Magnetic Antimicrobial Peptide-based (MAP) DNA Extraction Kit

分子诊断方法的准确性和灵敏度取决于核酸的有效提取和反应混合物中没有扩增抑制剂。细菌DNA/RNA提取通常需要酶法、物理或化学细胞裂解，这在处理革兰氏阳性细菌时尤其关键。然后用DNA/RNA纯化方法去除扩增抑制物。此外，需要检测低浓度目标细菌的分子诊断测试通常包括额外费力的细菌浓缩步骤。Lig Biowise目前正在开发一种基于磁性抗菌肽(MAP)的DNA提取方法。该方法将细菌浓缩和DNA提取纯化的步骤结合在一起，快速简便，适用于多种临床和环境样品。这种性能增强的DNA提取方法将作为一个独立的试剂盒开发，并作为一个步骤可以纳入临床和食品病原体诊断测试，以提高其准确性、灵敏度和速度。

The accuracy and sensitivity of a molecular diagnostic method is dependent of the efficient extraction of nucleic acids and absence of amplification inhibitors in the reaction mixture. Bacterial DNA/RNA extraction usually requires enzymatic, physical or chemical cell lysis, which is especially critical when working with Gram + bacteria. DNA/RNA purification methods are then applied to remove amplification inhibitors. Additionally, molecular diagnostic tests that require the detection of low concentrations of the target bacteria frequently comprise an extra laborious bacterial enrichment step. LIG Biowise is currently developing a Magnetic Antimicrobial Peptide-based (MAP) DNA Extraction method. This method will combine the steps of bacterial enrichment and DNA extraction and purification in a rapid and simple procedure, which will be compatible with a variety of clinical and environmental samples. This enhanced performance DNA extraction method will be developed both as a stand-alone kit and as a step that can be incorporated in clinical and food pathogen diagnostic tests to increase its accuracy, sensitivity and speed.