CORE--LIPID AND LIPOPROTEIN ANALYSIS

核心——脂质和脂蛋白分析

• 批准号: 3735987
• 负责人: GODFREY S GETZ
• 金额: --
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3735987
• 关键词: apolipoproteins; biomedical facility; blood lipid; blood lipoprotein; cholesterol; enzyme linked immunosorbent assay; high density lipoproteins; immunoaffinity chromatography; immunologic assay /test; triglycerides

The objective of Core C is to provide lipoprotein fractions and the carefully standardized procedures for the analyses of lipids and apoproteins for use by the individual projects within the program. This Core facility has four ultracentrifuges and various rotors for preparative, zonal and equilibrium ultracentrifugal techniques, a density gradient fractionator with absorbance monitor, a refractometer, a spectrophotometer, a gas chromatograph, a microfuge, 3 low speed centrifuges, an IBM personal computer, a computer driven Sigma Scan Digitizer, and a computer interfaced ELISA Reader. However, as stated in the budget requested for this Core, a digital multipipettor and an ELISA plate washer, which currently are not available in the laboratory, have been requested. These equipment items will facilitate the handling of multiple samples and the washing of multiple ELISA plates demanded by the increased volume of apoprotein ELISA anticipated in the current application.

Core C 的目的是提供脂蛋白级分和 精心标准化的脂质分析程序 供程序内各个项目使用的脱辅基蛋白。 这 核心设施有四个超速离心机和各种转子，用于制备、 区带和平衡超离心技术，密度梯度 带吸光度监测器的分馏器、折射计、分光光度计、 气相色谱仪、微量离心机、3 台低速离心机、IBM 个人计算机 计算机、计算机驱动的 Sigma 扫描数字化仪和计算机接口 ELISA 阅读器。 然而，正如该核心要求的预算所述， 数字多管移液器和 ELISA 洗板机，目前还没有 已要求在实验室提供。 这些装备项目 将有利于多个样品的处理和清洗 载脂蛋白 ELISA 体积增加，需要多个 ELISA 板 当前应用中预期的。