CELL TRANSFORMATION BY INSULIN AND IGFI RECEPTORS

胰岛素和 IGFI 受体的细胞转化

• 批准号: 2096288
• 负责人: LU-HAI WANG
• 金额: $ 18.41万
• 依托单位: MOUNT SINAI SCHOOL OF MEDICINE OF CUNY
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-06-01 至 1997-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2096288
• 关键词: 3T3 cells; Retroviridae; cell transformation; chickens; complementary DNA; genetic mapping; growth factor receptors; insulin; insulin receptor; insulinlike growth factor; laboratory rabbit; monoclonal antibody; nucleic acid sequence; oncogenes; polymerase chain reaction; protein biosynthesis; protein tyrosine kinase; temperature sensitive mutant; western blottings

The goals of this proposed study is to employ insulin receptor (IR) and insulin-like growth factor I receptor (IGFR) CDNA-containing retroviruses to elucidate the biochemical basis of cell transformation. IR and IGFR are two members of the receptor PTK family which play an important regulatory role in cellular metabolic activities and mitosis. We have demonstrated that the transforming and tumorigenic potential of IR and IGFR can be manifested by placing part of their CDNA sequences in retroviruses. Our specific aims in this study are 1) identification of the structural basis responsible for activation of the transforming and tumorigenic potential of human IR and IGFR; 2) establishment of mammalian cell lines transformed by IR and IGFR-containing retroviruses mutant IR and IGFR proteins and correlation with their differential transforming potential; and 4) identification of potential cellular substrates important for cell transformation by mutant IR and IGFR proteins. It is hoped that this study will further our understanding of the mechanism of cell transformation by those receptor PTK genes.

这项拟议研究的目标是利用胰岛素受体（IR）和 含有胰岛素样生长因子 I 受体 (IGFR) cDNA 逆转录病毒阐明细胞转化的生化基础。 IR 和 IGFR 是受体 PTK 家族的两个成员，发挥着重要作用 在细胞代谢活动和有丝分裂中发挥重要的调节作用。 我们已经证明了其转化和致瘤的潜力 IR 和 IGFR 可以通过将其部分 cDNA 序列放入 逆转录病毒。 我们本研究的具体目标是 1) 结构的识别 负责转化和致瘤激活的基础 人类 IR 和 IGFR 的潜力； 2) 哺乳动物细胞系的建立 由含有 IR 和 IGFR 的逆转录病毒突变体 IR 和 IGFR 转化 蛋白质及其差异转化潜力的相关性； 4) 鉴定重要的潜在细胞底物 通过突变 IR 和 IGFR 蛋白进行细胞转化。 希望这项研究能够加深我们对 这些受体PTK基因的细胞转化机制。