Differential routing of valence information through the basolateral amygdala

通过基底外侧杏仁核的价信息的差异路由

• 批准号: 10552649
• 负责人: Kenneth Amaya
• 金额: $ 7.45万
• 依托单位: TUFTS UNIVERSITY BOSTON
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-02-01 至 2026-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10552649
• 关键词: Address; Amygdaloid structure; Animal Experimentation; Animals; Area; Basic Science; Behavior; Behavioral; Binge Eating; Brain; Cell Nucleus; Characteristics; Chronic; Code; Communication; Complex; Compulsive Behavior; Consultations; Corpus striatum structure; Data; Decision Making; Desire for food; Development; Disease; Electrophysiology (science); Exhibits; Fellowship; Food; Future; Gilles de la Tourette syndrome; Goals; Habits; Human; Individual; Investigation; Knowledge; Learning; Location; Mammals; Mental Health; Methodology; Methods; Mission; Motivation; Neurons; Obsessive-Compulsive Disorder; Outcome; Pattern; Performance; Personal Satisfaction; Phase; Phenotype; Physiological; Postdoctoral Fellow; Prefrontal Cortex; Problem Solving; Process; Rattus; Research; Research Personnel; Rewards; Rodent; Route; Running; Series; Signal Transduction; Stimulus; Structure; Substantia nigra structure; Techniques; Testing; Training; Translating; United States National Institutes of Health; Work; base; behavioral phenotyping; behavioral response; career; cell type; design; exercise regimen; expectation; experience; experimental study; flexibility; in vivo; in vivo calcium imaging; insight; interest; neural; neural correlate; neuromechanism; neuronal patterning; novel; pars compacta; psychologic; response

Project Summary Decision-making is critical for survival but is a complex process to understand at the level of brain function. Animals and humans alike can use two strategies to solve problems of decision-making: goal-directed or habitual behavior. Habits emerge late in training as animals transition from reliance on a goal-directed strategy to a habit with experience. Biologically, habits are known to develop via communication between a network of areas that span across the brain. Of particular interest for habit formation are the prefrontal cortex (IL), the sensorimotor striatum (DLS), dopaminergic projections to the striatum (SNc), and the central nucleus of the amygdala (CeA). Previous work has shown that a characteristic neuronal signature develops in IL and DLS, where as a habit develops, single-unit neuronal activity accentuates the beginning and end of actions of the behavior as it becomes well-learned. Importantly, as habits are comprised of multiple components (e.g. efficiency of action sequences and insensitivity to goal value), these individual features correlate with neural activity in distinct brain areas across the habit network (IL: outcome devaluation insensitivity, DLS: action vigor). However, there is a gap in knowledge in how activity in the central nucleus of the amygdala (CeA) codes habits or how that activity relates to the function of these other interconnected areas The objective of this project is to define how neural activity in CeA contributes to habit formation and whether a distinct component of behavior correlates with neural activity. The rationale is that the habit network is conserved across mammals as habits are ubiquitous and the potential for basic research to translate into bettering the human condition is high. The central hypothesis is that CeA neurons will develop a “chunking” pattern of activity that is necessary for a habit to develop, with behavioral correlates of this neural activity similar to those that correlate with IL. Guided by methods and data from Specific Aim 1, where behavioral phenotypes of habit and methodology to test habit formation are addressed, Specific Aim 2 will, through in-vivo electrophysiology, determine if CeA activity brackets in a manner similar to IL, DLS, and SNc as a habit forms on an elevated plus-maze task. Specific Aim 3 details a plan to identify and pursue a postdoctoral research fellowship upon completion of the dissertation work described in Specific Aim 2. Overall, the proposed experiment has the potential to provide mental health clinicians and researchers significant insight into how compulsive actions may arise from improperly timed amygdalar activity and will further define how neural activity can contribute to psychological phenomena such as habit formation.

项目摘要 决策对生存至关重要，但在大脑功能层面上理解是一个复杂的过程。 动物和人类一样可以使用两种策略来解决决策问题：目标导向策略或习惯性策略 行为。习惯在训练中出现较晚，因为动物从依赖目标导向的策略过渡到习惯 有经验的。从生物学上讲，习性是通过在下列区域之间的交流而形成的 横跨整个大脑。对习惯形成特别感兴趣的是前额叶皮质(IL)，这是一种感觉运动 纹状体(DLS)、向纹状体的多巴胺能投射(SNC)和杏仁中央核(CEA)。 先前的研究表明，IL和DLS会形成一种特有的神经元特征，这是一种习惯 发展，单一单位神经元活动强调行为的开始和结束，因为它 变得学识渊博。重要的是，AS习惯由多个组成部分组成(例如，行动效率 序列和对目标值不敏感)，这些个体特征与不同大脑中的神经活动相关 习惯网络的各个领域(IL：结果贬值不敏感，DLS：行动活力)。然而，有一个 杏仁中央核(CEA)的活动如何编码习惯或该活动如何编码的知识缺口 涉及到这些其他相互关联的区域的功能 这个项目的目标是确定CEA中的神经活动如何有助于习惯的形成和 行为的不同组成部分是否与神经活动相关。其基本原理是习惯网络 在哺乳动物中是保守的，因为习性无处不在，基础研究有可能转化为 改善人类状况是当务之急。中心假说是CEA神经元将形成“块状”。 一种养成习惯所必需的活动模式，与这种神经活动的行为关联类似 那些与IL相关的基因。由来自特定目标1的方法和数据指导，其中行为表型 讨论了习惯和测试习惯形成的方法，具体目标2将通过体内 电生理学，确定CEA活性括号是否以类似于IL、DLS和SNC的方式形成习惯 完成一个高架的加迷宫任务。具体目标3详细说明了确定和从事博士后研究的计划 在完成具体目标2中所述的论文工作后的研究金。总体而言，建议的 实验有可能为心理健康临床医生和研究人员提供重要的洞察 强迫行为可能源于杏仁核活动的不当时机，并将进一步定义神经活动 可以促成习惯养成等心理现象。