QUANTITATION OF ALLELIC IMBALANCE IN SOLID TUMORS

等位基因缺失或杂合性缺失(LOH)，是一种遗传 肿瘤细胞中的异常，是肿瘤抑制基因突变和 1或人类癌症发生或发展过程中的基因组不稳定性。 某些遗传区域的等位基因丢失，以及全基因组的索引 等位基因缺失，已被证明是远处转移的良好预测因子 和结直肠癌的存活率，但临床应用受阻于 传统LOH方法的几个技术缺陷。整体而言 本研究的目的是改进等位基因丢失的测量方法。 并演示这种测量的实际用途。通过使用 基于聚合酶链式反应的微卫星重复序列多态性与定量荧光粉 影像分析，我们将探索检测等位基因的可行性 临床档案(福尔马林固定、石蜡包埋)的不平衡 前列腺癌标本，以及此类标本的自动化和放大 允许快速测量全基因组等位基因丢失的程序。这些 研究可能在癌症研究和临床中带来新的应用 医药。

Allelic loss or "loss of heterozygosity" (LOH), a type of genetic abnormality in tumor cells, is a marker of tumor suppressor mutation and 1 or genomic instability in the genesis or progression of human cancers. Allelic losses of certain genetic regions, and an index of whole-genome allelic loss, have been shown to be good predictors of distant metastasis and survival in colorectal cancer, but clinical application is hindered by several technical drawbacks of traditional LOH methods. The overall objective of this study is to improve methods for measuring allelic loss and to demonstrate practical uses for such measurement. With the use of PcR-based microsatellite repeat polymorphisms and quantitative phosphor imaging analysis, we will investigate the feasibility of detecting allelic imbalances in archival (formalin-fixed, paraffin-embedded) clinical prostate cancer specimens, and of automation and scale-up of such procedures to allow rapid measurement of genome-wide allelic loss. These studies could lead to new applications in cancer research and clinical medicine.