperm cell; sperm cryopreservation; success; tool; welfare

Summary ParaTechs Corporation aims to provide researchers with advanced technology and methods to simplify procedures for laboratory animal models. This proposal is a direct response to PAR-21-225; Novel Tools and Devices for Animal Research Facilities and to Support the Care of Animal Models. The goal is to improve vivarium management through cryopreservation, strategic breeding, and organizational approaches currently unavailable for rats. The rat artificial insemination system (RAIS) is a complete rat sperm cryorecovery system. This system will revolutionize cryopreservation practices and vivarium management for rat models. First, sperm is cryopreserved in a “Cryodropper” device, which will quickly and easily freeze rat sperm. Second, sperm is recovered in a novel motility enhancing device (MED) which selects for motile sperm, removes cryoprotectants, and concentrates the sperm sample. The third part of the system is a novel device, which is used to transfer concentrated motile sperm to the uterine horn of recipient female rats. This non-surgical insemination device (NSID) provides a non-surgical alternative to traditional surgical artificial insemination and requires no anesthesia or analgesia. Each innovative part of the system provides a critical function for rat cryorecovery and together provide a cohesive system for rapid, inexpensive, and efficient vivarium management. Feasibility of the RAIS will be proven in the following Aims; 1) optimize the Cryodropper for rat sperm cryopreservation, 2) manufacture and test the MED for motile sperm enrichment and recovery, and 3) manufacture and test the rat NSID device with fresh and cryopreserved sperm. The system will provide substantial cost savings versus contracted cryopreservation services and increase the simplicity and reliability of rat sperm cryopreservation procedures. RAIS will directly benefit the welfare of laboratory animals by reducing the number of animals kept in idle colonies, improving vivarium management options, and protecting rat models from catastrophic loss due to natural disasters, genetic drift, disease, and breeding problems. RAIS is designed to provide both a reduction in animal numbers and a refinement to minimize animal suffering and improve welfare; meeting the requirements of the 3Rs of animal research.

摘要 ParaTechs Corporation的目标是为研究人员提供先进的技术和方法，以简化 实验动物模型的程序。该提案是对PAR-21-225的直接响应；新工具和 用于动物研究设施和支持关爱动物模型的设备。我们的目标是改进 目前通过冷冻保存、战略育种和组织化方法管理卵泡的方法 不适用于老鼠。大鼠人工授精系统(RAIS)是一个完整的大鼠精子冷冻回收系统。 该系统将对大鼠模型的冷冻保存实践和管理产生革命性的影响。第一，精子 冷冻保存在“冷冻滴管”装置中，可以快速方便地冷冻大鼠精子。第二，精子是 在一种新型的运动增强装置(MED)中回收，该装置选择可活动的精子，去除冷冻保护剂， 然后浓缩精子样本。系统的第三部分是一种新型的设备，用于传输 将活动精子浓缩到受体雌性大鼠的子宫角。这种非手术人工授精装置 (NSID)提供了一种传统外科人工授精的非手术替代方案，不需要麻醉 或者是止痛药。该系统的每个创新部分都为大鼠冷冻复苏提供了关键功能，并共同 提供一个凝聚力强的系统，实现快速、廉价和高效的隔膜管理。区域创新系统的可行性 将在以下方面得到验证：1)优化用于大鼠精子冷冻的冷冻管，2)制造 并测试MED对活动精子的浓缩和回收，以及3)制造和测试大鼠NSID设备 用新鲜的和冷冻保存的精子。与合同相比，该系统将节省大量成本 提供冷冻服务，并提高大鼠精子冷冻程序的简单性和可靠性。 RAIS将通过减少闲置动物的数量直接造福于实验动物 克隆，改进管理选项，并保护大鼠模型免受因 自然灾害、基因漂移、疾病和繁殖问题。RAIS的设计目的是既减少 动物数量和精细化以最大限度地减少动物的痛苦并改善福利；满足要求 在动物研究的3R中。