Shared Resource 11: Microscopy (MSR)

项目摘要-显微镜共享资源（MSR） MSR提供及时和高质量的服务，以支持OSUCCC调查人员使用仪器， 在各种复杂的显微镜方法方面提供技术咨询和培训支持。所述msr 仪器仪表在集中组织的资源中提供，所有设备都可供OSUCCC使用 会员培训。在上次审查中，MSR是诊断共享资源组的一部分， 这被评为优秀与轻微的意见，领导和使用已得到解决。 在目前的赠款周期内，有一个重大的投资，以获得电子显微镜（低温， 透射和扫描电子显微镜）和共聚焦活细胞成像，包括增强的超 分辨率（SIM，STORM，TIRF）光学显微镜能力通过NIH S10设备赠款资助。的 MSR的具体目标是：1）为OSUCCC研究社区提供获取出版物的方法 他们的癌症研究的高质量、更高分辨率图像，可用于手稿和资助申请; 2） 培训研究者独立使用仪器; 3）提供教育 为OSUCCC研究人员提供改进样品制备和成像技术的咨询机会 和图像分析;以及，作为发展目标，为OSUCCC成员提供低温电子显微镜 （cryo-EM）。为了实现这一发展目标，OSCUCC出资购买了两台仪器， 纳米分辨率的纯化和细胞颗粒。在五年的资助期内，MSR支持了 319名调查员（32%为OSUCCC成员），提供了5，730小时的服务和44，605小时的 仪器时间（其中33.3%是OSUCCC成员）。MSR贡献了192篇出版物（32> 10 影响因子）和8项NCI资助（2项K22、1项P01、1项P50、8项R 01和1项U01）。MSR将支持显微镜检查 所有OSUCCC战略优先事项的需求。鉴于OSUCCC招聘强劲，对服务的需求和新的 技术将增加，能力将扩大。为了满足免疫肿瘤学需求，MSR将 成为免疫监测和发现平台（IMDP）和双光子显微镜的正式成员 将被更新。另外，MSR正在开发用于CET研究的冷冻FIB能力， 相关的光学和电子显微镜和实时单分子细胞成像平台合作 在GedSR。最后，MSR和GSR获得了一项新的拨款，以提供成像能力， 支持细胞富集和单细胞测序。MSR得到了杰出机构的支持 通过利用与OSUCCC、OSU学院、OSU办公室的广泛合作伙伴关系获得的资源 来自俄亥俄州的研究和赠款，在前一个赠款期内总额超过200万美元。年度预算 MSR为697，706美元，而CCSG的要求为82，192美元。因此，MSR仅寻求11.8%的预算 CCSG基金的支持。

PROJECT SUMMARY – MICROSCOPY SHARED RESOURCE (MSR) The MSR provides timely and high-quality services to support OSUCCC investigators with access to instrument, technical advice and training support on a variety of sophisticated microscopy approaches. The MSR instrumentation is provided in a centrally-organized resource and all the equipment is available to OSUCCC membership with training. At the last review, the MSR was part of the Diagnostics Shared Resource Group, which was rated as Outstanding with minor comments about leadership and usage that have been addressed. During the current grant cycle, there was a significant investment in acquiring electron microscopy (cryogenic, transmission and scanning electron microscopes) and confocal live cell imaging, including enhanced super- resolution (SIM, STORM, TIRF) light microscopy capabilities funded through an NIH S10 equipment grant. The Specific Aims of the MSR are to: 1) provide a means for OSUCCC research community to obtain publication quality, higher resolution images of their cancer research for use in manuscripts and grant applications; 2) provide training to investigators for independent use of the instruments following training; 3) provide education and consultation opportunities to OSUCCC researchers for improved sample preparation, imaging techniques and image analysis; and, as a Developing Aim to provide OSUCCC members access to cryo-electron microscopy (cryo-EM). For this Developing Aim, the OSCUCC contributed to the purchase of two instruments with sub- nanometer resolution of purified and cellular particles. During the five-year grant period, the MSR has supported 319 investigators (32% OSUCCC members), and provided 5,730 hours of service and 44,605 hours of instrument time (33.3% of which was to OSUCCC members). The MSR contributed to 192 publications (32 > 10 impact factor) and 8 NCI grants (2 K22s, 1 P01, 1 P50, 8 R01s, and 1 U01). The MSR will support the microscopy needs of all OSUCCC strategic priorities. Given the robust OSUCCC recruitment, demand for services and new technologies will increase and capacity will be expanded. To align with immuno-oncology needs, the MSR will be a regular member of the Immune Monitoring and Discovery Platform (IMDP), and two-photon microscopes will be updated. Separately, the MSR is developing cryo-FIB capability for CET studies, and it will develop correlative light and electron microscopy and real-time single molecule cellular imaging platforms in collaboration with the GEdSR. Last, the MSR and GSR has been awarded a new grant to provide imaging capabilities that supports cell enrichment and single cell sequencing. The MSR is supported by outstanding institutional resources obtained by leveraging extensive partnerships with the OSUCCC, OSU Colleges, the OSU Office of Research and grants from the State of Ohio, all totaling over $2M over the prior grant period. The annual budget of the MSR is $697,706, yet the CCSG request is $82,192. As such, the MSR seeks only 11.8% budgetary support from CCSG funds.