CD6--TRANSCRIPTIONAL REGULATION & CELLULAR INTERACTIONS

CD6--转录调控

• 批准号: 2077553
• 负责人: NORA SINGER
• 金额: $ 9.07万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-04-01 至 2000-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2077553
• 关键词: CD antigens; CD3 molecule; T lymphocyte; antigen presenting cell; autoimmunity; cell adhesion; cell adhesion molecules; cell cell interaction; genetic library; genetic transcription; human tissue; immunopathology; keratinocyte; monoclonal antibody; nucleic acid hybridization; nucleic acid probes; protein structure function; receptor binding; rheumatoid arthritis; southern blotting; thin layer chromatography; transfection

This application proposes funding with the specific intent of developing an independent research program by the principal investigator. The applicant has received clinical training in the fields of pediatrics and internal medicine and is a board certified internist and pediatrician. She is currently completing subspecialty training in the fields of pediatric rheumatology and adult rheumatology and has pursued her interest in basic science research in the areas of T cell immunology and autoimmune disease. This training is in preparation of an academic career as a rheumatologist/immunologist with a special interest in autoimmune diseases of children and young adults, and their immunopathogenesis. This research proposal examines the role of CD6, a glycoprotein expressed on T lymphocytes, in autoreactivity and in cell-cell interactions, including binding of antigen presenting cells in peripheral blood, T cells. Experiments are also proposed to examine the regulation of CD6. This investigator's previous work on CD6, suggests the existence of a CD6 ligand on antigen presenting cells in peripheral blood on synoviocytes and on keratinocytes. This applicant has demonstrated that anti-CD6 monoclonal antibodies inhibit both antigen specific and autoreactive response of human cloned T lymphocytes, as well as binding of CD6+ cells to not-T cells. This is vitro model of autoreactivity appears to have relevance to human autoimmunity. The applicant is now in a position to develop her own independent research program as a junior faculty member of the Department of Internal Medicine, with continuing connections to the Department of Pediatrics. It is expected that the applicant will be promoted to a tenure track junior faculty position in the Departments of Pediatrics and Internal Medicine during the time of the award. The sponsor, who is the head of the Division of Rheumatology, is committed to contributing protected time and resources to the applicant. Additionally the proposed collaborators have demonstrated expertise in the proposed areas of research and in the training of investigators. The applicant's long term goals are to participate in an academic medical community as a rheumatologist with a combined appointment in both a Department of Pediatrics and Internal Medicine. Furthermore, as a basic scientist she hopes to study areas of basic immunology and molecular genetics in order to advance understanding of autoimmune disease. Eventually, she hopes to provide a basic science laboratory environment for pediatric rheumatology trainees in order to contribute to knowledge in areas relevant to pediatric rheumatic diseases.

本申请提出的资金与发展的具体意图 一个独立的研究计划由首席研究员。的 申请人接受过儿科领域的临床培训， 内科，是一个委员会认证的内科医生和儿科医生。 她目前正在完成以下领域的专科培训： 儿童风湿病学和成人风湿病学， 对T细胞免疫学领域的基础科学研究感兴趣， 自身免疫性疾病这项培训是为学术生涯做准备 作为一名对自身免疫性疾病特别感兴趣的风湿病学家/免疫学家， 儿童和年轻人的疾病及其免疫发病机制。 这项研究提案探讨了CD 6（一种表达的糖蛋白）的作用 在自身反应性和细胞间相互作用中， 包括外周血中抗原呈递细胞的结合，T 细胞还提出了实验来检查CD 6的调节。 这位研究者以前对CD 6的研究表明， 外周血抗原呈递细胞配体和滑膜细胞 和角质形成细胞。本申请人已证明抗CD 6 单克隆抗体抑制抗原特异性和自身反应性 人克隆T淋巴细胞的反应以及CD 6+细胞的结合 到非T细胞这是一个自身反应性的体外模型， 与人类自身免疫有关。 申请人现在能够发展自己的独立 研究计划作为内部部门的初级教员 医学，与儿科系保持联系。 预计申请人将晋升为终身教职 儿科和内科系的初级教员职位 获奖期间的医学。赞助商，谁是负责人， 流变学分部致力于贡献受保护的时间 资源给申请人。此外，建议的合作伙伴 在拟议的研究领域和 培训调查员。 申请人的长期目标是参加学术医疗 社区作为一个风湿病学家与联合任命， 儿科和内科。此外，作为基本 她希望研究基础免疫学和分子生物学领域的科学家， 遗传学，以促进对自身免疫性疾病的理解。 最终，她希望提供一个基本的科学实验室环境， 为儿科风湿病学培训生，以促进知识 在儿科风湿性疾病相关领域。