Post-translational control of cell fate decision

细胞命运决定的翻译后控制

• 批准号: DP150101448
• 负责人: A/Prof Dagmar Wilhelm
• 金额: $ 35.18万
• 依托单位: The University of Melbourne
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2015
• 资助国家: 澳大利亚
• 起止时间: 2015-01-01 至 2017-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP150101448
• 关键词: Post; translational; control; cell; fate

Deciphering the multi-layered regulation of cell fate decisions is challenging. While progress has been made in understanding the role of transcriptional regulation, the influence of post-translational modification is poorly understood. Neural precursor cell expressed developmentally down-regulated protein 4 (NEDD4)-mediated ubiquitination is absolute necessary for sex determination, a unique model of cell fate decision where gonadal cell precursors differentiate either along the male or the female pathway. Thus, this project aims to analyse in detail at which stage NEDD4 action is required and what are the crucial target proteins. This project could provide a deeper understanding of how post-translational modifications influence cell fate decisions during embryogenesis.

破译细胞命运决定的多层次规则是具有挑战性的。虽然在理解转录调控的作用方面已经取得了进展，但对翻译后修饰的影响却知之甚少。神经前体细胞表达发育下调蛋白4(NEDD4)介导的泛素化对于性别决定是绝对必要的，这是一种独特的细胞命运决定模型，性腺细胞前体沿着男性或女性途径分化。因此，该项目旨在详细分析在哪个阶段需要NEDD4作用，以及关键的目标蛋白是什么。该项目可以提供对翻译后修饰如何影响胚胎发育过程中细胞命运决定的更深层次的理解。