NHLBI MINORITY SCHOOL DEVELOPMENT AWARD

NHLBI 少数族裔学校发展奖

• 批准号: 3088423
• 负责人: STEVEN A EDWARDS
• 金额: $ 7.88万
• 依托单位: MEHARRY MEDICAL COLLEGE
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-07-01 至 1994-03-15
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3088423
• 关键词: gene expression; minority institution research support; nucleic acid sequence; oligonucleotides; posttranslational modifications; regulatory gene; transcription factor

Egr-l, EGR2, and EGR3 are early response genes, meaning that they are rapidly and transiently stimulated by mitogens, growth factors, and a variety of other stimuli. They are also zinc finger containing transcription factors which bind specifically to the sequence GCGGGGCG, a specificity which is shared by two related factors, NGF1-C, and the product of the Wilm's tumor locus, WT33. There is evidence to suggest that egr-l is involved in establishing and maintaining differentiated function in cardiac muscle and I neural cell types. The goals of this research project are: 1) To determine whether the expression of egr-like transcription factors are critical to growth control and differentiation and to identify potential target genes. 2) To determine the function of particular protein domains of egr-l. 3) To characterize and determine the function of post-translational modifications of egr-l. Results obtained in these experiments should help elucidate how differentiation pathways are determined and how differentiated function of certain tissues is maintained. This research should also lead to a greater understanding of the egr family of transcriptional regulators. If awarded, the proposed grant will support and maintain a research effort at Meharry Medical College focused on the field of transcriptional regulation. This will broaden the available opportunities for training minority students in the Biochemistry Department. The formal relationship proposed with Dr. Daryl Granner of Vanderbilt University will afford reciprocal training opportunities and technology transfer between the two schools.

Egr-1、EGR 2和EGR 3是早期应答基因，这意味着它们是 快速和短暂刺激的有丝分裂原，生长因子，和 各种各样的刺激。 它们也含有锌指 特异性结合序列GCGGGCG的转录因子， 特异性，由两个相关因子，NGF 1-C和 Wilm肿瘤基因座的产物，WT 33。 有证据表明 EGR-L参与建立和维持分化的 在心肌和神经细胞类型中的功能。 本研究的目标是：1）确定是否 EGFR样转录因子的表达对生长至关重要 控制和分化以及鉴定潜在的靶基因。2)到 确定EGFR-1的特定蛋白质结构域的功能。3)到 表征和确定翻译后的功能 EGR-L的修饰。 在这些实验中获得的结果应该 有助于阐明分化途径是如何确定的， 某些组织的分化功能得以维持。 本研究 也应该导致更好地了解家庭的 转录调节因子。 如果被授予，拟议的赠款将支持和维持一项研究， Meharry医学院的努力集中在转录领域， 调控 这将扩大现有的培训机会 生物化学系的少数民族学生。 正式 与范德比尔特大学的达里尔·格兰纳博士提出的关系 将提供互惠的培训机会和技术转让 两所学校之间。