CONTROL AND EXPRESSION OF TYPE 1 PILI IN E COLI

大肠杆菌中 1 型 PILI 的控制和表达

• 批准号: 2061758
• 负责人: PAUL Edwin ORNDORFF
• 金额: $ 11.7万
• 依托单位: NORTH CAROLINA STATE UNIVERSITY RALEIGH
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-04-01 至 2000-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2061758
• 关键词: Escherichia coli; SDS polyacrylamide gel electrophoresis; autoradiography; bacterial genetics; communicable disease transmission; erythrocytes; gene expression; gene induction /repression; genetic manipulation; genetic recombination; genetic regulation; host organism interaction; intermolecular interaction; laboratory mouse; macrophage; mannose; microorganism culture; mutant; nucleic acid sequence; pilin; pilus; receptor binding; site directed mutagenesis

Type 1 pili of E. coli mediate adhesion to a variety of eukaryotic cells and the investigator has collaborated in studies that show that their major role in "virulence" is to promote communicability of E. coli from animal to animal. The PI's research focuses on the way that type 1 pili interact with eucaryotic cells and how certain minor pilus components influence pilus biogenesis. The intent is to enable intervention in colonization and spread of pathogenic bacteria and to learn more about mechanisms of assembly of polymerized supramolecular structures in general. Specific aims are 1. Examine the molecular interactions in receptor binding and 2. examine molecular interactions in pilus assembly.

大肠杆菌的1型菌毛介导对多种真核细胞的粘附