GUSTATORY NST--PATHWAYS, SYNAPSES AND NEUROCHEMICALS
GUSTATORY NST--PATHWAYS, SYNAPSES AND NEUROCHEMICALS
批准号:
2127160
负责人:
MARK Celestin WHITEHEAD
金额:
$13.78万
依托单位国家:
美国
项目类别:
财政年份:
1994
资助国家:
美国
项目状态:
已结题
起止时间:
1994-04-01 至 1998-03-31
关键词:
afferent nerve axon cell type central neural pathway /tract cholera toxin chorda tympani cranial nerves electron microscopy gamma aminobutyrate hamsters histochemistry /cytochemistry immunocytochemistry interneurons light microscopy nerve endings neural information processing neuroanatomy neurochemistry reticular formation solitary tract nucleus substance P synapses taste tyrosine 3 monooxygenase
中文摘要
孤束吻端核细胞类型的研究
(NST)它们的连接和突触关系对于
了解哺乳动物味觉系统的基本结构。
这些研究的一个主要目标是为这些人开发一个写作图表。
味觉NST,其中不同成分之间的连接是
基于它们的形态在突触水平上鉴定,
它们的原始细胞和它们的神经化学物质或神经递质
特色 提供有关突触组织的信息
在吻侧NST的电路是有限的。
拟议的研究将提供有关
NST的形态学和神经化学组织,
关于其神经元结构和突触组织的最新发现
在仓鼠身上 传入轴突之间的突触相互作用,
投射神经元和含γ-氨基丁酸(GABA能)
将通过电子显微镜结合
轴突和细胞标记方法和免疫细胞化学。 的
方法涉及一系列的标签研究,重点是吻侧
NST的中央分区。 此区域接收denial输入
来自味觉初级传入轴突,包含大部分臂旁神经
(PBN)-投射细胞和许多GABA能细胞。 Axon终端将
用辣根过氧化物酶(HRP)或霍乱毒素(Ct)标记,
神经化学成分(例如GABA),或使用顺行性
退化 在某些材料中,将使用第二个对比标签
根据连接(HRP或CT)证明细胞类型,或
化学特性(如GABA)。 这些研究将决定
味觉传入轴突是否与PBN投射细胞突触,
髓内投射细胞或GABA能中间神经元。
来自不同传入轴突的输入的会聚(例如,索
鼓膜和舌咽神经)对所鉴定的细胞类型的影响将被
评估。 下行轴突的精细结构和突触部位
从前脑和轴突特征免疫细胞化学(例如,
GABA)也将被确定。 这些研究的数据应该允许
关于孤核的电路如何
处理和传播味觉信息。 知识
味觉系统的微电路需要更完整的
味觉障碍的理解及其进展。
英文摘要
Studies of the cell types of the rostral nucleus of the solitary tract
(NST), their connections and synaptic relationships, are essential for
understanding the basic organization of the mammalian gustatory system.
A major goal of such studies is to develop a writing diagram for the
gustatory NST in which connections between different components are
identified at the synaptic level on the basis of their morphologies,
their cells of origin and their neurochemical or neurotransmitter
characteristics. Present information about the organization of synaptic
circuits in the rostral NST is limited.
The proposed research will provide detailed information about the
morphological and neurochemical organization of the NST in light of
recent findings on its neuronal architecture and synaptic organization
in the hamster. The synaptic interactions between afferent axons,
projection neurons and Gamma aminobutyric acid-containing (GABAergic)
interneurons will be characterized by electron microscopy combined with
axonal and cellular labelling methods and immunocytochemistry. The
approach involves a series of labelling studies focused on the rostral
central subdivision of the NST. This region receives the densest input
from gustatory primary afferent axons and contains most of parabrachial
(PBN)-projection cells an many GABAergic cells. Axon terminals will be
labelled with horseradish peroxidase (HRP) or cholera toxin (Ct), for
neurochemical content (e.g. GABA), or identified using anterograde
degeneration. In some material a second, contrasting label will be used
to demonstrate cell types on the basis of connections (HRP or CT) or
chemical characteristics (e.g. GABA). These studies will determine
whether taste afferent axons synapse with PBN-projection cells, with
intramedullary projection cells, or with GABAergic interneurons.
Convergence of inputs from different afferent axons (e.g. the chorda
tympani and the glossopharyngeal nerve) on identified cell types will be
evaluated. The fine structure and sites of synapsis of axons descending
from the forebrain and axons characterized immunocytochemically (e.g. for
GABA) will also be determined. Data from these studies should allow the
formulation of hypotheses about how the circuitry of the solitary nucleus
processes and disseminates gustatory information. Knowledge of the
microcircuitry of the gustatory system is required for more complete
understanding of taste disorders and their progress.
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GUSTATORY NST--PATHWAYS, SYNAPSES AND NEUROCHEMICALS
-
批准号:2127162
-
项目类别:
-
资助金额:$14.14万
-
财政年份:1994
-
负责人:MARK Celestin WHITEHEAD
-
依托单位:
GUSTATORY NST--PATHWAYS, SYNAPSES AND NEUROCHEMICALS
-
批准号:2391116
-
项目类别:
-
资助金额:$14.7万
-
财政年份:1994
-
负责人:MARK Celestin WHITEHEAD
-
依托单位:
GUSTATORY NST--PATHWAYS, SYNAPSES AND NEUROCHEMICALS
-
批准号:2127161
-
项目类别:
-
资助金额:$13.58万
-
财政年份:1994
-
负责人:MARK Celestin WHITEHEAD
-
依托单位:
NERVE-TARGET CELL INTERACTIONS IN TASTE BUD MAINTENANCE
-
批准号:2126939
-
项目类别:
-
资助金额:$10.12万
-
财政年份:1993
-
负责人:MARK Celestin WHITEHEAD
-
依托单位:
NERVE-TARGET CELL INTERACTIONS IN TASTE BUD MAINTENANCE
-
批准号:3218471
-
项目类别:
-
资助金额:$10.82万
-
财政年份:1993
-
负责人:MARK Celestin WHITEHEAD
-
依托单位:
NERVE-TARGET CELL INTERACTION IN TASTE BUD MAINTENANCE
-
批准号:6820959
-
项目类别:
-
资助金额:$29.47万
-
财政年份:1993
-
负责人:MARK Celestin WHITEHEAD
-
依托单位:
NERVE-TARGET CELL INTERACTION IN TASTE BUD MAINTENANCE
-
批准号:7240483
-
项目类别:
-
资助金额:$26.23万
-
财政年份:1993
-
负责人:MARK Celestin WHITEHEAD
-
依托单位:
NERVE-TARGET CELL INTERACTIONS IN TASTE BUD MAINTENANCE
-
批准号:2126940
-
项目类别:
-
资助金额:$10.55万
-
财政年份:1993
-
负责人:MARK Celestin WHITEHEAD
-
依托单位:
NERVE-TARGET CELL INTERACTION IN TASTE BUD MAINTENANCE
-
批准号:7068034
-
项目类别:
-
资助金额:$27.01万
-
财政年份:1993
-
负责人:MARK Celestin WHITEHEAD
-
依托单位:
NERVE TARGET CELL INTERACTION IN TASTE BUD MAINTENANCE
-
批准号:6131797
-
项目类别:
-
资助金额:$20.15万
-
财政年份:1993
-
负责人:MARK Celestin WHITEHEAD
-
依托单位:
Cell Types, Circuits, and Plasticity in the Mouse Taste System
-
批准号:8286430
-
项目类别:
-
资助金额:$25.42万
-
财政年份:1993
-
负责人:MARK Celestin WHITEHEAD
-
依托单位:
Cell Types, Circuits, and Plasticity in the Mouse Taste System
-
批准号:8670714
-
项目类别:
-
资助金额:$25.42万
-
财政年份:1993
-
负责人:MARK Celestin WHITEHEAD
-
依托单位:
Cell Types, Circuits, and Plasticity in the Mouse Taste System
-
批准号:7986293
-
项目类别:
-
资助金额:$27.7万
-
财政年份:1993
-
负责人:MARK Celestin WHITEHEAD
-
依托单位:
Cell Types, Circuits, and Plasticity in the Mouse Taste System
-
批准号:8096581
-
项目类别:
-
资助金额:$25.42万
-
财政年份:1993
-
负责人:MARK Celestin WHITEHEAD
-
依托单位:
NERVE-TARGET CELL INTERACTION IN TASTE BUD MAINTENANCE
-
批准号:7442237
-
项目类别:
-
资助金额:$25.89万
-
财政年份:1993
-
负责人:MARK Celestin WHITEHEAD
-
依托单位:
NERVE-TARGET CELL INTERACTION IN TASTE BUD MAINTENANCE
-
批准号:6516116
-
项目类别:
-
资助金额:$15.2万
-
财政年份:1993
-
负责人:MARK Celestin WHITEHEAD
-
依托单位:
NERVE-TARGET CELL INTERACTION IN TASTE BUD MAINTENANCE
-
批准号:6379315
-
项目类别:
-
资助金额:$15.19万
-
财政年份:1993
-
负责人:MARK Celestin WHITEHEAD
-
依托单位:
Cell Types, Circuits, and Plasticity in the Mouse Taste System
-
批准号:8477167
-
项目类别:
-
资助金额:$24.15万
-
财政年份:1993
-
负责人:MARK Celestin WHITEHEAD
-
依托单位:
NERVE-TARGET CELL INTERACTION IN TASTE BUD MAINTENANCE
-
批准号:6833168
-
项目类别:
-
资助金额:$4.0万
-
财政年份:1993
-
负责人:MARK Celestin WHITEHEAD
-
依托单位:
NERVE-TARGET CELL INTERACTION IN TASTE BUD MAINTENANCE
-
批准号:6908082
-
项目类别:
-
资助金额:$27.66万
-
财政年份:1993
-
负责人:MARK Celestin WHITEHEAD
-
依托单位:
海外基金