Optimising the Delivery of Superior Genetics through Advanced Genomic Selection of Bovine Embryos

通过牛胚胎的高级基因组选择优化优质遗传的传递

• 批准号: 101634
• 金额: $ 56.66万
• 依托单位: PARAGONVET LIMITED
• 依托单位国家: 英国
• 项目类别: Collaborative R&D
• 财政年份: 2013
• 资助国家: 英国
• 起止时间: 2013 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=101634
• 关键词: Optimising; Delivery; Superior; Genetics; through

This project will apply advanced breeding technologies to produce (both in-vivo and in-vitro) pre-implantation bovine embryos from which biopsies will be taken to interrogate their genomic makeup using single nucleotide polymorphism arrays (SNP chips). It is currently possible to screen dairy and beef cattle genomically, but responses to selection are impeded by waiting for the gestation of the calf on which genomic selection is performed. The screening of bovine embryos will optimise the delivery and amplification of superior genetics by advancing the time of selection and reducing “wastage” of unwanted calves. This project will therefore combine advanced embryo breeding technologies with state-of-the-art genomic screening (so called pre-implantation genetic diagnosis), and karyomapping (combining parental DNA information with the offspring's genomic information to provide more genetic detail). The project will develop strategies for optimal bovine embryo biopsy, cryopreservation and genomic screening of small cell numbers. It will provide proof of principle that the new technologies can be used to deliver superior genetics more efficiently to the breeding herd.

该项目将应用先进的育种技术来生产(体内和体外)植入前的牛胚胎，从这些胚胎中进行活组织检查，使用单核苷酸多态芯片(SNP芯片)来询问它们的基因组组成。目前，对奶牛和肉牛进行基因组筛选是可能的，但由于等待对其进行基因组选择的小牛怀孕，对选择的反应受到阻碍。牛胚胎的筛选将通过提前选择时间和减少不想要的小牛的“浪费”来优化优良遗传学的传递和放大。因此，该项目将把先进的胚胎育种技术与最先进的基因组筛选(所谓的植入前遗传诊断)和核映射(将父母的DNA信息与后代的基因组信息相结合，以提供更多遗传细节)结合起来。该项目将制定最佳牛胚胎活检、冷冻保存和小细胞数量基因组筛选的策略。它将提供原则上的证据，证明新技术可以被用来更有效地向育种牛群提供优越的遗传学。