ALTERNATIVE SPLICING OF CONTRACTILE PROTEIN GENES

收缩蛋白基因的选择性剪接

• 批准号: 2079165
• 负责人: Bernardo Nadal Ginard
• 金额: $ 1.94万
• 依托单位: BOSTON CHILDREN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-01-01 至 1995-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2079165
• 关键词: DNA binding protein; RNA splicing; cell free system; genetic regulatory element; genetic transcription; myosins; nucleic acid sequence; pyrimidines; sarcomeres; smooth muscle; transcription factor; tropomyosin; troponin

DESCRIPTION: (Adapted from the Investigator's abstract): The overall goal of the research proposed in this application continues to be the identification and characterization of cis- and trans-acting factors involved in the regulation of alternative splicing. To this end, the investigators will continue to use mini-gene constructs and particular sequence elements from the contractile protein genes alpha-tropomyosin and troponin T as a model system that will be analyzed using in vivo and in vitro cell-free splicing system. Three main specific aims will be pursued: 1.-To analyze and characterize the structure and mechanism of action of a splicing factor, Polypyrimidine Binding Protein (PBP), which they have recently purified and partially sequenced. This factor plays an important role in determining 3' splice strength and might be involved in the early steps of splice site commitment. 2.-To further determine the binding site of a negative splicing factor specific to smooth muscle cells. To isolate, purify, and characterize the mechanism of action of this factor that works by blocking the default splicing pattern. 3.-Using a highly selective genetic approach, to identify and characterize a positive splicing factor specific to mature smooth muscle cells which determines the regulated splicing pattern of the troponin T transcript. It is expected that the cloning and characterization of these three splicing factors will make a significant contribution to the understanding of both constitutive and alternative models of splicing.

描述：（改编自研究者摘要）：总体目标

项目成果

Catalytic activity of an RNA domain derived from the U6-U4 RNA complex.

源自 U6-U4 RNA 复合物的 RNA 结构域的催化活性。

• DOI: 10.1126/science.8272868
• 发表时间: 1994
• 期刊: Science (New York, N.Y.)
• 作者: Yang,JH;Cedergren,R;Nadal-Ginard,B
• 通讯作者: Nadal-Ginard,B