JHU-Mayo-NIA Murine Senescence Mapping Program (JMN-MSMP)

JHU-Mayo-NIA小鼠衰老图谱计划(JMN-MSMP)的总体目标是创建一份地图集 小鼠组织在整个生命周期中的衰老。该提案包含了独特和新颖的生物学 以及绘制小鼠组织中衰老细胞(SNC)图谱的计算资源和策略。生物学 资源包括多个品系的老化小鼠群体，包括那些为鉴定 SNCS。我们还可以使用强大的小鼠组织库和对老化组织进行编目的基础设施， 包括那些患有年龄相关病理的人。计算资源包括单细胞测序和空间 转录学专业知识，转移学习算法，可以识别SNC并阐明细胞通信 从单细胞数据集，以及基因组学和多种成像模式的集成来识别组织中的SNCs 在2D和3D中。对于这项提议，JMN-MSMP团队将首先专注于制定出现的时间表 美国国立老龄研究所小鼠衰老项目中血浆/血清组织中衰老标志物的研究 包括大脑。重要的是，我们保存了两种不同基因类型的实验室小鼠的组织 这将使我们能够尽快实施严格的多维分析 因为该计划得到了资金支持。随后将对选定的衰老组织进行深入的单细胞分析 映射。JMN-MSMP将由三个核心组件组成：管理核心(AC)、生物 分析核心(BAC)和数据分析核心(DAC)。JMN-MSMP的具体目标是： 目标1.整体JMN-MSMP研究战略：整合基因组学和成像战略，以建立 小鼠组织衰老综合图谱，以及衰老的生理影响 细胞。 目标2.JMN-MSMP总体管理战略、目标和里程碑：提供基础设施以支持 为创建地图集而进行的综合研究工作。 目标3.JMN-MSMP资源共享：确保组织、数据、方法和技术可用于 其他TMC，并且制定了适当的数据存储和共享政策。

The overall goal of the JHU-Mayo-NIA Murine Senescence Mapping Program (JMN-MSMP) is to create an Atlas of Senescence in Murine Tissues across the lifespan. The proposal encompasses unique and novel biological and computational resources and strategies to map senescent cells (SnCs) in murine tissues. Biological resources include aging mouse colonies of multiple strains, including those with reporters for identification of SnCs. We also have access to a robust murine tissue bank and cataloging infrastructure for aging tissues, including those with age-related pathology. Computational resources include single-cell sequencing and spatial transcriptomics expertise, transfer learning algorithms that can identify SnCs and elucidate cell communication from single-cell datasets, and integration of genomics and multiple imaging modalities to identify SnCs in tissues in 2D and 3D. For this proposal, the JMN-MSMP team will focus first on developing a timeline of the emergence of senescence markers in plasma/serum in tissues from the National Institute of Aging murine aging program including brain. Importantly, we have archived tissues from two genetically distinct strains of laboratory mice of both sexes over their lifespan that will allow us to implement a rigorous and multidimensional analysis as soon as the Program is funded. This will be followed by in depth single cell analysis of selected tissues for senescence mapping. The JMN-MSMP will consist of three Core components: the Administrative Core (AC), Biological Analysis Core (BAC), and Data Analysis Core (DAC). The Specific Aims of the JMN-MSMP are: Aim 1. Overall JMN-MSMP Research Strategy: Integrate genomics and imaging strategies to build a comprehensive Atlas of Senescence in Murine Tissues, and to define the physiological impact of senescent cells. Aim 2. Overall JMN-MSMP Management Strategy, Goals, and Milestones: Provide infrastructure to support an integrated research effort toward creation of the Atlas. Aim 3. JMN-MSMP Resource Sharing: Ensure that tissues, data, methods, and technologies are available to other TMCs, and that appropriate data deposition and sharing policies are in place.