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JHU-Mayo-NIA Murine Senescence Mapping Program (JMN-MSMP)

JHU-Mayo-NIA 小鼠衰老图谱计划 (JMN-MSMP)

基本信息

批准号：
10673112
负责人：
Darren Baker
金额：
$ 131.64万
依托单位：
JOHNS HOPKINS UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-08-01 至 2026-07-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10673112
关键词：
3-Dimensional Adipose tissue Age Aging Atlases Biological Biological Markers Bone Marrow Brain Cataloging Cell Aging Cell Communication Cells Code Collaborations Communication Computer Analysis Data Data Analyses Data Coordinating Center Data Science Data Set Decision Making Deposition Development Ensure Epigenetic Process FAIR principles Funding Genomics Goals Heterogeneity Hippocampus Human Image Immunology Infrastructure Kidney Laboratory mice Lead Leadership Liver Longevity Lymphoid Tissue Maps Methodology Methods Molecular Monitor Mouse Strains Mus National Institute on Aging Outcome Pathology Phenotype Physiological Physiology Plasma Policies Prevalence Process Productivity Proteomics Protocols documentation Reporter Reproducibility Research Research Personnel Resource Sharing Resources Sampling Serum Shipping Skeletal Muscle Skin Specimen Standardization Techniques Technology Tissue Banks Tissues age related animal data circulating biomarkers computing resources data integration data sharing data submission dimensional analysis imaging modality immunohistochemical markers in vivo innovation learning algorithm novel novel marker programs repository scale up screening senescence sex single cell analysis single cell sequencing single cell technology single-cell RNA sequencing timeline tissue archive tissue mapping transcriptomics transfer learning

项目摘要

The overall goal of the JHU-Mayo-NIA Murine Senescence Mapping Program (JMN-MSMP) is to create an Atlas of Senescence in Murine Tissues across the lifespan. The proposal encompasses unique and novel biological and computational resources and strategies to map senescent cells (SnCs) in murine tissues. Biological resources include aging mouse colonies of multiple strains, including those with reporters for identification of SnCs. We also have access to a robust murine tissue bank and cataloging infrastructure for aging tissues, including those with age-related pathology. Computational resources include single-cell sequencing and spatial transcriptomics expertise, transfer learning algorithms that can identify SnCs and elucidate cell communication from single-cell datasets, and integration of genomics and multiple imaging modalities to identify SnCs in tissues in 2D and 3D. For this proposal, the JMN-MSMP team will focus first on developing a timeline of the emergence of senescence markers in plasma/serum in tissues from the National Institute of Aging murine aging program including brain. Importantly, we have archived tissues from two genetically distinct strains of laboratory mice of both sexes over their lifespan that will allow us to implement a rigorous and multidimensional analysis as soon as the Program is funded. This will be followed by in depth single cell analysis of selected tissues for senescence mapping. The JMN-MSMP will consist of three Core components: the Administrative Core (AC), Biological Analysis Core (BAC), and Data Analysis Core (DAC). The Specific Aims of the JMN-MSMP are: Aim 1. Overall JMN-MSMP Research Strategy: Integrate genomics and imaging strategies to build a comprehensive Atlas of Senescence in Murine Tissues, and to define the physiological impact of senescent cells. Aim 2. Overall JMN-MSMP Management Strategy, Goals, and Milestones: Provide infrastructure to support an integrated research effort toward creation of the Atlas. Aim 3. JMN-MSMP Resource Sharing: Ensure that tissues, data, methods, and technologies are available to other TMCs, and that appropriate data deposition and sharing policies are in place.

JHU-Mayo-NIA小鼠衰老绘图计划（JMN-MSMP）的总体目标是创建一个图谱小鼠组织中的衰老。该提案包括独特的和新颖的生物和计算资源和策略来映射小鼠组织中的衰老细胞（SnC）。生物资源包括多个品系的老化小鼠群体，包括那些具有用于鉴定 SnCs。我们还可以访问一个强大的小鼠组织库和老化组织的编目基础设施，包括那些与年龄有关的病理。计算资源包括单细胞测序和空间转录组学专业知识，转移学习算法，可以识别SnC和阐明细胞通讯从单细胞数据集，以及基因组学和多种成像方式的整合，以识别组织中的SnCs 2D和3D。对于这一建议，JMN-MSMP团队将首先集中精力制定一个出现的时间轴来自国家衰老研究所小鼠衰老计划的组织中血浆/血清中的衰老标志物包括大脑。重要的是，我们已经从两种遗传上不同的实验室小鼠品系中存档了组织，这将使我们能够尽快实施一项严格的多层面分析，因为该计划得到了资助。随后将对所选组织的衰老进行深入的单细胞分析映射. JMN-MSMP将由三个核心组成部分组成：行政核心（AC），生物分析核心（BAC）和数据分析核心（DAC）。JMN-MSMP的具体目标是：目标1.总体JMN-MSMP研究策略：整合基因组学和成像策略，小鼠组织衰老的综合图谱，并定义衰老的生理影响细胞目标2.总体JMN-MSMP管理战略、目标和里程碑：提供基础设施以支持综合研究工作，以创建地图集。目标3. JMN-MSMP资源共享：确保组织、数据、方法和技术可用于其他旅行管理公司，以及适当的数据存放和共享政策到位。