Viral Testing Core

病毒检测核心

• 批准号: 10548595
• 负责人: Smriti Mehra
• 金额: $ 3.08万
• 依托单位: TEXAS BIOMEDICAL RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-30 至 2027-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10548595
• 关键词: 2019-nCoV; AIDS vaccine development; Acquired Immunodeficiency Syndrome; Affect; Algorithms; Animal Model; Animals; Antibodies; Antibody titer measurement; Antiviral Therapy; Biological Assay; Breeding; COVID-19 pandemic; COVID-19 testing; California; Caribbean region; Chagas Disease; Consensus; Data; Decision Making; Development; Ensure; Equipment; Event; Exposure to; Goals; Grant; HIV; Health; Herpesvirus 1; Human; Human Resources; Immune response; Immunization; Immunization Programs; Immunoassay; Immunology; Infection; Investigation; Laboratories; Macaca; Macaca mulatta; Maintenance; Measles; Measles virus; Methods; Monitor; Monkeys; Mucous Membrane; Oregon; Parasites; Pathogen detection; Patients; Pest Control; Prevalence; Primates; Principal Investigator; Procedures; Reagent; Recombinants; Recommendation; Reliability of Results; Research; Research Personnel; Research Support; Reverse Transcriptase Polymerase Chain Reaction; Rhesus; Risk; SARS-CoV-2 antibody; SIV; Sampling; Serology; Serology test; Serum; Simian Retroviruses; System; Techniques; Testing; Time; Trypanosoma cruzi; U-Series Cooperative Agreements; United States National Institutes of Health; Viral; Viral Pathogenesis; Virus; Washington; animal care; antibody detection; effectiveness evaluation; efficacy evaluation; germ free condition; improved; instrument; interest; member; pathogen; programs; screening; simian human immunodeficiency virus; viral RNA; working group

Abstract – Core B: Viral Testing Core The Viral Testing Core is a critical component for the maintenance of a Specific Pathogen Free (SPF) Indian rhesus macaque (Ind RM) (Macaca mulatta) breeding colony. Rhesus macaques infected with the Simian Immunodeficiency Virus (SIV) are the preferred animal model for AIDS studies and are used to examine many aspects of viral pathogenesis, AIDS vaccine development, and exploratory new systemic and mucosal antiviral therapies, including early events not readily studied in human patients. The presence of certain viruses can confound the results of AIDS-related investigations. SPF colonies have been developed using state-of-the art assays to eliminate animals with simian immunodeficiency virus (SIV), Type D simian retrovirus (SRV) simian T- lymphotropic virus (STLV-1); and herpes B virus (Macacine herpesvirus-1), which is a potential health risk for personnel handling the animals. The goal of the Viral Testing Core is to provide techniques and expertise to assure the continuing SPF status of the Ind RM breeding colony at the Southwest National Primate Research Center (SNPRC). In order to fulfill this goal, the following Specific Aims will be completed: Specific Aim 1: to provide a reliable and sensitive in-house viral screening method. This screening method is intended to produce reliable results in a fast turnaround time. We will continue to use the Charles River Laboratories (CRL) Multiplex Fluorescent ImmunoAssay (FMIA) for serological screening of all animals, which represents one of the current state-of-the-art surveillance techniques. Specific Aim 2: to increase the research value of the SNPRC Ind RM breeding colony by expanding serology testing. The core is ever evolving in the use of state-of-the-art techniques and expanding to detect other pathogens of interest. In recent years, we extended our serology testing to include detection of antibodies against measles virus and Trypanosoma cruzi. Presence of antibodies to measles is used to confirm immune responses to the colony active measles immunization effort. Serological screening for antibodies to the causative agent of Chagas Disease, along with PCR confirmatory testing, is used to 1) assist animal selection; 2) to determine the prevalence of this infection in the NHP colonies; and 3) to improve and evaluate the effectiveness of our pest control system. Finally, SNPRC has recently screened the SPF colony for SARS-CoV-2 by both serology and PCR. Following recommendations developed by the National Primate Research Center Pathogen Detection Working Group, the Viral Testing Core has developed an algorithm that incorporates results from the serological viral screening with both serological and PCR confirmatory assays. Additionally, the Core will continue to participate in activities proposed by the national Breeding Colony Management Consortium (BCMC), which frequently distributes serum panels for proficiency testing among its laboratory members with the intent to monitor and ensure acceptable quality testing algorithms, reagents, methods, equipment, and personnel.

摘要-核心B：病毒检测核心 病毒检测核心是维持无特定病原体（SPF）印度人的关键组成部分 恒河猴（IndRM）（Macaca mulatta）繁殖群。感染猿猴的恒河猴 免疫缺陷病毒（SIV）是艾滋病研究的首选动物模型，并用于检查许多 病毒的发病机制，艾滋病疫苗的开发，以及探索新的全身和粘膜抗病毒方面 治疗，包括在人类患者中不容易研究的早期事件。某些病毒的存在可以 混淆了艾滋病相关调查的结果。SPF菌落已经使用最先进的 测定以消除具有猿猴免疫缺陷病毒（SIV）、D型猿猴逆转录病毒（SRV）、猿猴T- 嗜淋巴细胞病毒（STLV-1）和疱疹B病毒（猕猴疱疹病毒-1），这是一个潜在的健康风险， 管理动物的人员。病毒检测核心的目标是提供技术和专业知识， 确保西南国家灵长类动物研究所的IndRM繁殖群的持续SPF状态 中心（SNPRC）。为了实现这一目标，将完成以下具体目标：具体目标1： 提供可靠和灵敏的内部病毒筛查方法。这种筛选方法旨在产生 在快速的周转时间内获得可靠的结果。我们将继续使用Charles River Laboratories（CRL）Multiplex 荧光免疫分析（FMIA）用于所有动物的血清学筛查，这代表了当前 最先进的监视技术具体目标2：提高SNPRC Ind RM的研究价值 通过扩大血清学试验繁殖群体。核心是不断发展的使用国家的最先进的技术 并扩展到检测其他感兴趣的病原体。近年来，我们扩大了血清学检测范围， 麻疹病毒和克氏锥虫抗体检测。使用麻疹抗体的存在 以确认对殖民地群体的免疫应答积极的麻疹免疫努力。血清学筛查 恰加斯病病原体的抗体，沿着PCR确证试验，用于1）协助 动物选择; 2）确定NHP群体中这种感染的流行率;以及3）改善和 评估我们的虫害控制系统的有效性。最后，SNPRC最近对SPF群体进行了筛选， SARS-CoV-2的血清学和PCR检测。根据国家灵长类动物委员会提出的建议， 研究中心病原体检测工作组，病毒检测核心已经开发出一种算法， 将血清学病毒筛查结果与血清学和PCR确证性试验结合起来。 此外，核心将继续参与国家育种群提出的活动 管理联盟（BCMC），该联盟经常在其成员之间分发用于能力验证的血清样本组。 实验室成员，旨在监控和确保可接受的质量测试算法，试剂， 方法、设备和人员。