CD6--TRANSCRIPTIONAL REGULATION & CELLULAR INTERACTIONS

CD6--转录调控

• 批准号: 2077553
• 负责人: NORA SINGER
• 金额: $ 9.07万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-04-01 至 2000-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2077553
• 关键词: CD antigens; CD3 molecule; T lymphocyte; antigen presenting cell; autoimmunity; cell adhesion; cell adhesion molecules; cell cell interaction; genetic library; genetic transcription; human tissue; immunopathology; keratinocyte; monoclonal antibody; nucleic acid hybridization; nucleic acid probes; protein structure function; receptor binding; rheumatoid arthritis; southern blotting; thin layer chromatography; transfection

This application proposes funding with the specific intent of developing an independent research program by the principal investigator. The applicant has received clinical training in the fields of pediatrics and internal medicine and is a board certified internist and pediatrician. She is currently completing subspecialty training in the fields of pediatric rheumatology and adult rheumatology and has pursued her interest in basic science research in the areas of T cell immunology and autoimmune disease. This training is in preparation of an academic career as a rheumatologist/immunologist with a special interest in autoimmune diseases of children and young adults, and their immunopathogenesis. This research proposal examines the role of CD6, a glycoprotein expressed on T lymphocytes, in autoreactivity and in cell-cell interactions, including binding of antigen presenting cells in peripheral blood, T cells. Experiments are also proposed to examine the regulation of CD6. This investigator's previous work on CD6, suggests the existence of a CD6 ligand on antigen presenting cells in peripheral blood on synoviocytes and on keratinocytes. This applicant has demonstrated that anti-CD6 monoclonal antibodies inhibit both antigen specific and autoreactive response of human cloned T lymphocytes, as well as binding of CD6+ cells to not-T cells. This is vitro model of autoreactivity appears to have relevance to human autoimmunity. The applicant is now in a position to develop her own independent research program as a junior faculty member of the Department of Internal Medicine, with continuing connections to the Department of Pediatrics. It is expected that the applicant will be promoted to a tenure track junior faculty position in the Departments of Pediatrics and Internal Medicine during the time of the award. The sponsor, who is the head of the Division of Rheumatology, is committed to contributing protected time and resources to the applicant. Additionally the proposed collaborators have demonstrated expertise in the proposed areas of research and in the training of investigators. The applicant's long term goals are to participate in an academic medical community as a rheumatologist with a combined appointment in both a Department of Pediatrics and Internal Medicine. Furthermore, as a basic scientist she hopes to study areas of basic immunology and molecular genetics in order to advance understanding of autoimmune disease. Eventually, she hopes to provide a basic science laboratory environment for pediatric rheumatology trainees in order to contribute to knowledge in areas relevant to pediatric rheumatic diseases.

此应用程序建议提供资金，具体目的是开发 由首席调查员进行的独立研究计划。这个 申请人曾接受过儿科和其他领域的临床培训 内科医生，董事会认证的内科医生和儿科医生。 她目前正在完成以下领域的分专业培训 儿童风湿病和成人风湿病，并一直在追求她 对T细胞免疫学领域的基础科学研究和 自身免疫性疾病。这次培训是为学术生涯做准备。 作为一名对自身免疫特别感兴趣的风湿学家/免疫学家 儿童和青壮年疾病及其免疫发病机制。 这项研究计划考察了CD6的作用，CD6是一种表达的糖蛋白 在T淋巴细胞上，在自身反应和细胞-细胞相互作用中， 包括外周血中抗原提呈细胞的结合，T 细胞。还建议用实验来检验CD6的调节。 这位研究人员之前对CD6的研究表明，CD6的存在 滑膜细胞表面外周血中抗原提呈细胞的配体 角质形成细胞上也有。这位申请人已经证明了抗CD6 单抗既抑制抗原特异性又抑制自身反应 人克隆T淋巴细胞的反应及CD6+细胞的结合 到非T细胞。这是一种体外自体反应性模型 与人类自身免疫有关。 申请者现在可以发展自己的独立能力了 作为内务部初级教员的研究计划 医学，与儿科系有持续的联系。 预计申请者将被提升到终身教职轨道 初级教员在儿科和内科的职位 在获奖时间内的药物。赞助商，也就是 风湿科致力于贡献受保护的时间 和资源提供给申请者。此外，建议的合作者 在拟议的研究领域和在 对调查人员的培训。 申请者的长期目标是参加学术医学 社区作为风湿科医生，在两个国家和地区兼任 儿科和内科。此外，作为一个基本的 她希望研究基础免疫学和分子生物学领域的科学家 遗传学，以促进对自身免疫性疾病的了解。 最终，她希望提供一个基础的科学实验室环境 为儿科风湿病实习生贡献知识 在与儿科风湿病相关的领域。