Post-translational control of cell fate decision

破译细胞命运决定的多层调控是具有挑战性的。虽然在理解转录调控的作用方面取得了进展，但对翻译后修饰的影响知之甚少。神经前体细胞表达发育下调蛋白4 （NEDD4）介导的泛素化对于性别决定是绝对必要的，这是一种独特的细胞命运决定模型，性腺细胞前体细胞沿着雄性或雌性途径分化。因此，本项目旨在详细分析需要NEDD4作用的哪个阶段以及哪些是关键的靶蛋白。这个项目可以提供更深入的理解翻译后修饰如何影响胚胎发生过程中细胞命运的决定。

Deciphering the multi-layered regulation of cell fate decisions is challenging. While progress has been made in understanding the role of transcriptional regulation, the influence of post-translational modification is poorly understood. Neural precursor cell expressed developmentally down-regulated protein 4 (NEDD4)-mediated ubiquitination is absolute necessary for sex determination, a unique model of cell fate decision where gonadal cell precursors differentiate either along the male or the female pathway. Thus, this project aims to analyse in detail at which stage NEDD4 action is required and what are the crucial target proteins. This project could provide a deeper understanding of how post-translational modifications influence cell fate decisions during embryogenesis.