A novel rat sperm cryopreservation and artificial insemination system

一种新型大鼠精子冷冻保存及人工授精系统

• 批准号: 10544845
• 负责人: Barbara Jean Stone
• 金额: $ 25.89万
• 依托单位: PARATECHS CORP.
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-15 至 2024-09-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10544845
• 关键词: Absence of pain sensation; Anesthesia procedures; Animal Experimentation; Animal Model; Animal Welfare; Animals; Archives; Artificial Insemination; Assisted Reproductive Technology; Behavior; Biomedical Research; Biotechnology; Breeding; Contracts; Core Facility; Cost Savings; Cryopreservation; Device or Instrument Development; Devices; Disease; Embryo Transfer; Female; Fertilization in Vitro; Freezing; Funding Opportunities; Generations; Genetic Drift; Goals; Health; Horns; Human; Insemination; Laboratory Animal Models; Laboratory Animal Production and Facilities; Laboratory Animals; Laboratory Procedures; Maintenance; Methods; Modeling; Mouse Strains; Mus; Natural Disasters; Operative Surgical Procedures; Physiology; Procedures; Process; Rat Strains; Rattus; Recovery; Research Personnel; Rodent; Sampling; Services; Sperm Motility; System; Techniques; Technology; Testing; Toxicology; United States National Institutes of Health; Uterus; animal care; assisted reproduction; assistive reproductive technique; cell motility; cohesion; cost; design; drug development; embryo culture; experience; experimental study; improved; innovation; meetings; novel; preservation; protocol development; response; sperm cell; sperm cryopreservation; success; tool; welfare

Summary ParaTechs Corporation aims to provide researchers with advanced technology and methods to simplify procedures for laboratory animal models. This proposal is a direct response to PAR-21-225; Novel Tools and Devices for Animal Research Facilities and to Support the Care of Animal Models. The goal is to improve vivarium management through cryopreservation, strategic breeding, and organizational approaches currently unavailable for rats. The rat artificial insemination system (RAIS) is a complete rat sperm cryorecovery system. This system will revolutionize cryopreservation practices and vivarium management for rat models. First, sperm is cryopreserved in a “Cryodropper” device, which will quickly and easily freeze rat sperm. Second, sperm is recovered in a novel motility enhancing device (MED) which selects for motile sperm, removes cryoprotectants, and concentrates the sperm sample. The third part of the system is a novel device, which is used to transfer concentrated motile sperm to the uterine horn of recipient female rats. This non-surgical insemination device (NSID) provides a non-surgical alternative to traditional surgical artificial insemination and requires no anesthesia or analgesia. Each innovative part of the system provides a critical function for rat cryorecovery and together provide a cohesive system for rapid, inexpensive, and efficient vivarium management. Feasibility of the RAIS will be proven in the following Aims; 1) optimize the Cryodropper for rat sperm cryopreservation, 2) manufacture and test the MED for motile sperm enrichment and recovery, and 3) manufacture and test the rat NSID device with fresh and cryopreserved sperm. The system will provide substantial cost savings versus contracted cryopreservation services and increase the simplicity and reliability of rat sperm cryopreservation procedures. RAIS will directly benefit the welfare of laboratory animals by reducing the number of animals kept in idle colonies, improving vivarium management options, and protecting rat models from catastrophic loss due to natural disasters, genetic drift, disease, and breeding problems. RAIS is designed to provide both a reduction in animal numbers and a refinement to minimize animal suffering and improve welfare; meeting the requirements of the 3Rs of animal research.

总结 ParaTechs公司旨在为研究人员提供先进的技术和方法， 实验室动物模型。本提案是对PAR-21-225《新型工具和 用于动物研究机构和支持动物模型护理的器械。目标是提高 目前，通过冷冻保存、战略育种和组织方法进行的动物园管理 不适合老鼠。大鼠人工授精系统（RAIS）是一套完整的大鼠精子冷冻复苏系统。 该系统将彻底改变大鼠模型的冷冻保存实践和动物饲养管理。第一，精子 被冷冻保存在一个“冷冻滴管”装置中，该装置可以快速轻松地冷冻大鼠精子。其次，精子是 在一种新型的运动增强装置（MED）中回收，该装置选择运动精子，去除冷冻保护剂， 浓缩精子样本系统的第三部分是一种新颖的装置，用于传输 将浓缩的活动精子注入受体雌性大鼠的子宫角。这种非手术授精装置 （NSID）提供了一种非手术替代传统的手术人工授精，不需要麻醉 或镇痛。系统的每个创新部分都为大鼠冷冻复苏提供了关键功能， 为快速、廉价和有效的动物饲养管理提供了一个有凝聚力的系统。RAIS的可行性 将在以下目标中得到证明：1）优化用于大鼠精子冷冻保存的冷冻滴管，2）制造 并测试MED对活动精子的富集和回收; 3）制作和测试大鼠NSID装置 新鲜冷冻的精子该系统将比订约承办事务节省大量费用。 冷冻保存服务，并增加大鼠精子冷冻保存程序的简单性和可靠性。 RAIS将通过减少闲置动物的数量直接有利于实验室动物的福利 殖民地，改善饲养管理的选择，并保护大鼠模型的灾难性损失， 自然灾害、遗传漂变、疾病和繁殖问题。RAIS旨在减少 动物数量和优化，以最大限度地减少动物痛苦并提高福利;符合要求 动物研究的3R原则