NON MUSCLE HEAVY CHAIN GENE

非肌肉重链基因

• 批准号: 2210006
• 负责人: LUCY W ARNOLD
• 金额: $ 7.83万
• 依托单位: BOSTON CHILDREN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-04-01 至 1995-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2210006
• 关键词: cell motility; complementary DNA; electroporation; gel electrophoresis; gene expression; genetic mapping; genetic recombination; genome; laboratory mouse; laboratory rat; microinjections; molecular cloning; myosins; natural gene amplification; nucleic acid hybridization; nucleic acid probes; nucleic acid sequence; polymerase chain reaction; protein biosynthesis; protein sequence; protein structure function; sarcomeres; site directed mutagenesis

Nonmuscle (nm) myosin is an essential part of the cytoskeleton, a complex intracellular network that orchestrates such cell functions as cytokinesis, motility, and muscle contraction. Recent work has demonstrated that dictyostelium lacking myosin heavy chain (mhc) are viable, motile, and phagocytic, although they demonstrate markedly abnormal cytokinesis. In dividing mammalian cells, nm myosin localizes to the cleavage furrow during cytokinesis; labeled myosin light chains microinjected into living cells localize in the cleavage furrow, and become reincorporated into stress fibers at the completion of cytokinesis. Nm myosin may also be necessary for sarcomere assembly, because the cytoskeleton must act as a scaffolding for synthesis of the sarcomeric contractile proteins to occur by cotranslational assembly. Exciting advances in gene targeting now make it possible to target nonselectable genes by homologous recombination. Preliminary data suggests that the nm mhc gene is encoded by one or a few genes making such gene targeting feasible. The primary goal of this project is to complete the cloning and characterization of the nm mhc gene, in order to begin gene disruption experiments by homologous recombination. By disrupting the nm mhc in mouse non and muscle cells, we should be able to elucidate the role of nm mhc in cytokinesis, cell motility and sarcomere assembly. Further studies will focus on identifying those gene domains that confer specific functions to the nm mhc protein in both muscle and nonmuscle.

非肌（nm）肌球蛋白是细胞骨架（一种复合体）的重要组成部分

项目成果

Noninvasive determination of cardiac output in a model of acute lung injury.

急性肺损伤模型中心输出量的无创测定。

• DOI: 10.1097/00003246-199705000-00024
• 发表时间: 1997
• 期刊: Critical care medicine
• 影响因子: 8.8
• 作者: Arnold,JH;Stenz,RI;Grenier,B;Thompson,JE;Arnold,LW
• 通讯作者: Arnold,LW

Noninvasive determination of cardiac output using single breath CO2 analysis.

使用单次呼吸 CO2 分析无创测定心输出量。

• DOI: 10.1097/00003246-199610000-00017
• 发表时间: 1996
• 期刊: Critical care medicine
• 影响因子: 8.8
• 作者: Arnold,JH;Stenz,RI;Thompson,JE;Arnold,LW
• 通讯作者: Arnold,LW