MEASUREMENT OF DNA ADDUCTS IN HUMAN SAMPLES

描述(改编自申请者摘要)：调查人员 我开发了一种测量N7-鸟氨酸(N7-G)DNA加合物的方法 环氧乙烷和苯乙烯。由于环氧乙烷和苯乙烯 在工业上广泛使用，在动物身上会致癌， 调查人员建议将这种方法应用于工人的血液样本 在职业上接触过这些特工。如果这样的DNA加合物可以 然后，该项目可能会导致更好地理解在哪里设置 从事这些化学品工作的人的职业暴露限值。 在他们声称既方便又强大的方法中， N7-G加合物很容易从DNA中获得，只需将DNA加热到 水。然后使用简单的试剂和步骤来衍生化和纯化 用质谱仪检测前的加合物。同样的方法 在未来可用于检测其他简单烷基和 工业或环境化学品形成的羟基烷基N7-G加合物 不包括环氧乙烷或苯乙烯。

DESCRIPTION (Adapted from the Applicant's Abstract): The investigators have developed a method for measuring the N7-guanine (N7-G) DNA adducts of ethylene oxide and styrene. Since ethylene oxide and styrene are widely used in industry and are tumorigenic in animals, the investigators propose to apply the method to blood samples of workers exposed to these agents occupationally. If such DNA adducts can be then the project may lead to a better understanding of where to set occupational exposure limits for those working with these chemicals. In their method, which they claim is both convenient and powerful, the N7-G adducts are easily harvested from DNA by simply heating the DNA in water. Simple reagents and steps are then used to derivatize and purify the adducts prior to detection by mass spectrometry. The same method can in the future find use in the detection of other simple alkyl and hydroxalkyl N7-G adducts formed by industrial or environmental chemicals other than ethylene oxide or styrene.