Engineering A Novel Bacterial Host for Biobutanol Production

我们的目标是开发一种新的细菌宿主，用于从可再生原料中生产1-丁醇。该策略的重点是改造一种梭菌（巴氏梭菌）。这种微生物具有许多令人满意的特征，使其成为有吸引力的发酵宿主（生长速度快，健壮性和良好的丁醇耐受性），但底物范围有限，丁醇产量相对较差，并且难以遗传操纵。在这个项目中，我们将利用先进的分子生物学工具为梭状芽孢杆菌介绍淀粉代谢和丁醇生产的合成代谢途径。相互竞争的副产品将被淘汰。交付物将是一种新的工程菌株巴氏杆菌，它可以将淀粉发酵成丁醇，产量很高。

We aim to develp a novel bacterial host for the production of 1-butanol from renewable feedstocks. The strategy focuses on the modification of a clostridia species (Clostridium pasteurianum). This microbe has many desirable features that make it an attractive fermentation host (fast growth rates, robustness and good butanol tolerance) but suffers from a limited substrate range, relatively poor butanol yields and is notriously difficult to genetically manipulate. In this project, we will deploy advanced molecular biology tools for Clostridia to introduce synthetic metabolic pathways for starch metabolism and also for butanol production. Competing byproducts will be knocked out. The deliverable will be a novel engineered strain C. pasteurianum that ferments starch to butanol in high yield.