FIBROBLAST PROTEOGLYCANS AND CONNECTIVE TISSUE MATRIX

成纤维细胞蛋白聚糖和结缔组织基质

• 批准号: 3116197
• 负责人: KATHRYN G VOGEL
• 金额: $ 7.82万
• 依托单位: UNIVERSITY OF NEW MEXICO
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-01-01 至 1987-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3116197
• 关键词: Ehlers Danlos syndrome; connective tissue metabolism; electron microscopy; extracellular matrix; fibroblasts; gel filtration chromatography; human subject; human tissue; immunofluorescence technique; ion exchange chromatography; mucopolysaccharidosis type IV; osteogenesis imperfecta; proteoglycan; tissue /cell culture

Connective tissue is a complex of several macromolecules, each with its own specific inherited molecular characteristics and biosynthetic processing. A molecular defect in one component of the matrix could lead to defective interactions at many levals either because specific binding between extracellular molecules has been altered or because feedback regulation of biosynthesis has resulted in alterations of the intracellular processing and extracellular quantity for other matrix components. The hypothesis to be tested in these experiments is that altered collagen metabolism results in secondary alterations in proteoglycan metabolism, producing structural and functional changes in the connective tissue complex. In vitro experiments have demonstrated that proteoglycan type and concentration can specifically influence collagen fibrillogenesis. The quantity and type of proteoglycans produced by normal adult dermal explants and skin fibroblast cell lines established from these explants will be determined, in order to provide the baseline for these investigations. The quantity, type, localization, labeling kinetics and turnover of proteoglycans produced by these normal cells will be compared with similar determinations for cells from patients for whom a specific defect in type I or type III collagen structure and metabolism has been associated with decreased secretion of that collagen and connective tissue disease (osteogenesis imperfecta and Ehlers-Danlos syndrome, respectively). The morphology of the insoluble matrix produced by these cells in culture will be described by immunofluorescence microscopy and by electron microscopy. In addition, proteoglycan metabolism will be examined in dermal explant cultures from normal and affected individuals in order to determine whether the alterations noticed in culture are also found in cells in their tissue environment. If no differences are found it will mean that the biosynthetic and transport pathways for collagen and proteoglycans are separate, producing an altered extracellular ratio for these macromolecules that could influence matrix structure. If proteoglycan metabolism is altered in cells with the collagen defects, some type of co-regulation of these molecules will be indicated. Increased understanding of the relationship between collagen and proteoglycans in regulating the formation of a functional connective tissue matrix will be useful in understanding the alterations of connective tissue that accompany much more common conditions, such as normal aging.

结缔组织是几个大分子的复合体，每个大分子都有自己的特点