CELL TRANSFORMATION BY INSULIN AND IGFI RECEPTORS

这项研究的目的是利用胰岛素受体（IR）和 含胰岛素样生长因子I受体（IGFR）cDNA 逆转录病毒来阐明细胞转化的生化基础。 IR和IGFR是PTK受体家族的两个成员，它们在PTK受体家族中发挥重要作用。 在细胞代谢活动和有丝分裂中起重要调节作用。 我们已经证明， IR和IGFR可以通过将它们的部分cDNA序列置于 逆转录病毒 我们在这项研究中的具体目标是1）鉴定结构 负责激活转化和致瘤的基础 人IR和IGFR的潜力; 2）哺乳动物细胞系的建立 通过含有IR和IGFR的逆转录病毒突变体IR和IGFR转化 蛋白质及其与差异转化电位的关系; 和4）鉴定对以下重要的潜在细胞底物： 通过突变型IR和IGFR蛋白进行细胞转化。 希望本研究能进一步加深我们对 这些受体PTK基因的细胞转化机制。

The goals of this proposed study is to employ insulin receptor (IR) and insulin-like growth factor I receptor (IGFR) CDNA-containing retroviruses to elucidate the biochemical basis of cell transformation. IR and IGFR are two members of the receptor PTK family which play an important regulatory role in cellular metabolic activities and mitosis. We have demonstrated that the transforming and tumorigenic potential of IR and IGFR can be manifested by placing part of their CDNA sequences in retroviruses. Our specific aims in this study are 1) identification of the structural basis responsible for activation of the transforming and tumorigenic potential of human IR and IGFR; 2) establishment of mammalian cell lines transformed by IR and IGFR-containing retroviruses mutant IR and IGFR proteins and correlation with their differential transforming potential; and 4) identification of potential cellular substrates important for cell transformation by mutant IR and IGFR proteins. It is hoped that this study will further our understanding of the mechanism of cell transformation by those receptor PTK genes.