GROWTH REGULATION IN CERVICAL NEOPLASIA

宫颈肿瘤的生长调节

• 批准号: 3200459
• 负责人: LUNDY A BRAUN
• 金额: $ 17.61万
• 依托单位: BROWN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-04-01 至 1995-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3200459
• 关键词: Animalia; biopsy; cell growth regulation; cell line; cell transformation; cervix neoplasms; female; herpes simplex virus 2; human papillomavirus; human subject; in situ hybridization; keratinocyte; messenger RNA; neoplastic growth; oncogenes; posttranscriptional RNA processing; tissue /cell culture; transfection; transforming growth factors

DESCRIPTION: (Investigator's Abstract): In this proposal the investigators will focus on the following objectives: 1) to establish HPV-positive cell lines from human cervix by transfection methodologies as well as by direct culture of biopsies; 2) to investigate the regulation of the response of these cell lines to growth factors, especially TGF-a and TGF-b, using conventional monolayer culture and the collagen raft system; and 3) to colocalize TGF-b and HPV mRNA in tissue biopsies obtained from women at different stages of dysplasia including invasive cancer.The raft system will allow the investigators to test the effects of TGF-a and TGF-b on papillomavirus-containing cells under conditions which more faithfully reflect the human infection. In situ hybridization experiments will reveal whether TGF-b is expressed in abnormal cervical tissues, the patterns of expression and the relationship between TGF-b and HPV-expressing cells.

描述：（研究者摘要）：在本提案中， 研究人员将重点关注以下目标：1）建立 通过转染方法从人宫颈获得HPV阳性细胞系， 以及通过活检组织的直接培养; 2）研究 这些细胞系对生长因子，特别是TGF-α和 TGF-β，采用传统单层培养和胶原筏系统; 和3）将TGF-β和HPV mRNA共定位于从组织活检获得的组织中， 不同阶段的发育不良妇女，包括浸润性癌症。 该系统将允许研究人员测试TGF-a和TGF-b的作用 对含乳头瘤病毒的细胞的作用， 反映了人类的感染。 原位杂交实验将 揭示TGF-b是否在异常宫颈组织中表达， 表达模式以及TGF-β与 HPV表达细胞。