CHROMOSOME TRANSLOCATED ONCOGENES AND NEOPLASIA
染色体易位癌基因和肿瘤
基本信息
- 批准号:2089073
- 负责人:
- 金额:$ 29.16万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1984
- 资助国家:美国
- 起止时间:1984-07-01 至 1995-05-31
- 项目状态:已结题
- 来源:
- 关键词:B lymphocyte DNA binding protein Retroviridae cell type chromosome disorders chromosome translocation developmental genetics gene expression genetic manipulation genetic mapping genetic transcription laboratory mouse leukocyte activation /transformation molecular cloning molecular oncology neoplasm /cancer genetics neoplastic transformation nucleic acid sequence oncogenes posttranscriptional RNA processing protooncogene transcription factor
项目摘要
The murine and human cellular analogues of an avian retroviral transforming
gene (c-myc) have been directly associated with chromosome translocations
commonly observed in murine plasmacytomas (t(12;15)) and Burkitt lymphomas
(t(8;14)). We propose to continue and extend our studies of the molecular
basis and consequences of myc gene activation for cell transformation.
Different molecular targets for myc rearrangements in murine plasmacytomas
(PCTs) will be localized and their structures and significance for myc
expression determined. To this end, we have recently identified a novel
class of PCTs with translocation breakpoints clustered 5' of c-myc which
alter the regulation of myc expression. The site of these translocations
define the locations of putative regulatory elements important for normal
c-myc gene control. The activities of c-myc promoter and potential
enhancer elements will be assessed by their ability to drive the expression
of a fused gene (i.e., chloramphenicol acetyl transferase or CAT) in
fibroblasts and different lymphoid cell types. The chromatin structures of
transcriptionally active and silent c-myc alleles will be compared to
further characterize important regulatory regions. Modified myc genes will
be stably integrated into lymphoid tumor cells which express either normal
or truncated myc RNAs. The resultant effect(s) on normal and cryptic myc
promoter activities will be determined. The activity of transfected and
endogenous myc genes will also be compared in these transfected cells with
myc exon and intron specific probes. These experiments will allow us to
investigate potential cis or trans systems for regulating myc expression.
The contributions of transcriptional and post-transcriptional mechanisms
for c-myc expression will be assessed in a variety of lymphoid tumor lines
bearing translocations which disrupt the c-myc locus. Finally, murine
retroviral vectors containing either activated myc or human ras oncogenes
will be introduced into normal proliferating B cells and their effects on
growth factor dependency analyzed. (X)
禽逆转录病毒转化的小鼠和人类细胞类似物
项目成果
期刊论文数量(26)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(2)
Chromosome translocations clustered 5' of the murine c-myc gene qualitatively affect promoter usage: implications for the site of normal c-myc regulation.
小鼠 c-myc 基因 5 处聚集的染色体易位定性地影响启动子的使用:对正常 c-myc 调节位点的影响。
- DOI:10.1002/j.1460-2075.1985.tb03800.x
- 发表时间:1985
- 期刊:
- 影响因子:0
- 作者:Yang,JQ;Bauer,SR;Mushinski,JF;Marcu,KB
- 通讯作者:Marcu,KB
PCS, a gene related to the immunoglobulin super family of axonal glycoproteins is expressed in murine plasma cell tumors.
PCS 是一种与轴突糖蛋白免疫球蛋白超家族相关的基因,在小鼠浆细胞肿瘤中表达。
- DOI:10.1007/978-3-642-77633-5_28
- 发表时间:1992
- 期刊:
- 影响因子:0
- 作者:Connelly,MA;Grady,RC;Mushinski,JF;Marcu,KB
- 通讯作者:Marcu,KB
Mode of c-myc gene regulation in folic acid-induced kidney regeneration.
叶酸诱导肾再生中c-myc基因调控的模式。
- DOI:
- 发表时间:1989
- 期刊:
- 影响因子:0
- 作者:Asselin,C;Marcu,KB
- 通讯作者:Marcu,KB
A cis-acting element in the promoter region of the murine c-myc gene is necessary for transcriptional block.
鼠 c-myc 基因启动子区域的顺式作用元件对于转录阻断是必需的。
- DOI:10.1128/mcb.9.12.5340-5349.1989
- 发表时间:1989
- 期刊:
- 影响因子:5.3
- 作者:Miller,H;Asselin,C;Dufort,D;Yang,JQ;Gupta,K;Marcu,KB;Nepveu,A
- 通讯作者:Nepveu,A
Rapid induction of IgM-secreting murine plasmacytomas by pristane and an immunoglobulin heavy-chain promoter/enhancer-driven c-myc/v-Ha-ras retrovirus.
通过降植烷和免疫球蛋白重链启动子/增强子驱动的 c-myc/v-Ha-ras 逆转录病毒快速诱导分泌 IgM 的小鼠浆细胞瘤。
- DOI:10.1073/pnas.85.16.6067
- 发表时间:1988
- 期刊:
- 影响因子:11.1
- 作者:Clynes,R;Wax,J;Stanton,LW;Smith-Gill,S;Potter,M;Marcu,KB
- 通讯作者:Marcu,KB
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KENNETH B MARCU其他文献
KENNETH B MARCU的其他文献
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{{ truncateString('KENNETH B MARCU', 18)}}的其他基金
Novel roles of IKK complex to program gene expression
IKK 复合物在基因表达编程中的新作用
- 批准号:
6682474 - 财政年份:2003
- 资助金额:
$ 29.16万 - 项目类别:
Novel roles of IKK complex to program gene expression
IKK 复合物在基因表达编程中的新作用
- 批准号:
6940725 - 财政年份:2003
- 资助金额:
$ 29.16万 - 项目类别:
Novel roles of IKK complex to program gene expression
IKK 复合物在基因表达编程中的新作用
- 批准号:
6796887 - 财政年份:2003
- 资助金额:
$ 29.16万 - 项目类别:
Novel roles of IKK complex to program gene expression
IKK 复合物在基因表达编程中的新作用
- 批准号:
7115821 - 财政年份:2003
- 资助金额:
$ 29.16万 - 项目类别:
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