CONTROL AND EXPRESSION OF TYPE 1 PILI IN E COLI

大肠杆菌中 1 型 PILI 的控制和表达

• 批准号: 2886482
• 负责人: PAUL Edwin ORNDORFF
• 金额: $ 13.19万
• 依托单位: NORTH CAROLINA STATE UNIVERSITY RALEIGH
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-04-01 至 2001-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2886482
• 关键词: Escherichia coli; SDS polyacrylamide gel electrophoresis; autoradiography; bacterial genetics; communicable disease transmission; erythrocytes; gene expression; gene induction /repression; genetic manipulation; genetic recombination; genetic regulation; host organism interaction; intermolecular interaction; laboratory mouse; macrophage; mannose; microorganism culture; mutant; nucleic acid sequence; pilin; pilus; receptor binding; site directed mutagenesis

Type 1 pili of E. coli mediate adhesion to a variety of eukaryotic cells and the investigator has collaborated in studies that show that their major role in "virulence" is to promote communicability of E. coli from animal to animal. The PI's research focuses on the way that type 1 pili interact with eucaryotic cells and how certain minor pilus components influence pilus biogenesis. The intent is to enable intervention in colonization and spread of pathogenic bacteria and to learn more about mechanisms of assembly of polymerized supramolecular structures in general. Specific aims are 1. Examine the molecular interactions in receptor binding and 2. examine molecular interactions in pilus assembly.

大肠杆菌1型菌毛介导与多种真核细胞的黏附 研究人员合作的研究表明，他们的 在“毒力”中的主要作用是促进大肠杆菌从 动物对动物。PI的研究重点是类型1毛发的方式 与真核细胞的相互作用以及某些微小的菌毛成分如何 影响菌毛的生物发生。这样做的目的是为了在 病原菌的定植和传播，并了解更多关于 聚合型超分子结构的组装机理 将军。具体目标是1.检查分子相互作用 受体结合和2.检查菌毛组装中的分子相互作用。

项目成果

Effect of type 1 piliation on in vitro killing of Escherichia coli by mouse peritoneal macrophages.

1 型毛毛作用对小鼠腹腔巨噬细胞体外杀灭大肠杆菌的影响。

• DOI: 10.1128/iai.58.10.3448-3454.1990
• 发表时间: 1990
• 期刊: Infection and immunity
• 影响因子: 3.1
• 作者: Keith,BR;Harris,SL;Russell,PW;Orndorff,PE
• 通讯作者: Orndorff,PE

Receptor-binding function of type 1 pili effects bladder colonization by a clinical isolate of Escherichia coli.

1 型菌毛的受体结合功能影响临床分离的大肠杆菌在膀胱的定植。

• DOI: 10.1128/iai.53.3.693-696.1986
• 发表时间: 1986
• 期刊: Infection and immunity
• 影响因子: 3.1
• 作者: Keith,BR;Maurer,L;Spears,PA;Orndorff,PE
• 通讯作者: Orndorff,PE

Two modes of control of pilA, the gene encoding type 1 pilin in Escherichia coli.

pilA（大肠杆菌中编码 1 型菌毛蛋白的基因）的两种控制模式。

• DOI: 10.1128/jb.164.1.321-330.1985
• 发表时间: 1985
• 期刊: Journal of bacteriology
• 影响因子: 3.2
• 作者: Orndorff,PE;Spears,PA;Schauer,D;Falkow,S
• 通讯作者: Falkow,S

Impaired colonization by and full invasiveness of Escherichia coli K1 bearing a site-directed mutation in the type 1 pilin gene.

带有 1 型菌毛蛋白基因定点突变的大肠杆菌 K1 的定植和完全侵袭性受损。

• DOI: 10.1128/iai.58.1.275-278.1990
• 发表时间: 1990
• 期刊: Infection and immunity
• 影响因子: 3.1
• 作者: Bloch,CA;Orndorff,PE
• 通讯作者: Orndorff,PE

Metastable regulation of type 1 piliation in Escherichia coli and isolation and characterization of a phenotypically stable mutant.

大肠杆菌中 1 型纤毛作用的亚稳态调节以及表型稳定突变体的分离和表征。

• DOI: 10.1128/jb.168.1.179-185.1986
• 发表时间: 1986
• 期刊: Journal of bacteriology
• 影响因子: 3.2
• 作者: Spears,PA;Schauer,D;Orndorff,PE
• 通讯作者: Orndorff,PE