CHARACTERIZATION OF GP35--A SIGNAL TRANSDUCER ON NK CELL

描述：（改编自申请人摘要）拟定研究 gp 35是一种33-40 kD的糖蛋白， 与配体结合结构相互作用的信号转导分子 以调节NK细胞和T细胞的细胞反应。 的 具体的目的是：1）确定gp 35单抗是否是促有丝分裂的， 新鲜分离的NK细胞，影响NK细胞的细胞溶解活性，和 激活NK细胞产生细胞因子; 2）确定是否有 与NK上gp 35物理相关的细胞表面分子 细胞和T细胞; 3）通过产生gp 35缺失突变体， RNK-16，gp 35是否是靶细胞诱导的PPI周转所必需的 在RNK-16内，用于细胞毒性和用于CD 2介导的信号传导;和4） 克隆编码gp 35的基因。

DESCRIPTION: (Adapted from the applicant's abstract) The proposed studies will address the hypothesis that gp35 a 33-40 kD glycoprotein is a signal-transducing molecule that interacts with ligand-binding structures in order to regulate the cellular responses of NK cells and T cells. The specific aims are: 1) to determine whether gp35 MAb are mitogenic for freshly isolated NK cells, affect the cytolytic activity of NK cells, and activate NK cells to produce cytokines; 2) to determine whether there are cell-surface molecules which are physically associated with gp35 on NK cells and T cells; 3) to determine, by generating gp35-loss mutants of RNK-16, whether gp35 is required for target cell-induced PPI turnover within RNK-16, for cytotoxicity, and for CD2-mediated signaling; and 4) to clone the gene that encodes gp35.