CORE--LIPID AND LIPOPROTEIN ANALYSIS

核心C的目标是提供脂蛋白组分和 仔细标准化的脂类分析程序和 脱脂蛋白供该计划内的个别项目使用。这 核心设施有四个超速离心机和各种转子用于制备， 分区和平衡超速离心技术，密度梯度 带有吸光度监测器的分馏器，折射计，分光光度计， 一台气相色谱仪，一台微型离心机，3台低速离心机，一台IBM个人 计算机、计算机驱动的西格玛扫描数字化仪和接口的计算机 伊莉莎·里德。然而，正如为这一核心请拨的预算中所述，a 数字多移液器和ELISA板洗涤器，目前还没有 已被要求在实验室中使用。这些设备 将方便处理多个样品和洗涤 载脂蛋白酶联免疫吸附试验容量增加对多个酶联免疫吸附试验的要求 在当前应用程序中预期的。

The objective of Core C is to provide lipoprotein fractions and the carefully standardized procedures for the analyses of lipids and apoproteins for use by the individual projects within the program. This Core facility has four ultracentrifuges and various rotors for preparative, zonal and equilibrium ultracentrifugal techniques, a density gradient fractionator with absorbance monitor, a refractometer, a spectrophotometer, a gas chromatograph, a microfuge, 3 low speed centrifuges, an IBM personal computer, a computer driven Sigma Scan Digitizer, and a computer interfaced ELISA Reader. However, as stated in the budget requested for this Core, a digital multipipettor and an ELISA plate washer, which currently are not available in the laboratory, have been requested. These equipment items will facilitate the handling of multiple samples and the washing of multiple ELISA plates demanded by the increased volume of apoprotein ELISA anticipated in the current application.