DNA HELICASES AND REPLICATION OF BOVINE PAPILLOMA VIRUS

复制双链DNA需要形成单链 DNA以支持进入核苷酸的Watson-Crick碱基配对 在合成子链的过程中。DNA解旋酶是一种酶 导致双链DNA解链，并在复制时发现 叉子。这项建议的长期目标是确定和 描述在DNA复制中起重要作用的DNA解旋酶。 该提案包括鉴定、隔离和表征 依赖于人类DNA结合蛋白的DNA解旋酶 它们的活性，是人类DNA结合蛋白中唯一的三个亚单位 支持含SV40的DNA复制的结合蛋白 起源。这种结合蛋白也被证明是 修复烷化或紫外线损伤的DNA。 小鼠无细胞体外复制牛乳头瘤病毒DNA 提取物依赖于病毒编码的蛋白质EL和E2。《EL》 蛋白质已被证明含有DNA解旋酶活性，并与 复制的最小BPV来源。我们计划研究这一机制。 通过它来调控DNA的复制。我们将研究一下 依赖于BPV核心的解卷反应的细胞周期控制 由EL蛋白催化的起源。 DNA复制的调节在正常情况下是相当重要的 细胞。从这里描述的研究中获得的信息可以 有助于我们了解癌细胞如何逃脱这种控制。

The replication of duplex DNA requires the formation of single stranded DNA in order to support Watson-Crick base pairing of entering nucleotides in the synthesis of daughter strands. DNA helicases are enzymes which lead to the unwinding of duplex DNA and are found at the replication fork. The long-term objective of this proposal is to identify and characterize DNA helicases that are important in the replication of DNA. The proposal includes the identification, isolation and characterization of DNA helicases that are dependent on the human DNA binding protein for their activity, The three subunit human DNA binding protein is the only binding protein which supports the replication of DNA containing the SV40 origin. This binding protein was also shown to be required for the repair of alkylated or ultraviolet damaged DNA. The replication of Bovine Papilloma virus DNA in vitro by mouse cell free extracts depends upon the viral encoded proteins El and E2. The El protein has been shown to contain DNA helicase activity and binds to the minimal BPV origin of replication. We plan to investigate the mechanism by which the replication of this DNA is regulated. We shall examine the cell cycle control of the unwinding reaction dependent on the BPV core origin catalyzed by the El protein. The regulation of DNA replication is of considerable importance in normal cells. The information derived from the studies described here may contribute to our knowledge of how cancer cells escape this control.