Viral Testing Core

摘要-核心B：病毒检测核心 病毒检测核心是维护无特定病原体(SPF)印度人的关键组件 恒河猴(Ind Rm)(Macaca Mulatta)繁殖群体。感染猿猴的恒河猴 免疫缺陷病毒(SIV)是艾滋病研究的首选动物模型，并被用于检测许多 病毒致病机制、艾滋病疫苗开发和探索新的全身和粘膜抗病毒药物 治疗，包括在人类患者中不容易研究的早期事件。某些病毒的存在可能 混淆了与艾滋病有关的调查结果。SPF菌落是用最先进的技术开发出来的 消除猴免疫缺陷病毒(SIV)、D型猴逆转录病毒(SRV)猴T- 嗜淋巴病毒(STLV-1)；和B型疱疹病毒(猕猴疱疹病毒-1)，这是潜在的健康风险 负责处理动物的人员。病毒检测核心的目标是提供技术和专业知识 确保西南国家灵长类研究会印度繁育群体持续SPF地位 中心(SNPRC)。为实现这一目标，将完成以下具体目标：具体目标1： 提供可靠和灵敏的内部病毒筛查方法。这种筛选方法旨在生产 结果可靠，周转时间短。我们将继续使用Charles River实验室(CRL)多厅 用于对所有动物进行血清学筛查的荧光免疫分析(FMIA)，它代表了当前 最先进的监控技术。具体目标2：增加SNPRC IND RM的研究价值 通过扩大血清学检测来培育菌落。在使用最先进的技术方面，核心不断发展 并扩展到检测其他感兴趣的病原体。近年来，我们将我们的血清学检测扩展到包括 麻疹病毒和克氏锥虫抗体的检测。使用麻疹抗体的存在 确认对群体主动麻疹免疫工作的免疫反应。猪瘟的血清学筛查 恰加斯病的病原体抗体与聚合酶链式反应确证试验一起被用来辅助1 动物选择；2)确定这种感染在NHP群体中的流行率；以及3)改善和 评估我们的虫害控制系统的有效性。最后，SNPRC最近对SPF菌落进行了筛查 用血清学和聚合酶链式反应检测SARS-CoV-2。根据国家灵长类动物制定的建议 研究中心病原体检测工作组，病毒检测核心开发了一种算法， 将血清学病毒筛查的结果与血清学和聚合酶链式反应确证试验相结合。 此外，核心将继续参与国家繁育群体提出的活动 管理联盟(BCMC)，该联盟经常在其 实验室成员，意在监督和确保可接受的质量测试算法、试剂、 方法、设备和人员。

Abstract – Core B: Viral Testing Core The Viral Testing Core is a critical component for the maintenance of a Specific Pathogen Free (SPF) Indian rhesus macaque (Ind RM) (Macaca mulatta) breeding colony. Rhesus macaques infected with the Simian Immunodeficiency Virus (SIV) are the preferred animal model for AIDS studies and are used to examine many aspects of viral pathogenesis, AIDS vaccine development, and exploratory new systemic and mucosal antiviral therapies, including early events not readily studied in human patients. The presence of certain viruses can confound the results of AIDS-related investigations. SPF colonies have been developed using state-of-the art assays to eliminate animals with simian immunodeficiency virus (SIV), Type D simian retrovirus (SRV) simian T- lymphotropic virus (STLV-1); and herpes B virus (Macacine herpesvirus-1), which is a potential health risk for personnel handling the animals. The goal of the Viral Testing Core is to provide techniques and expertise to assure the continuing SPF status of the Ind RM breeding colony at the Southwest National Primate Research Center (SNPRC). In order to fulfill this goal, the following Specific Aims will be completed: Specific Aim 1: to provide a reliable and sensitive in-house viral screening method. This screening method is intended to produce reliable results in a fast turnaround time. We will continue to use the Charles River Laboratories (CRL) Multiplex Fluorescent ImmunoAssay (FMIA) for serological screening of all animals, which represents one of the current state-of-the-art surveillance techniques. Specific Aim 2: to increase the research value of the SNPRC Ind RM breeding colony by expanding serology testing. The core is ever evolving in the use of state-of-the-art techniques and expanding to detect other pathogens of interest. In recent years, we extended our serology testing to include detection of antibodies against measles virus and Trypanosoma cruzi. Presence of antibodies to measles is used to confirm immune responses to the colony active measles immunization effort. Serological screening for antibodies to the causative agent of Chagas Disease, along with PCR confirmatory testing, is used to 1) assist animal selection; 2) to determine the prevalence of this infection in the NHP colonies; and 3) to improve and evaluate the effectiveness of our pest control system. Finally, SNPRC has recently screened the SPF colony for SARS-CoV-2 by both serology and PCR. Following recommendations developed by the National Primate Research Center Pathogen Detection Working Group, the Viral Testing Core has developed an algorithm that incorporates results from the serological viral screening with both serological and PCR confirmatory assays. Additionally, the Core will continue to participate in activities proposed by the national Breeding Colony Management Consortium (BCMC), which frequently distributes serum panels for proficiency testing among its laboratory members with the intent to monitor and ensure acceptable quality testing algorithms, reagents, methods, equipment, and personnel.