Magnetic Antimicrobial Peptide-based (MAP) DNA Extraction Kit

磁性抗菌肽 (MAP) DNA 提取试剂盒

• 批准号: 103321
• 金额: $ 7.17万
• 依托单位: LIG BIOWISE LIMITED
• 依托单位国家: 英国
• 项目类别: Feasibility Studies
• 财政年份: 2017
• 资助国家: 英国
• 起止时间: 2017 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=103321
• 关键词: Magnetic; Antimicrobial; Peptide; based; MAP

The accuracy and sensitivity of a molecular diagnostic method is dependent of the efficient extraction of nucleic acids and absence of amplification inhibitors in the reaction mixture. Bacterial DNA/RNA extraction usually requires enzymatic, physical or chemical cell lysis, which is especially critical when working with Gram + bacteria. DNA/RNA purification methods are then applied to remove amplification inhibitors. Additionally, molecular diagnostic tests that require the detection of low concentrations of the target bacteria frequently comprise an extra laborious bacterial enrichment step. LIG Biowise is currently developing a Magnetic Antimicrobial Peptide-based (MAP) DNA Extraction method. This method will combine the steps of bacterial enrichment and DNA extraction and purification in a rapid and simple procedure, which will be compatible with a variety of clinical and environmental samples. This enhanced performance DNA extraction method will be developed both as a stand-alone kit and as a step that can be incorporated in clinical and food pathogen diagnostic tests to increase its accuracy, sensitivity and speed.

分子诊断方法的准确性和灵敏度取决于核酸的有效提取和反应混合物中扩增抑制剂的不存在。细菌DNA/RNA提取通常需要酶促、物理或化学细胞裂解，这在处理革兰氏阳性细菌时尤为关键。然后应用DNA/RNA纯化方法以去除扩增抑制剂。另外，需要检测低浓度的靶细菌的分子诊断测试通常包括额外费力的细菌富集步骤。LIG Biowise目前正在开发一种基于磁性抗菌肽（MAP）的DNA提取方法。该方法将联合收割机的细菌富集和DNA提取和纯化的步骤结合在一个快速和简单的过程中，这将是兼容的各种临床和环境样品。这种增强性能的DNA提取方法将被开发为一个独立的试剂盒，并作为一个步骤，可以纳入临床和食品病原体诊断测试，以提高其准确性，灵敏度和速度。