JHU-Mayo-NIA Murine Senescence Mapping Program (JMN-MSMP)

JHU-Mayo-NIA 小鼠衰老图谱计划 (JMN-MSMP) 的总体目标是创建一个图谱 整个生命周期中小鼠组织的衰老。该提案包含独特和新颖的生物 以及绘制小鼠组织中衰老细胞（SnC）图谱的计算资源和策略。生物的 资源包括多种品系的老化小鼠群体，包括那些带有报告基因的群体 SnC。我们还可以使用强大的鼠组织库和老化组织编目基础设施， 包括那些患有与年龄相关的病症的人。计算资源包括单细胞测序和空间 转录组学专业知识、可识别 SnC 并阐明细胞通讯的迁移学习算法 从单细胞数据集以及基因组学和多种成像方式的整合来识别组织中的 SnC 在 2D 和 3D 中。对于该提案，JMN-MSMP 团队将首先关注制定出现的时间表 美国国家老龄化研究所小鼠衰老项目组织中血浆/血清中衰老标志物的研究 包括大脑。重要的是，我们已经存档了两种基因不同的实验室小鼠品系的组织 两性的整个生命周期，这将使我们能够尽快实施严格的多维分析 由于该计划已获得资助。随后将对选定组织进行深入的单细胞衰老分析 映射。 JMN-MSMP 将由三个核心部分组成：行政核心 (AC)、生物核心 分析核心 (BAC) 和数据分析核心 (DAC)。 JMN-MSMP 的具体目标是： 目标 1. JMN-MSMP 总体研究策略：整合基因组学和成像策略，构建 小鼠组织衰老综合图谱，并定义衰老的生理影响 细胞。 目标 2. JMN-MSMP 总体管理战略、目标和里程碑：提供基础设施以支持 为创建地图集而进行的综合研究工作。 目标 3. JMN-MSMP 资源共享：确保组织、数据、方法和技术可用于 其他 TMC，并制定适当的数据存储和共享政策。

The overall goal of the JHU-Mayo-NIA Murine Senescence Mapping Program (JMN-MSMP) is to create an Atlas of Senescence in Murine Tissues across the lifespan. The proposal encompasses unique and novel biological and computational resources and strategies to map senescent cells (SnCs) in murine tissues. Biological resources include aging mouse colonies of multiple strains, including those with reporters for identification of SnCs. We also have access to a robust murine tissue bank and cataloging infrastructure for aging tissues, including those with age-related pathology. Computational resources include single-cell sequencing and spatial transcriptomics expertise, transfer learning algorithms that can identify SnCs and elucidate cell communication from single-cell datasets, and integration of genomics and multiple imaging modalities to identify SnCs in tissues in 2D and 3D. For this proposal, the JMN-MSMP team will focus first on developing a timeline of the emergence of senescence markers in plasma/serum in tissues from the National Institute of Aging murine aging program including brain. Importantly, we have archived tissues from two genetically distinct strains of laboratory mice of both sexes over their lifespan that will allow us to implement a rigorous and multidimensional analysis as soon as the Program is funded. This will be followed by in depth single cell analysis of selected tissues for senescence mapping. The JMN-MSMP will consist of three Core components: the Administrative Core (AC), Biological Analysis Core (BAC), and Data Analysis Core (DAC). The Specific Aims of the JMN-MSMP are: Aim 1. Overall JMN-MSMP Research Strategy: Integrate genomics and imaging strategies to build a comprehensive Atlas of Senescence in Murine Tissues, and to define the physiological impact of senescent cells. Aim 2. Overall JMN-MSMP Management Strategy, Goals, and Milestones: Provide infrastructure to support an integrated research effort toward creation of the Atlas. Aim 3. JMN-MSMP Resource Sharing: Ensure that tissues, data, methods, and technologies are available to other TMCs, and that appropriate data deposition and sharing policies are in place.