RADIATION INDUCED MUTATION SPECTRA AT THE HPRT LOCUS

HPRT 位点的辐射诱发突变谱

• 批准号: 2097293
• 负责人: DAVID J CHEN
• 金额: $ 21.94万
• 依托单位: UNIVERSITY OF CALIF-LOS ALAMOS NAT LAB
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-06-01 至 1996-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2097293
• 关键词: alleles; alpha radiation; artificial chromosomes; clone cells; fibroblasts; frameshift mutation; gamma radiation; gene deletion mutation; gene frequency; genetic markers; human genetic material tag; human tissue; hypoxanthine phosphoribosyltransferase; ionizing radiation; linear energy transfer; nucleic acid sequence; point mutation; polymerase chain reaction; pulsed field gel electrophoresis

The objective of this proposed research is to determine whether characteristic mutation spectra (types and frequencies) can be induced at or proximal to the HPRT locus in normal human fibroblasts exposed to various forms of ionizing radiation. If unique mutational spectra can be demonstrated, then this can serve as a molecular signature to differentiate low and high LET radiation induced genetic damage from that which occur spontaneously. The specific aims are: 1) to determine whether the spectra of point mutations induced by gamma-rays and alpha-particles are significantly different from that which occur spontaneously; and 2) to determine whether the size distribution of deletions induced by various forms of radiation is dependent on the quality of radiation and can be differentiated from that which occurs spontaneously.

这项研究的目的是确定是否 特征突变谱（类型和频率）可以在 或接近于暴露于以下物质的正常人成纤维细胞中的HPRT基因座 各种形式的电离辐射 如果独特的突变谱可以 证明，那么这可以作为一个分子签名，以区分 低和高LET辐射诱导的遗传损伤， 自发的 具体目的是：1）确定光谱是否 由伽马射线和α粒子引起的点突变 与自发发生的显著不同;以及2） 确定是否由各种诱导的缺失的大小分布 辐射的形式取决于辐射的质量， 区别于自发发生的。