CLONING GENES WITH AN EPISOMAL VECTOR

描述：(改编自《调查者摘要》)本研究提案 涉及到一种可复制的哺乳动物载体系统的发展 同源异构体及其插入新方法的研究进展 将库添加到其中。因为载体仍然是上体，所以它可以是 恢复得又快又快。在这方面，它与以前有所不同 描述了快速整合到宿主(人类)细胞基因组中的载体， 因此，不能轻易恢复。该系统可用于 在基因未知的情况下补充人类细胞的缺陷功能 在那里可以传授选择。提案的一部分是继续 矢量系统的开发。此外，调查人员还提出 识别以前未被识别的人类基因：1) 共济失调毛细血管扩张，特异性互补D组；2)基因(S) 编码甲氨蝶呤转运的基因，这些基因的形式有缺陷 在癌细胞中的一种常见形式的甲氨蝶呤耐药性。

DESCRIPTION: (Adapted from Investigator's Abstract) This research proposal involves the development of a mammalian vector system that replicates episomally and the development of new methods for the insertion of cDNA libraries into it. Because the vector remains episomal, it can be recovered rapidly and readily. In this respect it differs from previously described vectors that integrate rapidly into the host (human) cell genome, and hence, cannot be recovered readily. This system can be used to complement defective functions of human cells where the gene is unknown and where a selection can be imparted. Part of the proposal is to continue development of the vector system. Additionally the investigators propose to identify human genes which have not previously been identified: 1) Ataxia telangiectasia, specifically complementation group D; 2) gene(s) coding for methotrexate transport, genes which in a defective form result in a common form of methotrexate resistance in cancer cells.