ENZYME SYNTHESIS AND DEGRADATION IN METABOLIC CONTROL
ENZYME SYNTHESIS AND DEGRADATION IN METABOLIC CONTROL
批准号:
3268701
负责人:
ROBERT T SCHIMKE
金额:
$19.43万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1976
资助国家:
美国
项目状态:
已结题
起止时间:
1976-09-01 至 1986-11-30
中文摘要
我们将研究培养的动物细胞变成
英文摘要
We shall be studying the mechanism whereby cultured animal cells become
resistant to methotrexate as a result of selective amplification of DNA
sequences coding for dihydrofolate reductase. The research is directed at
the fundamental mechanism(s) whereby this occurs and results in amplified
DNA sequences that are either present on chromosomes, or are present at
extrachomosomal elements called "double minute chromosomes." Gene
amplification occurs on evolutionary time scale, as well as one mechanism
for developmental regulatino of protein synthesis. We wish to use the
experimental system of cultured mammalian cells to study the mechanism of
amplification. The studies will employ varoius cells lines of cultured
hamseter and mouse cells. Mechanisms to be studied include
uptake-replicaton-integration of DNA from cells, as well as a saltatory
replication model. Techniques to be employed include use of
fluorescence-activated cell sorter to study DNA synthesis, as well as
isolate cells with elevated dihydrofolate reductase levels. Dihydrofolate
reductase DNA sequences will be detected and isolate employing both
messenger-derived, as well as genomic recombinant DNA clones presently
available in the laboratory. Ultimately, we are interested in studing how
genes are amplified, and what intrinsic and extrinsic factors may affect
the amplification process.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
CLONING GENES WITH AN EPISOMAL VECTOR
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批准号:3198870
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项目类别:
-
资助金额:$24.06万
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财政年份:1991
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负责人:ROBERT T SCHIMKE
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依托单位:
CLONING GENES WITH AN EPISOMAL VECTOR
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批准号:3198868
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项目类别:
-
资助金额:$21.57万
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财政年份:1991
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负责人:ROBERT T SCHIMKE
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依托单位:
CLONING GENES WITH AN EPISOMAL VECTOR
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批准号:3198869
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项目类别:
-
资助金额:$22.44万
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财政年份:1991
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负责人:ROBERT T SCHIMKE
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依托单位:
GENOME EVOLUTION/REGULATION OF PROTEIN LEVELS IN AGING
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批准号:3091022
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项目类别:
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资助金额:$61.8万
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财政年份:1985
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负责人:ROBERT T SCHIMKE
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依托单位:
GENOME EVOLUTION/REGULATION OF PROTEIN LEVELS IN AGING
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批准号:3091019
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项目类别:
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资助金额:$58.69万
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财政年份:1985
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负责人:ROBERT T SCHIMKE
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依托单位:
GENOME EVOLUTION/REGULATION OF PROTEIN LEVELS IN AGING
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批准号:3091024
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项目类别:
-
资助金额:$56.5万
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财政年份:1985
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负责人:ROBERT T SCHIMKE
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依托单位:
GENOME EVOLUTION/REGULATION OF PROTEIN LEVELS IN AGING
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批准号:3091023
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项目类别:
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资助金额:$59.11万
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财政年份:1985
-
负责人:ROBERT T SCHIMKE
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依托单位:
GENOME EVOLUTION/REGULATION OF PROTEIN LEVELS IN AGING
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批准号:3091021
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项目类别:
-
资助金额:$74.58万
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财政年份:1985
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负责人:ROBERT T SCHIMKE
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依托单位:
MOLECULAR MECHANISM IN RESISTANCE TO FOLATE ANALOGUES
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批准号:3164383
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项目类别:
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资助金额:$33.01万
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财政年份:1977
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负责人:ROBERT T SCHIMKE
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依托单位:
MOLECULAR MECHANISM IN RESISTANCE TO FOLATE ANALOGUES
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批准号:3164386
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项目类别:
-
资助金额:$31.99万
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财政年份:1977
-
负责人:ROBERT T SCHIMKE
-
依托单位:
MOLECULAR MECHANISM IN RESISTANCE TO FOLATE ANALOGUES
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批准号:3164385
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项目类别:
-
资助金额:$32.26万
-
财政年份:1977
-
负责人:ROBERT T SCHIMKE
-
依托单位:
MOLECULAR MECHANISM IN RESISTANCE TO FOLATE ANALOGUES
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批准号:3164382
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项目类别:
-
资助金额:$24.09万
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财政年份:1977
-
负责人:ROBERT T SCHIMKE
-
依托单位:
MOLECULAR MECHANISM IN RESISTANCE TO FOLATE ANALOGUES
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批准号:3164384
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项目类别:
-
资助金额:$29.74万
-
财政年份:1977
-
负责人:ROBERT T SCHIMKE
-
依托单位:
MOLECULAR MECHANISM IN RESISTANCE TO FOLATE ANALOGUES
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批准号:3164387
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项目类别:
-
资助金额:$24.95万
-
财政年份:1977
-
负责人:ROBERT T SCHIMKE
-
依托单位:
MOLECULAR MECHANISM IN RESISTANCE TO FOLATE ANALOGUES
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批准号:3164388
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项目类别:
-
资助金额:$25.13万
-
财政年份:1977
-
负责人:ROBERT T SCHIMKE
-
依托单位:
MOLECULAR MECHANISM IN RESISTANCE TO FOLATE ANALOGUES
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批准号:3164381
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项目类别:
-
资助金额:$33.5万
-
财政年份:1977
-
负责人:ROBERT T SCHIMKE
-
依托单位:
MOLECULAR MECHANISM IN RESISTANCE TO FOLATE ANALOGUES
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批准号:3164389
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项目类别:
-
资助金额:$27.88万
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财政年份:1977
-
负责人:ROBERT T SCHIMKE
-
依托单位:
MOLECULAR MECHANISM IN RESISTANCE TO FOLATE ANALOGUES
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批准号:2086463
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项目类别:
-
资助金额:$28.55万
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财政年份:1977
-
负责人:ROBERT T SCHIMKE
-
依托单位:
ENZYME SYNTHESIS AND DEGRADATION IN METABOLIC CONTROL
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批准号:3268697
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项目类别:
-
资助金额:$30.86万
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财政年份:1976
-
负责人:ROBERT T SCHIMKE
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依托单位:
ENZYME SYNTHESIS AND DEGRADATION IN METABOLIC CONTROL
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批准号:3268700
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项目类别:
-
资助金额:$1.62万
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财政年份:1976
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负责人:ROBERT T SCHIMKE
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依托单位:
海外基金