MECHANISM OF HORMONE ACTION ON CELLS IN CULTURE

乳腺癌是女性发病和死亡的主要原因。 35岁及以上。关于致癌过程的知识有限。 理解雌激素靶细胞增殖的控制是 对实施预防、预测、诊断和治疗至关重要 旨在降低这些妇女发病率和死亡率的措施。我们 采纳了扩散是一种构成的、占主导地位的属性的前提 后生动物的细胞。因此，我们研究的中心是 乳腺肿瘤特异性增殖抑制因子的鉴定 细胞。我们确定人血清白蛋白(HSA)就是这种类型的抑制物。 用重组人血清白蛋白验证了抑制作用的特异性。 关于靶细胞类型；在激素和生长因子中，只有 雌激素克服了这种抑制作用。此外，没有其他蛋白质被发现 发现会产生这种抑制作用。因为人的类比蛋白血症血清 也能抑制MCF7细胞的增殖，出现了一个悖论。 我们发现了与高蛋白血症血清假设一致的证据 含有截短的HSA多肽，因此，HSA可能是 被认为是一种“前激素”。我们将更好地解决这一悖论， 进一步阐明了人血清白蛋白和雌激素对血管生成的作用机制。 支持期间乳腺肿瘤细胞的增殖情况。

Breast cancer is a leading cause of morbidity and mortality among women age 35 and older. Knowledge on the carcinogenic process is limited. Understanding the control of the proliferation of estrogen target cells is crucial to implement preventive, prognostic, diagnostic and therapeutic measures aimed at reducing the morbidity and mortality in these women. We adopted the premise that proliferation is a constitutive, dominant property of cells in metazoa. Thus, the centerpiece of our research is the identification of specific inhibitors of the proliferation of breast tumor cells. We identified human serum albumin (HSA) as such type of inhibitor. Using recombinant HSA we verified that the inhibitory effect was specific regarding the target cell type; among hormones and growth factors, only estrogens overcame this inhibitory effect. Moreover, no other protein was found to produce this inhibitory effect. Because human analbuminemic serum is also able to inhibit the proliferation of MCF7 cells, a paradox emerged. We found evidence consistent with the hypothesis that analbuminemic serum contains as truncated HSA polypeptide, and therefore, HSA may may be considered as a "prohormone". We will better to resolve this paradox and shed further light on the mechanism of action of HSA and estrogens on the proliferation of breast tumor cells during the period of support.