RATIONAL DRUG DESIGN BASED ON CHEMICAL MECHANISMS

基于化学机制的合理药物设计

• 批准号: 2087690
• 负责人: JAMES K COWARD
• 金额: $ 20.12万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-01-01 至 1996-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2087690
• 关键词: antineoplastics; catecholamines; cell free system; cell growth regulation; chemical structure function; chemical substitution; drug adverse effect; drug design /synthesis /production; drug metabolism; enzyme inhibitors; enzyme mechanism; folate; folate antagonist; glutamate ammonia ligase; glutamates; glutamyltransferase; hydrolysis; macromolecule; methotrexate; methotrexate analog; methyltransferase; neoplasm /cancer chemotherapy; neuroblastoma; polyglutamates; tissue /cell culture

During the period of support requested in this application, we plan to continue our efforts on the synthesis of potent and specific enzyme inhibitors based on enzyme mechanism data obtained in our laboratory and from the literature. The proposed studies involve a combination of synthetic organic chemistry, mechanistic enzymology, and biochemical pharmacology. The overall goal of our research is to investigate the role of specific biochemical pathways in the control of cell growth. More specifically, in the first portion of the research proposed herein, we will continue our use of fluoroglutamate-containing folates and antifols to assess the role of polyglutamate biosynthesis and hydrolysis in mammalian cells. For example, we will complete the synthesis of gamma-fluoroleucovorin and use it to define the role of polyglutamates in leucovorin "rescue" of cells following high-dose methotrexate chemotherapy. We will also exploit our recent finding that 3,3-difluoroglutamate (F2Glu) stimulates the formation of polyglutamates in cell-free systems. Following improvement of the synthetic route to F2Glu, we will synthesize a series of F2Glu-containing folates and antifols in order to investigate the biochemical pharmacology of compounds with enhanced ability to form polyglutamate derivatives. In the second portion of the proposed research we will employ our new synthetic methodology for the synthesis of complex acetylenic nucleosides. We will complete the synthesis of our proposed specific "multisubstrate adduct inhibitors" of methylases with which we have many years of experience (catechol O-methyltransferase, phenethanolamine N-methyltransferase) and study their effect on isolated enzymes. If these proposed new methylase inhibitors are as specific and potent as predicted, we will use them to study catecholamine biosynthesis in neuroblastoma cells. As with the folate work, our ultimate goal in this work is to develop potent and specific inhibitors of each methyltransferase in order to study their role in cell growth and/or cell function.

在本申请所要求的支持期间， 我们计划继续努力， 基于酶机制数据的特异性酶抑制剂 在我们的实验室和文献中获得。 的 拟议的研究涉及合成有机化合物， 化学、机械酶学和生化药理学。 我们研究的总体目标是调查 控制细胞生长的特定生化途径。 更具体地说，在研究的第一部分， 在此，我们将继续使用含氟代谷氨酸盐 叶酸和抗叶酸剂来评估聚谷氨酸的作用 在哺乳动物细胞中的生物合成和水解。 比如我们 将完成γ-氟亚叶酸的合成， 为了确定聚谷氨酸在亚叶酸“拯救” 高剂量甲氨蝶呤化疗后的细胞。 我们将 我还利用了我们最近的发现， （F2 Glu）刺激无细胞环境中聚谷氨酸的形成。 系统. 在改进F2 Glu的合成路线后， 我们将合成一系列含F2 Glu的叶酸， antifols，以研究的生化药理学 具有增强的形成聚谷氨酸盐能力的化合物 衍生物. 在研究的第二部分，我们将 采用我们新的合成方法合成复合物 炔属核苷 我们将完成我们的 建议的特定“多底物加合物抑制剂”， 甲基化酶，我们有多年的经验（儿茶酚 0-甲基转移酶、苯乙醇胺N-甲基转移酶）和 研究它们对分离酶的影响。 如果这些新提议 甲基化酶抑制剂的特异性和有效性与预测一样，我们 将用它们来研究 神经母细胞瘤细胞 与叶酸的研究一样，我们的最终目标 这项工作的目的是开发每种药物的有效且特异性的抑制剂 甲基转移酶，以研究它们在细胞生长中的作用 和/或细胞功能。