PROTEOGLYCAN STRUCTURE, METABOLISM AND ROLE IN TENDON
PROTEOGLYCAN STRUCTURE, METABOLISM AND ROLE IN TENDON
批准号:
2079126
负责人:
KATHRYN G VOGEL
金额:
$18.79万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1985
资助国家:
美国
项目状态:
已结题
起止时间:
1985-09-23 至 1998-12-31
关键词:
animal tissue antibody calcium flux cell type confocal scanning microscopy cytoskeleton enzyme activity extracellular matrix gene expression glycoprotein biosynthesis glycoprotein structure immunocytochemistry in situ hybridization mechanical stress messenger RNA phosphorylation protein metabolism protein structure function protein tyrosine phosphatase proteoglycan tendons tissue /cell culture transforming growth factors western blottings
中文摘要
这个项目的长期目标是了解结构,
一类细胞外基质的代谢、调节及其作用
肌腱中的组成成分--蛋白多糖。肌腱通常只包含
少量的小蛋白多糖粘附素。然而,一只蜗牛-
在牛深屈肌腱通过的地方形成丰富的纤维软骨
在骨骼下,在原位经历压缩和拉伸。在……里面
体外实验支持这样的假设,即机械负荷导致
增加大蛋白多糖的合成和积累,产生
具有特定需要的附加压缩硬度的组织
机械环境。建议进行实验,以研究长时间的
肌腱细胞对施加力的长期和短期反应
比机械载荷。(1)将建造一个新的设备以加载
体外正常拉伸的胎牛肌腱的离散区
一段时间内的应力、法向压应力或剪应力
从几分钟到几天不等。不同载荷作用下的影响
环境对蛋白多糖合成量和类型的影响,其
结构和周转,以及对mRNA的表达和定位
几种基质成分,将被测定。合成与活化
将测量作为加载结果的转化生长因子-β的含量。(2)共焦
显微镜将被用来可视化细胞对
组织负荷,如(Ca++)的变化或组织的改变
细胞骨架。酪氨酸磷酸化或酪氨酸的活性
磷酸酶将在不同的负荷方案后进行评估。(3)
细胞外基质组织和特定基质成分的作用
在调节中,细胞对组织负荷的反应将被确定
利用酶改变基质完整性和药物改变细胞骨架
组织。这一综合实验计划将首次,
允许将不同的测量机械载荷与相关的
一个组织中的细胞反应。纤维软骨提供压缩
刚性和滑动面的精确位置
属性对肌腱很有用。不适当的发展
纤维软骨导致与重复运动相关的病理
症状、愈合特性改变和风湿病。这些研究
这一提议将增加对组织水平反应的理解
使软结缔组织能够适当地调节其
组合以适应不断变化的机械环境。
英文摘要
The long-term goal of this project is to understand the structure,
metabolism, regulation, and roles of one category of extracellular matrix
constituent in tendon - the proteoglycans. Tendon generally contains only
a small amount of the small proteoglycan decorin. However, an aggrecan-
rich fibrocartilage develops where the bovine deep flexor tendon passes
under bone and experiences compression as well as tension in situ. In
vitro experiments support the hypothesis that mechanical loading leads to
increased synthesis and accumulation of large proteoglycans, generating a
tissue with the added compressive stiffness needed for its particular
mechanical environment. Experiments are proposed to investigate the long-
term and short-term responses of cells in tendon to the imposition of
specific mechanical loads . (1) A new device will be constructed to load
discrete regions of a fetal bovine tendon in vitro with normal tensile
stress, normal compressive stress, or shear stress, for time periods
ranging from minutes to days. The effects of different loading
environments on the amount and type of proteoglycan synthesized, its
structure and turnover, as well as on mRNA expression and localization for
several matrix constituents, will be determined. Synthesis and activation
of TGF-Beta as a result of loading will be measured. (2) Confocal
microscopy will be used to visualize immediate cellular responses to
tissue loading, such as changes in (Ca++] or altered organization of the
cytoskeleton. The activity of tyrosine phosphorylation or tyrosine
phosphatase will be assessed after different loading regimens. (3) The
role of extracellular matrix organization and specific matrix components
in regulation the cellular response to tissue loading will be determined
using enzymes to alter matrix integrity and drugs to alter cytoskeletal
organization. This integrated experimental plan will, for the first time,
allow correlation of distinct measured mechanical loads with relevant
cellular responses in one tissue. Fibrocartilage provides compressive
stiffness and a gliding surface in precisely the location where such
properties are useful to the tendon. The inappropriate development of
fibrocartilage leads to pathology associated with repetitive motion
syndromes, altered healing properties, and rheumatic disease. The studies
of this proposal will increase understanding of tissue-level responses
that allow soft connective tissues to appropriately modulate their
composition to meet a changing mechanical environment.
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UNDERGRAD BIOMED RESEARCH TRAINING - UNIV OF NEW MEXICO
-
批准号:7097853
-
项目类别:
-
资助金额:$11.84万
-
财政年份:2000
-
负责人:KATHRYN G VOGEL
-
依托单位:
UNDERGRAD BIOMED RESEARCH TRAINING - UNIV OF NEW MEXICO
-
批准号:6769418
-
项目类别:
-
资助金额:$6.48万
-
财政年份:2000
-
负责人:KATHRYN G VOGEL
-
依托单位:
PROTEOGLYCAN STRUCTURE, METABOLISM AND ROLE IN TENDON
-
批准号:2079127
-
项目类别:
-
资助金额:$1.08万
-
财政年份:1995
-
负责人:KATHRYN G VOGEL
-
依托单位:
SMALL INSTRUMENTATION GRANT
-
批准号:3523116
-
项目类别:
-
资助金额:$2.06万
-
财政年份:1992
-
负责人:KATHRYN G VOGEL
-
依托单位:
SMALL INSTRUMENTATION PROGRAM
-
批准号:3523037
-
项目类别:
-
资助金额:$0.98万
-
财政年份:1988
-
负责人:KATHRYN G VOGEL
-
依托单位:
SPECTROPHOTOMETER AND ACCESSORIES
-
批准号:3523023
-
项目类别:
-
资助金额:$0.93万
-
财政年份:1987
-
负责人:KATHRYN G VOGEL
-
依托单位:
PROTEOGLYCAN STRUCTURE, METABOLISM AND ROLE IN TENDON
-
批准号:3157476
-
项目类别:
-
资助金额:$11.96万
-
财政年份:1985
-
负责人:KATHRYN G VOGEL
-
依托单位:
PROTEOGLYCAN STRUCTURE, METABOLISM AND ROLE IN TENDON
-
批准号:3157480
-
项目类别:
-
资助金额:$11.2万
-
财政年份:1985
-
负责人:KATHRYN G VOGEL
-
依托单位:
PROTEOGLYCAN STRUCTURE, METABOLISM AND ROLE IN TENDON
-
批准号:3157477
-
项目类别:
-
资助金额:$17.18万
-
财政年份:1985
-
负责人:KATHRYN G VOGEL
-
依托单位:
FIBROBLAST PROTEOGLYCANS AND CONNECTIVE TISSUE MATRIX
-
批准号:3116197
-
项目类别:
-
资助金额:$7.82万
-
财政年份:1985
-
负责人:KATHRYN G VOGEL
-
依托单位:
PROTEOGLYCAN STRUCTURE, METABOLISM AND ROLE IN TENDON
-
批准号:3157483
-
项目类别:
-
资助金额:$17.42万
-
财政年份:1985
-
负责人:KATHRYN G VOGEL
-
依托单位:
PROTEOGLYCAN STRUCTURE, METABOLISM AND ROLE IN TENDON
-
批准号:2079128
-
项目类别:
-
资助金额:$17.39万
-
财政年份:1985
-
负责人:KATHRYN G VOGEL
-
依托单位:
FIBROBLAST PROTEOGLYCANS AND CONNECTIVE TISSUE MATRIX
-
批准号:3116198
-
项目类别:
-
资助金额:$6.27万
-
财政年份:1985
-
负责人:KATHRYN G VOGEL
-
依托单位:
PROTEOGLYCAN STRUCTURE, METABOLISM AND ROLE IN TENDON
-
批准号:3154455
-
项目类别:
-
资助金额:$12.0万
-
财政年份:1985
-
负责人:KATHRYN G VOGEL
-
依托单位:
PROTEOGLYCAN STRUCTURE, METABOLISM AND ROLE IN TENDON
-
批准号:2006126
-
项目类别:
-
资助金额:$18.07万
-
财政年份:1985
-
负责人:KATHRYN G VOGEL
-
依托单位:
PROTEOGLYCAN STRUCTURE, METABOLISM AND ROLE IN TENDON
-
批准号:2079124
-
项目类别:
-
资助金额:$17.77万
-
财政年份:1985
-
负责人:KATHRYN G VOGEL
-
依托单位:
FIBROBLAST PROTEOGLYCANS AND CONNECTIVE TISSUE MATRIX
-
批准号:3116196
-
项目类别:
-
资助金额:$7.86万
-
财政年份:1985
-
负责人:KATHRYN G VOGEL
-
依托单位:
PROTEOGLYCAN STRUCTURE, METABOLISM AND ROLE IN TENDON
-
批准号:3157482
-
项目类别:
-
资助金额:$16.81万
-
财政年份:1985
-
负责人:KATHRYN G VOGEL
-
依托单位:
PROTEOGLYCAN STRUCTURE, METABOLISM AND ROLE IN TENDON
-
批准号:3157481
-
项目类别:
-
资助金额:$15.72万
-
财政年份:1985
-
负责人:KATHRYN G VOGEL
-
依托单位:
PROTEOGLYCAN STRUCTURE, METABOLISM AND ROLE IN TENDON
-
批准号:2633638
-
项目类别:
-
资助金额:$18.79万
-
财政年份:1985
-
负责人:KATHRYN G VOGEL
-
依托单位:
海外基金