Analysis of gene expression following maxillofacial inflammation in rat using complementary DNA microarray

使用互补 DNA 微阵列分析大鼠颌面部炎症后的基因表达

• 批准号: 15390628
• 负责人: KONDO Eiji
• 金额: $ 8.06万
• 依托单位: Matsutnoto Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15390628/
• 关键词: inflammation; trigeminal ganglion; cDNA array; inferior alveolar nerve; CaMKII; TRPV4; KCNK3; Prolactin; 三叉神経説; 三叉神経脊髄路核; プロラクチン; Trigeminal ganglion; complimentary DNA array; Complete Freund's Adjuvant; Capsaicin; Chloride Channel; Potass

To identify inflammation specific gene expression at trigeminal innervating region, we used complementary DNA microarray 20500 genes. Rats were injected Complete Freund's Adjuvant (CFA) into right superior lip. Trigeminal ganglion was extracted after 3days, and purified total RNA. The total RNA was labeled aminoalkyl-d UTP and conjugated Cy-dye fluorescence. The labeled RNA hybridized 20500 probes on Agilent Rat Oligo Microarray at 60 ℃ for 17h. We used scanning and analyzing specific gene expression by Agilent Scanner. We detected about 500 genes increased expression level under inflammation. In addition, two genes (CaMKII, KCNK3) were inspected by in situ hybridization.Furthermore we used this new powerful technology, c DNA array, to identify genes that were altered after inferior alveolar nerve (IAN) injury. The response to IAN injury is known to express hyperalgesia at maxillary nerve innervating region. We detected change in expression of about 200 genes after IAN injury. These genes may play a role in the response to inflammation and IAN nerve injury.

为了确定炎症特异性基因在三叉神经支配区域的表达，我们使用了互补基因芯片20500个基因。大鼠右上唇注射完全弗氏佐剂(CFA)。3d后取出三叉神经节，纯化总RNA。总RNA被标记为氨基-d-UTP和共轭细胞染料荧光。标记的核酸与20500个探针在60℃的Agilent大鼠寡核苷酸芯片上杂交17h。用Agilent扫描仪扫描和分析特定基因的表达。我们检测到大约500个基因在炎症条件下表达水平升高。此外，我们还通过原位杂交检测了两个基因(CaMKII、KCNK3)，并利用这一功能强大的新技术--c DNA阵列--来鉴定下牙槽神经损伤后发生改变的基因。已知对Ian损伤的反应表现为上颌神经支配区域的痛觉过敏。我们检测到约200个基因在Ian损伤后表达的变化。这些基因可能在对炎症和伊恩神经损伤的反应中发挥作用。

项目成果

Region-specific and epileptogenic-dependent expression of six subtypes of α2,3-sialyltransferase in the adult mouse brain

• DOI: 10.1046/j.1471-4159.2003.01257.x
• 发表时间: 2003-01-01
• 期刊: JOURNAL OF NEUROCHEMISTRY
• 影响因子: 4.7
• 作者: Matsuhashi, H;Horii, Y;Kato, K
• 通讯作者: Kato, K

Molecular Basis of Pain

疼痛的分子基础

• 发表时间: 2004
• 期刊: Matsumotoshigaku 30
• 作者: Abe;T;Kondo E.
• 通讯作者: Kondo E.

Evidence of parasympathetic postganglionic neurons in the rat hypoglossal nerve trunk

大鼠舌下神经干中副交感节后神经元的证据

• 发表时间: 2003
• 期刊: Experimental Brain Research 153・3
• 作者: Yasuda K;et al.
• 通讯作者: et al.

K.Yasuda, et al.: "Evidence of parasympathetic postganglionic neurons in the rat hypoglossal nerve trunk"Experimental Brain Research. 153・3. 302-309 (2003)

K. Yasuda等人：“大鼠舌下神经干中副交感神经节后神经元的证据”实验脑研究153・3（2003）。

最新の痛み分子基盤 -Capsaicin受容体を中心に-

疼痛的最新分子基础 - 聚焦辣椒素受体 -

• 发表时间: 2004
• 期刊: 松本歯学 30
• 作者: Toshio Sugahara;Takaaki Ueno et al.;金銅英二
• 通讯作者: 金銅英二