MECHANISM OF HORMONE ACTION ON TARGET CELLS IN CULTURE

雌激素（E）敏感的人乳腺肿瘤细胞（MCF 7， T47 D）在“动物-文化”系统中的作用是研究的中心问题， 这个实验室 雌二醇-17 β（E2）对特定蛋白质 合成及其与这些E2 目标 细胞正在被鉴定。 这些细胞携带雌激素并繁殖 在裸鼠中，E2 比那些 E2 缺乏。 在培养条件下， 血清呈剂量依赖性抑制细胞增殖; 雌激素取消这种抑制。 这些数据和其他数据是兼容的 间接和消极控制核武器扩散的概念 E2- 敏感细胞。 这项赠款的具体目的是净化 特异性抑制E2- 敏感的人体细胞 （雌激素类），并确定三苯乙烯的影响 （“抗雌激素”）对上述细胞增殖的影响， 间接和直接消极控制范式的背景。 （丁）

The proliferation of estrogen (E)-sensitive human breast tumor cells (MCF7, T47D) in an "in animal-in culture" system is the central issue studied in this lab. The effect of estradiol-17 beta (E2) on specific protein synthesis and its relation with the proliferation of these E2 target cells is being characterized. These cells carry estrophilins and multiply faster in nude mice to which E2 is administered than in those in which E2 is lacking. In culture conditions, charcoal-dextran stripped human sera inhibit the proliferation of these cells in dose-dependent pattern; estrogens cancel this inhibition. These and additional data are compatible with the notion of an indirect and negative control of the proliferation of E2-sensitive cells. The specific aims of this grant are to purify the specific inhibitors of the proliferation of E2-sensitive human cells (estrocolyones) and determine the effect of triphenylethylenes ("antiestrogens") on the proliferation of the above mentioned cells within the context of the indirect and direct negative control paradigm. (D)