GROWTH REGULATION IN CERVICAL NEOPLASIA

描述：(调查员摘要)：在本提案中 调查人员将重点关注以下目标：1)建立 人宫颈HPV阳性细胞系的转染法 以及通过活组织的直接培养；2)研究 这些细胞系对生长因子的反应，特别是转化生长因子-α和 转化生长因子-β，采用常规单层培养和胶原筏系统； 和3)共定位转化生长因子-β和人乳头瘤病毒在组织活检中的表达。 处于异型增生不同阶段的妇女，包括侵袭性癌症。 系统将允许调查人员测试转化生长因子-a和转化生长因子-b的影响 在更可靠的条件下对含乳头瘤病毒的细胞的研究 反映人类的感染情况。原位杂交实验将 揭示转化生长因子-β是否在异常宫颈组织中表达 转化生长因子-β的表达模式及其与细胞因子的关系 表达人乳头瘤病毒的细胞。

DESCRIPTION: (Investigator's Abstract): In this proposal the investigators will focus on the following objectives: 1) to establish HPV-positive cell lines from human cervix by transfection methodologies as well as by direct culture of biopsies; 2) to investigate the regulation of the response of these cell lines to growth factors, especially TGF-a and TGF-b, using conventional monolayer culture and the collagen raft system; and 3) to colocalize TGF-b and HPV mRNA in tissue biopsies obtained from women at different stages of dysplasia including invasive cancer.The raft system will allow the investigators to test the effects of TGF-a and TGF-b on papillomavirus-containing cells under conditions which more faithfully reflect the human infection. In situ hybridization experiments will reveal whether TGF-b is expressed in abnormal cervical tissues, the patterns of expression and the relationship between TGF-b and HPV-expressing cells.