CONFORMATIONAL TRANSITIONS IN OLIGOPURINE TRACTS

下面的FIRCA应用程序描述了一个建议的协作工作 在化学系Michael J. Behe博士的实验室之间， 利哈伊大学和生物物理研究所的Jaroslav Kypr博士， 捷克斯洛伐克科学院 在合作努力中， Behe博士的实验室将合成并发送到Kypr博士的实验室 Behe博士的实验室正在研究一系列合成的多聚脱氧核苷酸 目前正在进行的与父母补助金资助的工作有关的工作。 Kypr博士的实验室随后将检查多核苷酸， 转换为A，X和三链体DNA构象作为一个函数， 多核苷酸的重复单元。 这些实验需要 Kypr博士实验室在以下方面的丰富经验的优势： 使用圆二色性（CD）的DNA构象异构化 光谱和其他方法。 与CD同时，Kypr博士 实验室将用紫外线照射多核苷酸， 通过变性凝胶电泳检测照射的多核苷酸。 等 实验的动机是初步结果表明， 链间交联，当在X-DNA构象中照射时，但 而不是在B-DNA或A-DNA构象中照射时。

The following FIRCA application describes a proposed collaborative effort between the laboratories of Dr. Michael J. Behe, Department of Chemistry, Lehigh University, and Dr. Jaroslav Kypr, Institute of Biophysics, Czechoslovak Academy of Sciences. In the collaborative effort the laboratory of Dr. Behe will synthesize and send to Dr. Kypr's laboratory a range of synthetic polydeoxynucleotides which Dr. Behe's laboratory is currently making in connection with work funded under the parent grant. The laboratory of Dr. Kypr will then examine the polynucleotides for transitions to the A, X, and triplex DNA conformations as a function of the repeating unit of the polynucleotide. The experiments will take advantage of the extensive experience of Dr. Kypr's lab with studies of DNA conformational isomerizations using circular dichroism (CD) spectroscopy and other methods. In parallel with CD, Dr. Kypr's laboratory will irradiate the polynucleotides with uv light and analyze the irradiated polynucleotides by denaturing gel electrophoresis. Such experiments are motivated by preliminary results showing that the interstrand crosslinking, when irradiated in the X-DNA conformation, but not when irradiated in the B-DNA or A-DNA conformations.