Paper-based HIV self test

纸质 HIV 自检

基本信息

  • 批准号:
    10555209
  • 负责人:
  • 金额:
    $ 45.44万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2022
  • 资助国家:
    美国
  • 起止时间:
    2022-01-25 至 2024-12-31
  • 项目状态:
    已结题

项目摘要

Abstract: Current self-tests for HIV rely on detection of antibodies to the virus, and thus are not useful during the early stages of infection. These assays are also not appropriate for patients on antiretroviral therapy who may be at risk for viral rebound, as pre-existing anti-HIV antibodies mask changes in viral load. Conversely, current HIV diagnostics based on detection of viral RNA are sensitive, but too complex and expensive to be appropriate for self-testing. To address this critical need, we will develop the first enzyme-free sample-to-answer HIV viral load self-test, in which a fingerstick volume of blood is passively processed leading to amplification of the isolated HIV genomic RNA. The RNA amplification strategy will rely on hairpin cascade reactions (HCR), a DNA nanotechnology approach with proven single-copy sensitivity in other contexts, in a sessile droplet implementation. The entire assay will be implemented in an inexpensive, disposable, paper-based consumable. In the R61 phase, we propose to: (1) building on methods we have previously validated, optimize passive processing of whole blood coupled with paper-based viral lysis and RNA isolation; (2) complete development of HCR-based amplification of HIV genomic RNA; (3) integrate sample processing and RNA amplification into a single consumable; and (4) obtain feedback on assay design and usability from patients at risk for HIV infection, and develop a pilot manufacturing plan. In the R33 phase, we will (1) scale production of the assay with contract manufacturers in quantities suitable for pilot testing, and (2) complete testing of the assay with a cohort of 1000 patients at-risk for HIV infection, recruited from patients visiting the Emergency Department at the University of Rochester Medical Center.
摘要:目前的艾滋病毒自我检测依赖于病毒抗体的检测,因此不是

项目成果

期刊论文数量(0)
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会议论文数量(0)
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Benjamin L Miller其他文献

A patch for a splice
一个用于接头的补片
  • DOI:
    10.1038/nchembio.1839
  • 发表时间:
    2015-06-17
  • 期刊:
  • 影响因子:
    13.700
  • 作者:
    Benjamin L Miller
  • 通讯作者:
    Benjamin L Miller

Benjamin L Miller的其他文献

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{{ truncateString('Benjamin L Miller', 18)}}的其他基金

Paper-based HIV self test
纸质 HIV 自检
  • 批准号:
    10373830
  • 财政年份:
    2022
  • 资助金额:
    $ 45.44万
  • 项目类别:
Osteoimmunology Core
骨免疫学核心
  • 批准号:
    10402965
  • 财政年份:
    2017
  • 资助金额:
    $ 45.44万
  • 项目类别:
RNA Targeted Small Molecules: Connecting Binding Kinetics to Sequence Selectivity
RNA 靶向小分子:将结合动力学与序列选择性联系起来
  • 批准号:
    8550102
  • 财政年份:
    2012
  • 资助金额:
    $ 45.44万
  • 项目类别:
RNA Targeted Small Molecules: Connecting Binding Kinetics to Sequence Selectivity
RNA 靶向小分子:将结合动力学与序列选择性联系起来
  • 批准号:
    8737913
  • 财政年份:
    2012
  • 资助金额:
    $ 45.44万
  • 项目类别:
RNA Targeted Small Molecules: Connecting Binding Kinetics to Sequence Selectivity
RNA 靶向小分子:将结合动力学与序列选择性联系起来
  • 批准号:
    8914807
  • 财政年份:
    2012
  • 资助金额:
    $ 45.44万
  • 项目类别:
RNA Targeted Small Molecules: Connecting Binding Kinetics to Sequence Selectivity
RNA 靶向小分子:将结合动力学与序列选择性联系起来
  • 批准号:
    8259290
  • 财政年份:
    2012
  • 资助金额:
    $ 45.44万
  • 项目类别:
Preclinical Development of Lead Compounds Targeting Myotonic Dystrophy
针对强直性肌营养不良的先导化合物的临床前开发
  • 批准号:
    7963320
  • 财政年份:
    2010
  • 资助金额:
    $ 45.44万
  • 项目类别:
Preclinical Development of Lead Compounds Targeting Myotonic Dystrophy
针对强直性肌营养不良的先导化合物的临床前开发
  • 批准号:
    8109327
  • 财政年份:
    2010
  • 资助金额:
    $ 45.44万
  • 项目类别:
Photonic structures for direct ultrasensitive virus detection
用于直接超灵敏病毒检测的光子结构
  • 批准号:
    8029557
  • 财政年份:
    2009
  • 资助金额:
    $ 45.44万
  • 项目类别:
Photonic structures for direct ultrasensitive virus detection
用于直接超灵敏病毒检测的光子结构
  • 批准号:
    8238366
  • 财政年份:
    2009
  • 资助金额:
    $ 45.44万
  • 项目类别:
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