CORE--LIPID AND LIPOPROTEIN ANALYSIS

核心——脂质和脂蛋白分析

• 批准号: 5213153
• 负责人: GODFREY S GETZ
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/5213153
• 关键词: apolipoproteins; biomedical facility; blood lipid; blood lipoprotein; cholesterol; enzyme linked immunosorbent assay; high density lipoproteins; immunoaffinity chromatography; immunologic assay /test; triglycerides

The objective of Core C is to provide lipoprotein fractions and the carefully standardized procedures for the analyses of lipids and apoproteins for use by the individual projects within the program. This Core facility has four ultracentrifuges and various rotors for preparative, zonal and equilibrium ultracentrifugal techniques, a density gradient fractionator with absorbance monitor, a refractometer, a spectrophotometer, a gas chromatograph, a microfuge, 3 low speed centrifuges, an IBM personal computer, a computer driven Sigma Scan Digitizer, and a computer interfaced ELISA Reader. However, as stated in the budget requested for this Core, a digital multipipettor and an ELISA plate washer, which currently are not available in the laboratory, have been requested. These equipment items will facilitate the handling of multiple samples and the washing of multiple ELISA plates demanded by the increased volume of apoprotein ELISA anticipated in the current application.

核心C的目的是提供脂蛋白组分， 仔细标准化的程序，用于分析脂质和 载脂蛋白，供程序中的各个项目使用。 这 核心设施有四个超级离心机和各种转子， 分区和平衡超离心技术，密度梯度 具有吸光度监测器分馏器、折射计、分光光度计 一台气相色谱仪，一台微量离心机，三台低速离心机，一台IBM个人 计算机、计算机驱动的Sigma扫描数字化仪和计算机接口 ELISA读数器。 然而，如本核心预算所述， 数字多移液器和ELISA洗板机，目前还没有 在实验室里，他们被要求。 这些设备 将有助于处理多个样品和洗涤 载脂蛋白ELISA体积增加所需的多个ELISA板 在当前应用程序中预期。