Impact of Bacteriophages on Virulence and Transmission of Vibrio cholerae

噬菌体对霍乱弧菌毒力和传播的影响

• 批准号: 10548231
• 负责人: Andrew Camilli
• 金额: $ 46.5万
• 依托单位: TUFTS UNIVERSITY BOSTON
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-05-15 至 2025-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10548231
• 关键词: Adsorption; Animals; Area; Bacteria; Bacteriophages; Biological Assay; Cells; Cholera; Cholera Toxin; Clustered Regularly Interspaced Short Palindromic Repeats; Cyclophosphamide; DNA; Data; Disease; Environment; Evolution; Fiber; Gastrointestinal tract structure; Genes; Genome; Household; In Vitro; Infant; Infection; Life Cycle Stages; Lytic; Measures; Modeling; Mus; Oral; Oryctolagus cuniculus; Pathogenesis; Phenotype; Plaque Assay; Play; Population; Population Sizes; Predatory Behavior; Production; Prophage Inductions; Prophages; Publishing; Role; Small Intestines; Symptoms; Tail; Testing; Time; Toxin; Vibrio cholerae; Virulence; Virulence Factors; Virulent; Virus; Water; biotypes; contaminated water; deep sequencing; density; diarrheal disease; disease transmission; experimental study; fitness; mutant; pathogen; pathogenic bacteria; prevent; prophylactic; transmission process; waterborne; waterborne pathogen

PROJECT SUMMARY Bacteriophages (phages) are viruses that specifically infect bacteria. Phages have been shown to play important roles in the life cycle of many bacterial pathogens, particularly water-borne pathogens that cause gastrointestinal tract infections. For example, the lysogenic phages, which integrate their DNA into the host genome, can play positive roles by providing virulence factors such as toxins that enhance the virulence of their host bacterium. Other phages known as lytic or virulent phages can play negative roles by infecting and killing their host bacterium, thus reducing the number of the pathogen below the threshold needed to cause disease. Vibrio cholerae is a water-borne pathogen that infects the small intestine to cause the severe diarrheal disease cholera. Although it is well known that a lysogenic phage called the CTX phage enhances virulence by providing the genes encoding cholera toxin, our recently published and preliminary data indicate a pervasive negative role for several virulent phages that are commonly found in cholera endemic areas around the Bay of Bengal. These virulent phages appear to reduce the virulence, transmission, and potentially dissemination of V. cholerae. However, there is much we still do not know about these phages in terms of their ability to kill V. cholerae in the environment, to inhibit fecal-oral transmission of cholera such as occurs frequently within households, and their impact on the emergence of new V. cholerae strains. In this project, we will investigate each of these areas. In addition, we will study the importance of another lysogenic phage called Kappa in the virulence of V. cholerae.

项目总结