Analysis of gene expression following maxillofacial inflammation in rat using complementary DNA microarray

为了确定三叉神经支配区炎症特异性基因的表达，我们使用了20500个基因的互补DNA芯片。大鼠右上唇注射完全弗氏佐剂（CFA）。3天后提取三叉神经节，纯化总RNA。将总RNA标记为氨基烷基-d UTP，并结合cy -染料荧光。标记的RNA在Agilent Rat Oligo Microarray上60℃下杂交17h。我们使用安捷伦扫描仪扫描和分析特定的基因表达。我们检测到约500个基因在炎症下表达水平增加。此外，CaMKII、KCNK3两个基因通过原位杂交检测。此外，我们使用这种新的强大的技术，c - DNA阵列，鉴定下肺泡神经（IAN）损伤后改变的基因。对IAN损伤的反应表现为上颌神经支配区痛觉过敏。我们检测到大约200个基因在IAN损伤后的表达变化。这些基因可能在炎症反应和IAN神经损伤中发挥作用。

To identify inflammation specific gene expression at trigeminal innervating region, we used complementary DNA microarray 20500 genes. Rats were injected Complete Freund's Adjuvant (CFA) into right superior lip. Trigeminal ganglion was extracted after 3days, and purified total RNA. The total RNA was labeled aminoalkyl-d UTP and conjugated Cy-dye fluorescence. The labeled RNA hybridized 20500 probes on Agilent Rat Oligo Microarray at 60 ℃ for 17h. We used scanning and analyzing specific gene expression by Agilent Scanner. We detected about 500 genes increased expression level under inflammation. In addition, two genes (CaMKII, KCNK3) were inspected by in situ hybridization.Furthermore we used this new powerful technology, c DNA array, to identify genes that were altered after inferior alveolar nerve (IAN) injury. The response to IAN injury is known to express hyperalgesia at maxillary nerve innervating region. We detected change in expression of about 200 genes after IAN injury. These genes may play a role in the response to inflammation and IAN nerve injury.