Shared Resource 11: Microscopy (MSR)

项目摘要-显微镜共享资源(MSR) MSR提供及时和高质量的服务，支持OSUCCC调查人员访问仪器， 就各种复杂的显微镜方法提供技术建议和培训支持。MSR 仪器在集中组织的资源中提供，所有设备都可供OSUCCC使用 经过培训的会员资格。在最后一次审查中，MSR是诊断共享资源组的一部分， 它被评为杰出的，关于领导力和使用的次要评论已经得到解决。 在当前的赠款周期内，在获取电子显微镜(低温、 透射和扫描电子显微镜)和共聚焦活细胞成像，包括增强的超 分辨率(SIM、STORM、TIRF)光学显微镜能力由NIH S10设备赠款资助。这个 MSR的具体目标是：1)为OSUCCC研究社区提供一种获得出版物的手段 用于手稿和拨款申请的高质量、更高分辨率的癌症研究图像；2) 在培训后对调查人员进行独立使用仪器的培训；3)提供教育 以及向OSUCCC研究人员提供咨询机会，以改进样品制备、成像技术 和图像分析；以及，作为向OSUCCC成员提供低温电子显微镜的发展目标 (冷冻-EM)。为实现这一发展目标，OSCUCC出资购买了两项文书，其中包括 纯化粒子和细胞粒子的纳米分辨率。在五年的赠款期间，MSR支持 319名调查员(32%为OSUCCC成员)，提供了5730小时的服务和44605小时的服务 仪器使用时间(OSUCCC成员占33.3%)。MSR发表了192篇论文(32篇；10篇 影响因素)和8个NCI补助金(2个K22、1个P01、1个P50、8个R01和1个U01)。MSR将支持显微镜 满足东苏丹特派团所有战略优先事项的需要。鉴于OSUCCC招聘情况强劲，对服务和新服务的需求 技术将会增加，产能将会扩大。为了与免疫肿瘤学需求保持一致，MSR将 成为免疫监测和发现平台(IMDP)的正式成员，以及双光子显微镜 将会更新。另外，MSR正在开发用于CET研究的低温FIB能力，它将开发 相关光电子显微镜和实时单分子细胞成像平台的协作 有了GEdSR。最后，MSR和GSR获得了一项新的拨款，以提供 支持细胞浓缩和单细胞测序。MSR得到了杰出机构的支持 利用与OSUCCC、OSU学院、OSU办公室的广泛伙伴关系获得的资源 来自俄亥俄州的研究和拨款，在前一批拨款期间总计超过200万美元。年度预算 的MSR是697,706美元，而CCSG的请求是82,192美元。因此，MSR只寻求11.8%的预算 CCSG基金的支持。

PROJECT SUMMARY – MICROSCOPY SHARED RESOURCE (MSR) The MSR provides timely and high-quality services to support OSUCCC investigators with access to instrument, technical advice and training support on a variety of sophisticated microscopy approaches. The MSR instrumentation is provided in a centrally-organized resource and all the equipment is available to OSUCCC membership with training. At the last review, the MSR was part of the Diagnostics Shared Resource Group, which was rated as Outstanding with minor comments about leadership and usage that have been addressed. During the current grant cycle, there was a significant investment in acquiring electron microscopy (cryogenic, transmission and scanning electron microscopes) and confocal live cell imaging, including enhanced super- resolution (SIM, STORM, TIRF) light microscopy capabilities funded through an NIH S10 equipment grant. The Specific Aims of the MSR are to: 1) provide a means for OSUCCC research community to obtain publication quality, higher resolution images of their cancer research for use in manuscripts and grant applications; 2) provide training to investigators for independent use of the instruments following training; 3) provide education and consultation opportunities to OSUCCC researchers for improved sample preparation, imaging techniques and image analysis; and, as a Developing Aim to provide OSUCCC members access to cryo-electron microscopy (cryo-EM). For this Developing Aim, the OSCUCC contributed to the purchase of two instruments with sub- nanometer resolution of purified and cellular particles. During the five-year grant period, the MSR has supported 319 investigators (32% OSUCCC members), and provided 5,730 hours of service and 44,605 hours of instrument time (33.3% of which was to OSUCCC members). The MSR contributed to 192 publications (32 > 10 impact factor) and 8 NCI grants (2 K22s, 1 P01, 1 P50, 8 R01s, and 1 U01). The MSR will support the microscopy needs of all OSUCCC strategic priorities. Given the robust OSUCCC recruitment, demand for services and new technologies will increase and capacity will be expanded. To align with immuno-oncology needs, the MSR will be a regular member of the Immune Monitoring and Discovery Platform (IMDP), and two-photon microscopes will be updated. Separately, the MSR is developing cryo-FIB capability for CET studies, and it will develop correlative light and electron microscopy and real-time single molecule cellular imaging platforms in collaboration with the GEdSR. Last, the MSR and GSR has been awarded a new grant to provide imaging capabilities that supports cell enrichment and single cell sequencing. The MSR is supported by outstanding institutional resources obtained by leveraging extensive partnerships with the OSUCCC, OSU Colleges, the OSU Office of Research and grants from the State of Ohio, all totaling over $2M over the prior grant period. The annual budget of the MSR is $697,706, yet the CCSG request is $82,192. As such, the MSR seeks only 11.8% budgetary support from CCSG funds.